Rat IgG Horseradish Peroxidase-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of Rat IgG Primary Antibody by Western Blot. Western blot shows detection of Rat Anti-Human/Mouse Wnt-5a Monoclonal Antibody (MAB645) using a 1:1000 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Rat IgG Primary Antibody by Simple WesternTM. Simple Western lane view shows detection of Rat Anti-Mouse S100A9 Monoclonal Antibody ( MAB2065) using a 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Rat IgG Primary Antibody by Immunohistochemistry. Detection of Vimentin in human kidney using 5 ug/mL of Rat monoclonal anti-human Vimentin primary antibody (MAB2105) followed by 30 min incubation with Goat anti Rat HRP conjugated secondary antibodies (Catalog # HAF005) at 1:20 dilution + DAB chromogen (brown). The tissue was counterstained with Hematoxylin (blue). Control was done by omitting primary antibody.
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Preparation and Storage
- 6 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Product Datasheets
Citations for Rat IgG Horseradish Peroxidase-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Ezrin Binds to DEAD-Box RNA Helicase DDX3 and Regulates Its Function and Protein Level
Authors: Haydar Çelik, Kamal P. Sajwan, Saravana P. Selvanathan, Benjamin J. Marsh, Amrita V. Pai, Yasemin Saygideger Kont et al.
Molecular and Cellular Biology
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Identification of the NRF2 transcriptional network as a therapeutic target for trigeminal neuropathic pain
Authors: C Vasavda, R Xu, J Liew, R Kothari, RS Dhindsa, ER Semenza, BD Paul, DP Green, MF Sabbagh, JY Shin, W Yang, AM Snowman, LK Albacarys, A Moghekar, CA Pardo-Vill, M Luciano, J Huang, C Bettegowda, SG Kwatra, X Dong, M Lim, SH Snyder
Science Advances, 2022-08-03;8(31):eabo5633.
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Sodium Iodate-Induced Degeneration Results in Local Complement Changes and Inflammatory Processes in Murine Retina
Authors: Anne Enzbrenner, Rahel Zulliger, Josef Biber, Ana Maria Quintela Pousa, Nicole Schäfer, Corinne Stucki et al.
International Journal of Molecular Sciences
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Interleukin 33 regulates gene expression in intestinal epithelial cells independently of its nuclear localization
Authors: Z He, L Chen, GC Furtado, SA Lira
Cytokine, 2018-08-23;111(0):146-153.
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An agonistic anti-signal regulatory protein alpha antibody for chronic inflammatory diseases
Authors: Markus M. Xie, Bingbing Dai, Jason A. Hackney, Tianhe Sun, Juan Zhang, Janet K. Jackman et al.
Cell Reports Medicine
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Topical nanoparticles interfering with the DNA-LL37 complex to alleviate psoriatic inflammation in mice and monkeys
Authors: Liang H, Yan Y, Wu J et al.
Science Advances
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Cyclooxygenase inhibition attenuates brain angiogenesis and independently decreases mouse survival under hypoxia
Authors: Drew R. Seeger, Svetlana A. Golovko, Bryon D. Grove, Mikhail Y. Golovko
Journal of Neurochemistry
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Transcriptomic Analysis of Vulvovaginal Candidiasis Identifies a Role for the NLRP3 Inflammasome
Authors: Vincent M. Bruno, Amol C. Shetty, Junko Yano, Paul L. Fidel, Mairi C. Noverr, Brian M. Peters
mBio
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Allelic Interaction between CRELD1 and VEGFA in the Pathogenesis of Cardiac Atrioventricular Septal Defects
Authors: Jennifer K. Redig, Gameil T. Fouad, Darcie Babcock, Benjamin Reshey, Eleanor Feingold, Roger H. Reeves et al.
AIMS Genetics
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Tricellulin secures the epithelial barrier at tricellular junctions by interacting with actomyosin
Authors: Yuma Cho, Daichi Haraguchi, Kenta Shigetomi, Kenji Matsuzawa, Seiichi Uchida, Junichi Ikenouchi
Journal of Cell Biology
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mTORC2 suppresses cell death induced by hypo-osmotic stress by promoting sphingomyelin transport
Authors: Yumiko Ono, Kenji Matsuzawa, Junichi Ikenouchi
Journal of Cell Biology
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eIF4E-Binding Proteins 1 and 2 Limit Macrophage Anti-Inflammatory Responses through Translational Repression of IL-10 and Cyclooxygenase-2
Authors: M William, LP Leroux, V Chaparro, J Lorent, TE Graber, MN M'Boutchou, T Charpentie, A Fabié, CM Dozois, S Stäger, LC van Kempen, T Alain, O Larsson, M Jaramillo
J. Immunol., 2018-04-30;0(0):.
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Purinergic P2X7 Receptor Mediates the Elimination of Trichinella spiralis by Activating NF-kappa B/NLRP3/IL-1 beta Pathway in Macrophages
Authors: Fei Guan, Wangfang Jiang, Yang Bai, Xiao Hou, Chunjie Jiang, Chongyang Zhang et al.
Infection and Immunity
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Predicted molecular signaling guiding photoreceptor cell migration following transplantation into damaged retina
Authors: Uchenna John Unachukwu, Alice Warren, Ze Li, Shawn Mishra, Jing Zhou, Moira Sauane et al.
