Recombinant Human TGF-beta RII Protein Summary
Product Specifications
Ile24-Asp159
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
241-R2
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
241-R2/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Scientific Data
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Recombinant human TGF-beta RII (241-R2) inhibits recombinant human TGF-beta 1 activity in the HT-2 mouse T cell line. The ED50 for this effect is 1-3 µg/mL in the presence of 0.25 ng/mL of recombinant human TGF-beta 1.
Reconstitution Calculator
Background: TGF-beta RII
Most cell types express three sizes of receptors for TGF-beta. These are designated type I (53 kDa), type II (70-85 kDa), and type III (250-350 kDa). The type III receptor, a proteoglycan that exists in membrane-bound and soluble forms, binds TGF-beta 1, TGF-beta 2, and TGF-beta 3 but does not appear to be involved in signal transduction. The type II receptor is a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The type I receptor is also a membrane-bound serine/threonine kinase that apparently requires the presence of the type II receptor to bind TGF-beta. Current evidence suggests that signal transduction requires the cytoplasmic domains of both the type I and type II receptors.
The recombinant soluble TGF-beta type II receptor is capable of binding TGF-beta 1, TGF-beta 3, and TGF-beta 5 with sufficient affinity to act as an inhibitor of these isoforms at high concentrations. The soluble receptor also binds TGF-beta 2, but with an affinity at least two orders of magnitude lower. Binding of TGF-beta 1, TGF-beta 3, and TGF-beta 5 to the soluble TGF-beta type II receptor can also be demonstrated by using the soluble receptor as a capture agent on ELISA plates and this observation has been used as the basis for the development of immunoassays for these isoforms of TGF-beta.
Citations for Recombinant Human TGF-beta RII Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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IMM-H007 attenuates isoprenaline-induced cardiac fibrosis through targeting TGFbeta1 signaling pathway
Authors: SX Wang, YN Feng, S Feng, JM Wu, M Zhang, WL Xu, YY Zhang, HB Zhu, H Xiao, ED Dong
Acta pharmacologica Sinica, 2022-03-30;0(0):.
Species: Mouse
Sample Types: Complex Sample Type
Applications: Bioassay -
Serine protease HTRA1 antagonizes transforming growth factor-beta signaling by cleaving its receptors and loss of HTRA1 in vivo enhances bone formation.
Authors: Graham J, Chamberland A, Lin Q, Li X, Dai D, Zeng W, Ryan M, Rivera-Bermudez M, Flannery C, Yang Z
PLoS ONE, 2013-09-11;8(9):e74094.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
Antibody-mediated blockade of integrin alpha v beta 6 inhibits tumor progression in vivo by a transforming growth factor-beta-regulated mechanism.
Authors: Van Aarsen LA, Leone DR, Ho S, Dolinski BM, McCoon PE, LePage DJ, Kelly R, Heaney G, Rayhorn P, Reid C, Simon KJ, Horan GS, Tao N, Gardner HA, Skelly MM, Gown AM, Thomas GJ, Weinreb PH, Fawell SE, Violette SM
Cancer Res., 2008-01-15;68(2):561-70.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Transforming growth factor beta1 regulates follistatin mRNA expression during in vitro bovine granulosa cell differentiation.
Authors: Fazzini M, Vallejo G, Colman-Lerner A, Trigo R, Campo S, Baranao JL, Saragueta PE
J. Cell. Physiol., 2006-04-01;207(1):40-8.
Species: Bovine
Sample Types: Whole Cells
Applications: Bioassay -
Transforming growth factor-beta1 regulation of growth zone chondrocytes is mediated by multiple interacting pathways.
Authors: Rosado E, 2019, Schwartz Z, Sylvia VL, Dean DD, Boyan BD
91, 2002-06-12;1590(1):1-15.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay
FAQs
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Can Recombinant Human TGF-β RII (Catalog # 241-R2) be used as a capture reagent in an ELISA-like assay?
Reviews for Recombinant Human TGF-beta RII Protein
Average Rating: 4.7 (Based on 3 Reviews)
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