"organoid proteins" has 26 results in Resources.

Optimizing Organoid Culture Conditions Identifying and optimizing the conditions for culturing organoids can be technically challenging. Organoid cultures typically contain multiple components and can be highly variable for different types of organoids. As a result, it can take time, and a lot of trial and error to find the appropriate conditions to support robust organoid...

This protocol outlines a method for culturing normal human intestinal organoids based on protocols described by C. Pleguezuelos-Manzano and Hans Clevers (1). It is intended to culture organoids from normal human intestinal tissues using Cultrex™ UltiMatrix RGF Basement Membrane Extract as a scaffold, along with recombinant proteins, small molecules, and media supplements available from...

This protocol outlines a method for culturing normal human liver organoids based on protocols described by Meritxell Huch (1). Starting with a suspension of isolated and dissociated human liver tissue, the reagents and steps necessary to generate and passage human liver organoids are fully detailed. This protocol provides a method for generating an in vitro liver model that can be used for...

This protocol outlines a method for culturing normal human lung organoids based on protocols described by Norman Sachs and Hans Clevers (1). The procedure that is described allows for the long-term expansion of airway organoids in vitro from adult human lung tissue. Starting with a suspension of isolated and dissociated human lung tissue, the reagents and steps necessary to generate and...

This protocol outlines a method for the harvesting of organoids from culture as well as the processing recommendations for passaging and biochemical analysis using the Cultrex® Organoid Harvesting Solution. Some specific tips and considerations you can find in this protocol include: Method for the non-enzymatic depolymerization of extracellular matrix proteins for passaging and analysis...

Bio-Techne provides an extensive range of reagents to cater to diverse cell culture needs spanning from fundamental research and drug discovery to clinical manufacturing. We offer basement membrane extracts, specialty media and media supplements, small molecules, and research-grade, Animal-free preclinical, and GMP-grade recombinant proteins. These products are designed to promote robust and...

Most stem cell research depends on the ability to culture different stem cell types and induce their differentiation in vitro. Download this guide to discover the key benefits of our proteins for stem cell culture and learn how these products can help reduce challenges with consistency and scalability in your stem cell research and therapeutic programs. Products detailed in this guide...

Basement Membrane Extract (also referred to as BME, extracellular matrix (ECM), or basement membrane hydrogel) is used globally for culture applications including organoid generation and pluripotent stem cell expansion. While BME is universally utilized as a scaffolding matrix for 2D and 3D cell culture, researchers spend tremendous amounts of effort testing and identifying specific lots of...

Organoids are emerging as powerful model systems for investigating human organ development, modeling diseases, screening potential drugs, and exploring personalized medicine. While many scientists are now interested in using organoid models for their research, identifying the ideal conditions for culturing organoids can be difficult. This is primarily due to complex, multi-component...

To resolve the challenges of purifying hydrophobic Wnt proteins in an active state and using multiple proteins in a single experiment, R&D Systems™ has engineered a Wnt agonist protein that can act as a Wnt and R-Spondin pathway activator to promote canonical Wnt signaling. As seen in this application note, Wnt/R-Spondin Agonist Proteins display better activity in a Wnt/β-Catenin assay...