"rtk" has 24 results in Resources.

Reagent Preparation Bring all reagents to room temperature before use. Human Phospho-RTK Array - Four nitrocellulose membranes each containing 42 different anti-RTK antibodies and 6 controls printed in duplicate. Handle arrays only with gloved hands and flat-tipped tweezers. After opening, reseal unused membranes in the foil pouch with desiccant and store at 2 - 8° C for up to 3...

R&D Systems™ Proteome Profiler Human Phospho-RTK Array Kit (Catalog # ARY001B) detects multiple tyrosine phosphorylated receptors in untreated and ligand-treated cell lysates. The amount of each lysate used is indicated by each cell line tested. Array signals from scanned X-ray film images were analyzed using image analysis software. A. Treatment of human HepG2 liver cell line with insulin...

Jane Schmidt, Ph.D., Judy Haning, Shaya Anderson, & Richard K. Fuerstenberg INTRODUCTION Receptor Tyrosine Kinases (RTKs) are widely expressed transmembrane receptors for growth factors and other extracellular signaling molecules. Binding of a specific ligand leads to phospho-rylation at specific sites in the cytoplasmic tail that triggers further signal transduction processes. RTKs play...

Recent Studies Investigate Pathways Mediating Drug Resistance in Cancer Cells Since activation of a number of different signaling pathways may promote the cancer phenotype, screening tools that simultaneously assess the phosphorylation state of multiple proteins can be extremely useful. R&D Systems Proteome Profiler Human Phospho-RTK Antibody Array (Catalog # ARY001B) and Phospho-Kinase Array...

Can other lysis buffers be used? Can a non-film based image acquisition system, such as a cooled CCD camera, be used? Can the array membranes be stripped and reused? What exposure times should be used for chemiluminescent detection with X-ray film? Can the sensitivity be increased by altering the conjugate concentration? What is the sensitivity of the Phospho-RTK Array? Can tissue...

Can other lysis buffers be used? The kit has been validated for use with NP-40 Lysis Buffer.We cannot guarantee that the assay performance will be acceptable if other lysis buffers are used. Can a non-film based image acquisition system, such as a cooled CCD camera, be used? Yes. You will have to follow the instrument manufacturer’s protocol and optimize the exposure length. Can the array...

Receptor Tyrosine Kinases (RTKs) are widely expressed transmembrane proteins that act as receptors for growth factors, neurotrophic factors, and other extracellular signaling molecules. Upon ligand binding, they undergo tyrosine phosphorylation at specific residues in the cytoplasmic tail. This leads to the binding of protein substrates and/or the establishment of docking sites for adaptor...

Receptor Tyrosine Kinases (RTKs) are widely expressed transmembrane proteins that act as receptors for growth factors, neurotrophic factors, and other extracellular signaling molecules. Upon ligand binding, they undergo tyrosine phosphorylation at specific residues in the cytoplasmic tail. This leads to the binding of protein substrates and/or the establishment docking sites for adaptor proteins...

Mouse Phospho-RTK Array Kit Ordering Information Introduction R&D Systems' Mouse Phospho-Receptor Tyrosine Kinase (RTK) Array Kit (Catalog # ARY014) is a screening tool designed to simultaneously detect the relative phosphorylation of 39 different RTKs. The Kit eliminates the need for numerous immunoprecipitation (IP) and/or Western blot experiments. RTKs are captured by antibodies spotted...

Human Phospho-RTK Array Kit Ordering Information Introduction R&D Systems™ Human Phospho-Receptor Tyrosine Kinase (RTK) Array Kit (Catalog # ARY001B) is a screening tool designed to simultaneously detect the relative phosphorylation of 49 different RTKs. This kit eliminates the need for numerous immunoprecipitation (IP) and/or Western blot experiments. RTKs are captured by antibodies...