Figure 1. The Human Cytokine Array detects multiple analytes in cell culture supernates. A. Human Peripheral Blood Mononuclear Cells (PBMCs) treated with 10 µg/mL PHA for 24 hours. B. TF-1 human erythroleukemic cell line treated with 50 ng/mL PMA for 18 hours. C. THP-1 human acute monocytic leukemia cell line treated with 1 µg/mL LPS for 18 hours. In each array shown, 500 µL of cell supernate was used. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System. |
Figure 2. The Human Cytokine Array detects multiple analytes in supernates and serum. A. and B. Human Peripheral Blood Mononuclear Cells (PBMCs) were untreated or treated with 50 ng/mL PMA for 24 hours. In each array shown, 500 µL of cell culture supernate was used. C. Human serum (165 µL). Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System. |
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