The Cloudz T Cell Activation Kit - CD3/CD28 contains non-magnetic, dissolvable microparticles that are manufactured under GMP controls and conjugated with the common T cell-activating antibodies, CD3 and CD28. This kit improves T cell expansion and simplifies cell recovery for ex vivo manufacturing, while also providing safety and efficacy advantages over current bead-based T cell activation methods.

Features

• CD3/CD28 antibody-conjugated microparticles
• Release buffer dissolves particles in < 10 seconds
• Manufactured under GMP controls

Benefits

• Ideal for CAR T or T cell therapeutic manufacturing
• Robust T cell activation
• Simplified recovery of cells
• Improved product purity over magnetic activation techniques

Cloudz CD3/CD28 Protocol

Advantages of Cloudz CD3/CD28 for Cell Therapy

Robust CD3⁺ T Cell Expansion

Cloudz CD3/CD28 induces 8-9 population doublings during a 9-day ex vivo culture of human CD3⁺ T cells or PBMCs.

Favorable Performance Over Magnetic Bead and Soluble Antibody

With comparable expansion rates to existing bead and soluble antibody activating protocols, Cloudz CD3/CD28 offers additional manufacturing advantages, including single administration use and easier cell collection.

Improved CD8⁺ T Cell Expansion

Cloudz CD3/CD28 improves CD8⁺ effector T cell expansion without compromising expansion rates of CD4⁺ T cells.

Externally Validated

Independent analysis of Cloudz CD3/CD28 shows improved expansion over alternative expansion technologies. *Results disclosed with permission of external collaborator.

Improved T Cell Transduction

Cloudz CD3/CD28 shows improved CAR transduction in CD3⁺, CD4⁺, and CD8⁺ T cells compared to bead-based technologies. *Results disclosed with permission of external collaborator.