"pi3k" has 77 results in Resources.

  Flow Cytometry Cell Viability Overview Flow cytometry is a quick and reliable method to quantify viable cells. Determining cell viability is an important step when evaluating a cells response to drug treatments or other environmental factors. It is also often necessary to distinguish dead cells in a cell suspension in order to exclude them from analysis. Dead cells can...

Click for larger image. The importance of both Ca2+ and Pi in the body is reflected in the exquisite hormonal regulation associated with each element. Calcium (Ca2+) represents approximately 1-2% of total body weight, while phosphorus (Pi) constitutes approximately half that amount. Although most Ca2+ (99%) and Pi (85%) exist as a complex within bone, each has a number of critical non-structural...

TGF-ß, the prototypical member of the TGF-ß superfamily, acts as a potent growth inhibitor or stimulator of apoptosis in many cell types. The downstream signaling initiated by TGF-ß binding to its serine/threonine kinase receptor is carried out by members of the Smad family. In general, activation of Smad2 or Smad3 results in the formation of a heteromeric complex with Smad4 that translocates...

Prostate cancer (PCa) is the most common noncutaneous malignancy in men in the US. A significant fraction of advanced PCa treated with androgen deprivation therapy experience relentless progression to lethal metastatic castration-resistant prostate cancer (mCRPC). The PCa tumor microenvironment is comprised of a complex mixture of epithelial and stroma cell types engaged in multifaceted...

PI 3-Kinase can be activated by numerous stimuli, including mitogen-stimulated receptor tyrosine kinases (RTKs). The PI 3-Kinase p85 regulatory subunit interacts with RTKs either directly via its Src-homology 2 (SH2) domains or indirectly via an adaptor protein, such as GAB. Activated PI 3-Kinase then phosphorylates phosphatidylinositol (4,5)-bisphosphate (PIP2), resulting in the formation of...

First Published in R&D Systems' 2007 Catalog Contents Intestinal Absorption Calcium Phosphorus Serum Calcium & Phosphorus Molecules Regulating Calcium & Phosphorus Metabolism Renal Resporption of Calcium and Phosphorus Bone Formation and Resporption Elementary physiology and biochemistry texts generally do not have sections dedicated to inorganic elements or metals. This is not...

Macroautophagy, hereafter referred to as autophagy, is a catabolic process by which cells degrade long-lived proteins, organelles, and certain types of bacteria via lysosomes. Briefly, the autophagy pathway entails the development of a phagophore that envelopes cytoplasmic components and forms a double-membrane autophagosome that subsequently fuses with a lysosome for the digestion of its...

Greta Wegner, Erin Eleria, Jackie Ernst, & Amy James THE PROBLEM: Time-consuming Western blots Assessing phosphorylation changes in multiple interrelated pathways Performing pharmacological screens in a timely manner Identifying off-target pharmacological effects THE SOLUTION: Proteome Profiler™ Phospho-Antibody Arrays Easier to perform than a Western blot Uncover new...

Jennifer Ferrer, Graduate student Chicago IL, USA Salma Jalal, Graduate student Singapore Renfang Mao, Associated Professor Nantong, CHINA Colleen McSweeney, Graduate Student Penn State University, USA Somnath Mukherjee, Postdoctoral Fellow New Delhi, INDIA   Raymond Wong, Graduate Student Toronto, CANADA Hannah Schwender, Graduate...

Protein kinases play critical roles in signal transduction, and their physiological activities in cellular processes are important in defining signaling pathways. Likewise, unregulated activities of protein kinases are of particular interest in understanding pathological pro­cesses such as cancer and chronic inflam­matory diseases. Protein kinases often have multiple physiological substrates...