Coupling Rates for 10 Minute Kinase Reactions

The following table lists the coupling rates for 10 minute kinase reactions coupled with Recombinant Mouse CD39L2.  The coupling rates are for 50 μL reactions carried out at room temperature in a buffer consisting of 25 mM HEPES, 10 mM CaCl2, 10 mM MgCl2, 150 mM NaCl, pH 7.0. The coupling rates were calculated using a specific rate constant of 97.78 nmol/min/μg/mM.

The red shaded region represents the optimal range for coupling rates in order to achieve the maximum signal-to-noise ratio for the phosphatase-coupled kinase reaction.

For more detailed technical information, please also see: Wu, Z.L. (2011) PLoS ONE 6(8):e23172.

Coupling Rates for 10 Minute Kinase Reactions

ATP
(μM)		 CD39L2 (μg)
0.025 0.050 0.075 0.100 0.125 0.150 0.175 0.200
50 0.183 0.323 0.431 0.515 0.581 0.634 0.677 0.712
100 0.174 0.309 0.415 0.498 0.564 0.618 0.662 0.697
150 0.168 0.299 0.403 0.486 0.552 0.606 0.650 0.686
200 0.163 0.291 0.394 0.475 0.542 0.596 0.640 0.677
250 0.159 0.285 0.386 0.467 0.533 0.587 0.632 0.669
300 0.155 0.279 0.379 0.460 0.525 0.580 0.625 0.662
400 0.149 0.269 0.367 0.447 0.512 0.567 0.612 0.650
500 0.144 0.262 0.357 0.436 0.501 0.556 0.601 0.640
600 0.140 0.255 0.349 0.427 0.492 0.546 0.592 0.631
700 0.137 0.249 0.342 0.419 0.483 0.537 0.583 0.622
800 0.133 0.244 0.335 0.411 0.476 0.530 0.576 0.615
900 0.130 0.239 0.329 0.405 0.468 0.522 0.568 0.608
1000 0.128 0.234 0.324 0.399 0.462 0.516 0.562 0.601
1100 0.125 0.230 0.319 0.393 0.456 0.510 0.556 0.595
1200 0.123 0.227 0.314 0.388 0.450 0.504 0.550 0.590
1300 0.121 0.223 0.310 0.383 0.445 0.499 0.544 0.584
1400 0.119 0.220 0.305 0.378 0.440 0.493 0.539 0.579
1500 0.118 0.217 0.302 0.374 0.436 0.489 0.535 0.574
1600 0.116 0.214 0.298 0.370 0.431 0.484 0.530 0.570
1700 0.114 0.212 0.295 0.366 0.427 0.480 0.525 0.565
1800 0.113 0.209 0.291 0.362 0.423 0.476 0.521 0.561
1900 0.111 0.207 0.288 0.358 0.419 0.472 0.517 0.557
2000 0.110 0.204 0.285 0.355 0.415 0.468 0.513 0.553
2100 0.109 0.202 0.282 0.352 0.412 0.464 0.510 0.549
2200 0.108 0.200 0.280 0.349 0.408 0.460 0.506 0.546
2300 0.106 0.198 0.277 0.346 0.405 0.457 0.502 0.542
2400 0.105 0.196 0.275 0.343 0.402 0.454 0.499 0.539
2500 0.104 0.194 0.272 0.340 0.399 0.451 0.496 0.536
2600 0.103 0.193 0.270 0.337 0.396 0.448 0.493 0.532
2700 0.102 0.191 0.268 0.335 0.393 0.445 0.490 0.529
2800 0.101 0.189 0.266 0.332 0.391 0.442 0.487 0.526
2900 0.100 0.188 0.264 0.330 0.388 0.439 0.484 0.523
3000 0.100 0.186 0.261 0.327 0.385 0.436 0.481 0.521