Coupling Rates for 30 Minute Kinase Reactions

The following table lists the coupling rates for 30 minute kinase reactions coupled with Recombinant Mouse CD39L2. The coupling rates are for 50 μL reactions carried out at room temperature in a buffer consisting of 25 mM HEPES, 10 mM CaCl2, 10 mM MgCl2, 150 mM NaCl, pH 7.0. The coupling rates were calculated using a specific rate constant of 97.78 nmol/min/μg/mM.

The red shaded region represents the optimal range for coupling rates in order to achieve the maximum signal-to-noise ratio for the phosphatase-coupled kinase reaction.

For more detailed technical information, please also see: Wu, Z.L. (2011) PLoS ONE 6(8):e23172.

Coupling Rates for 30 Minute Kinase Reactions

ATP
(μM)		 CD39L2 (μg)
0.025 0.038 0.050 0.063 0.075 0.088 0.100 0.125 0.150 0.175 0.200
50 0.431 0.550 0.634 0.696 0.741 0.776 0.802 0.841 0.868 0.886 0.901
100 0.415 0.533 0.618 0.680 0.727 0.763 0.791 0.832 0.860 0.880 0.895
150 0.403 0.521 0.606 0.669 0.717 0.754 0.783 0.825 0.854 0.875 0.890
200 0.394 0.510 0.596 0.660 0.708 0.746 0.776 0.819 0.849 0.870 0.886
250 0.386 0.502 0.587 0.652 0.701 0.739 0.769 0.814 0.844 0.866 0.883
300 0.379 0.494 0.580 0.644 0.694 0.733 0.764 0.809 0.840 0.863 0.880
400 0.367 0.481 0.567 0.632 0.682 0.722 0.754 0.801 0.833 0.857 0.875
500 0.357 0.470 0.556 0.621 0.672 0.713 0.745 0.793 0.827 0.851 0.870
600 0.349 0.461 0.546 0.612 0.664 0.705 0.738 0.787 0.821 0.847 0.866
700 0.342 0.452 0.537 0.604 0.656 0.697 0.731 0.781 0.816 0.842 0.862
800 0.335 0.445 0.530 0.596 0.648 0.690 0.724 0.776 0.812 0.838 0.858
900 0.329 0.438 0.522 0.589 0.642 0.684 0.718 0.770 0.807 0.834 0.855
1000 0.324 0.432 0.516 0.582 0.635 0.678 0.713 0.766 0.803 0.830 0.851
1100 0.319 0.426 0.510 0.576 0.629 0.672 0.708 0.761 0.799 0.827 0.848
1200 0.314 0.420 0.504 0.570 0.624 0.667 0.703 0.757 0.795 0.824 0.845
1300 0.310 0.415 0.499 0.565 0.619 0.662 0.698 0.753 0.792 0.821 0.843
1400 0.305 0.410 0.493 0.560 0.614 0.657 0.693 0.749 0.788 0.818 0.840
1500 0.302 0.406 0.489 0.555 0.609 0.653 0.689 0.745 0.785 0.815 0.837
1600 0.298 0.402 0.484 0.550 0.604 0.649 0.685 0.741 0.782 0.812 0.835
1700 0.295 0.397 0.480 0.546 0.600 0.644 0.681 0.738 0.779 0.809 0.832
1800 0.291 0.394 0.476 0.542 0.596 0.640 0.677 0.734 0.776 0.806 0.830
1900 0.288 0.390 0.472 0.538 0.592 0.636 0.674 0.731 0.773 0.804 0.828
2000 0.285 0.386 0.468 0.534 0.588 0.633 0.670 0.728 0.770 0.801 0.826
2100 0.282 0.383 0.464 0.530 0.584 0.629 0.667 0.725 0.767 0.799 0.823
2200 0.280 0.380 0.460 0.526 0.581 0.626 0.663 0.722 0.764 0.797 0.821
2300 0.277 0.376 0.457 0.523 0.577 0.622 0.660 0.719 0.762 0.794 0.819
2400 0.275 0.373 0.454 0.520 0.574 0.619 0.657 0.716 0.759 0.792 0.817
2500 0.272 0.371 0.451 0.516 0.571 0.616 0.654 0.713 0.757 0.790 0.815
2600 0.270 0.368 0.448 0.513 0.567 0.613 0.651 0.711 0.754 0.788 0.813
2700 0.268 0.365 0.445 0.510 0.564 0.610 0.648 0.708 0.752 0.785 0.811
2800 0.266 0.362 0.442 0.507 0.561 0.607 0.645 0.705 0.750 0.783 0.810
2900 0.264 0.360 0.439 0.504 0.558 0.604 0.642 0.703 0.748 0.781 0.808
3000 0.261 0.357 0.436 0.501 0.556 0.601 0.640 0.700 0.745 0.779 0.806