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Chemiluminescent ELISAs

Article

Luminescent immunoassays combine great sensitivity with broad dynamic range. One of the more effective luminescent indicators is Luminol, which, with proper enhancers, gives a bright and stable light emission. Figure 1. Enhanced Luminol Reaction Luminol (3-aminophthal hydrazide) was first synthesized as a chemiluminescent substrate in 1928. Unfortunately, the...

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DuoSet® ELISA Development Systems - ELISA Kits

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Download 384-Well Technical Note View Complete Listing of DuoSet Development Systems DuoSet ELISA Development Systems contain the basic components required to develop a sandwich immunoassay for accurately measuring analytes in biological fluids. When complete kits are not an option, DuoSet ELISA Development Kits are an economical alternative to buying separate antibodies and protein...

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Custom ELISA Assay Services

Services

Harness the unique experience and expertise of the R&D Systems Quantikine® ELISA team Testimonial    Mellitus was looking for a development partner to assist the Company in converting its research assay to a commercially viable in vitro diagnostic. We had firsthand knowledge of the exquisite technical expertise residing within R&D Systems. With its...

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HT 8-oxo-dG ELISA Kits

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The HT 8-oxo-dG ELISA is a plate-based immunoassay designed for detecting and quantifying 8-hydroxy-2’-deoxyguanosine (8-oxo-dG). 8-oxo-dG is a frequently used biomarker of oxidative DNA damage and oxidative stress. Reactive oxygen and nitrogen species generated by normal metabolic processes and a variety of environmental factors cause the hydroxylation of guanosine at the N7-C8 bond....

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Parameter Colorimetric Competitive ELISAs

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Complete Listing of Parameter Assay Kits The same quality and performance researchers have come to expect using our Quantikine® line of ELISA Kits has now been applied to R&D Systems Parameter Kits. These kits are complete microplate-based assays designed to accurately measure the levels of small molecules including cAMP, cGMP, NO, eicosanoids, creatinine, and more. Features Fully...

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Supplemental ELISA Development Products

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Complete Listing of Supplemental ELISA Development Products Clear Microplates (Catalog # DY990) A 25 pack of clear high-binding, flat-bottom polystyrene microplates (360 µL/well). Each 96-well microplate consists of 12 removable strips of 8 wells and a frame. Clear Microplates (Catalog # DY990/384) A 25 pack of clear high-binding, flat-bottom polystyrene 384-well microplates (360...

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Troubleshooting Guide: ELISA

Protocols

Researchers can encounter a variety of problems when running an ELISA. Explore this page to understand the potential source of your problem and the steps we recommend for correcting the issue to help set you on a path for running a successful ELISA. For further assistance, please contact our technical service department. High background No signal Too much signal - whole plate turned...

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ELISA Tips and Tricks Poster

Mini Posters

To request your Elisa Tips and Tricks wall poster, please click the button below to go to the poster request page on www.bio-techne.com. Thank you! Request Poster

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PlGF ELISA for Preeclampsia Research

Article

Figure 1. Typical standard curve generated using R&D Systems Human PlGF Quantikine ELISA (Catalog # DPG00). These data are for demonstration purposes only. A standard curve should be generated for each set of samples assayed.   Figure 2. Human serum, plasma, and urine samples were assayed for PlGF using R&D Systems Human PlGF Quantikine ELISA (Catalog #...

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QuantiGlo® Chemiluminescent Sandwich ELISA Kits

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  Assay Principle View Full Assay Principle Complete Listing of QuantiGlo ELISA Kits QuantiGlo ELISA Kits use a chemiluminescent substrate for analyte detection and require a luminometer for output reading. These kits have a broad dynamic range in comparison to standard colorimetric...

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