Scientific Reports
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Vascular architecture regulates mesenchymal stromal cell heterogeneity via P53-PDGF signaling in the mouse incisor
Authors: Guo, T;Pei, F;Zhang, M;Yamada, T;Feng, J;Jing, J;Ho, TV;Chai, Y;
Cell stem cell
Species: Transgenic Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
The cholesterol transporter NPC1 is essential for epigenetic regulation and maturation of oligodendrocyte lineage cells
Authors: Kunkel, TJ;Townsend, A;Sullivan, KA;Merlet, J;Schuchman, EH;Jacobson, DA;Lieberman, AP;
Nature communications
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Selective Inhibition of Soluble Tumor Necrosis Factor Alters the Neuroinflammatory Response following Moderate Spinal Cord Injury in Mice
Authors: Lund, MC;Ellman, DG;Nielsen, PV;Raffaele, S;Fumagalli, M;Guzman, R;Degn, M;Brambilla, R;Meyer, M;Clausen, BH;Lambertsen, KL;
Biology
Species: Mouse
Sample Types: Protein
Applications: Western Blot -
Toxoplasma gondii profilin induces NLRP3 activation and IL-1beta production/secretion in THP-1�cells
Authors: H Pazoki, H Mirjalali, M Niyyati, SJ Seyed Taba, N Mosaffa, S Shahrokh, H Asadzadeh, A Kupz, MR Zali
Microbial pathogenesis, 2023-04-18;180(0):106120.
Species: Human
Sample Types: Protein
Applications: Western Blot -
Dipeptidylpeptidase 4 promotes survival and stemness of acute myeloid leukemia stem cells
Authors: C Wang, R Nistala, M Cao, Y Pan, M Behrens, D Doll, RD Hammer, P Nistala, HM Chang, ETH Yeh, X Kang
Cell Reports, 2023-02-17;42(2):112105.
Species: Xenograft
Sample Types: Cell Lysates
Applications: Western Blot -
Toxoplasma gondii profilin induces NLRP3 activation and IL-1beta production/secretion in THP-1�cells
Authors: H Pazoki, H Mirjalali, M Niyyati, SJ Seyed Taba, N Mosaffa, S Shahrokh, H Asadzadeh, A Kupz, MR Zali
Microbial pathogenesis, 2023;180(0):106120.
Species: Human
Sample Types: Protein
Applications: Western Blot -
Novel p.G250A Mutation Associated with Chronic Pancreatitis Highlights Misfolding-Prone Region in Carboxypeptidase A1 (CPA1)
Authors: M Sándor, FG Thiel, M Schmid, A Demcsák, NC Morales Gr, BC Németh, S Vajda, A Hoerning, M Sahin-Tóth
International Journal of Molecular Sciences, 2022-12-07;23(24):.
Species: Human
Sample Types: Protein Lysate
Applications: Western Blot -
The expression of nerve growth factor in healthy and inflamed equine chondrocytes analysed by capillary western immunoassay
Authors: S Nyström, A Kendall, S Adepu, A Lindahl, E Skiöldebra
Research in Veterinary Science, 2022-08-22;151(0):156-163.
Species: Horse
Sample Types: Cell Lysates
Applications: Western Blot -
Skeletal muscle derived Musclin protects the heart during pathological overload
Authors: M Szaroszyk, B Kattih, A Martin-Gar, FA Trogisch, GM Dittrich, A Grund, A Abouissa, K Derlin, M Meier, T Holler, M Korf-Kling, K Völker, T Garfias Ma, C Pablo Tort, M Boschmann, N Huang, N Froese, C Zwadlo, M Malek Moha, X Luo, M Wagner, J Cordero, R Geffers, S Batkai, T Thum, N Bork, VO Nikolaev, OJ Müller, HA Katus, A El-Armouch, T Kraft, J Springer, G Dobreva, KC Wollert, J Fielitz, S von Haehli, M Kuhn, J Bauersachs, J Heineke
Nature Communications, 2022-01-10;13(1):149.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Cyclo(Val-Pro) and Cyclo(Leu-Hydroxy-Pro) from Pseudomonas sp. (ABS-36) alleviates acute and chronic renal injury under in vitro and in vivo models (Ischemic reperfusion and unilateral ureter obstruction)
Authors: K Hira, P Sharma, A Mahale, O Prakash Ku, A Sajeli Beg
International immunopharmacology, 2021-12-29;103(0):108494.
Species: Mouse, Rat
Sample Types: Cell Lysates
Applications: Western Blot -
The effects of BAFF and APRIL signaling on non-small cell lung cancer cell proliferation and invasiveness
Authors: M Warakomska, M Tynecka, D Lemancewic, K Grubczak, J Dzieciol, M Moniuszko, A Eljaszewic, L Bolkun
Oncology Letters, 2021-08-10;22(4):728.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution
Authors: LG Lima, S Ham, H Shin, EPZ Chai, ESH Lek, RJ Lobb, AF Müller, S Mathivanan, B Yeo, Y Choi, BS Parker, A Möller
Nature Communications, 2021-06-10;12(1):3543.
Species: Human
Sample Types: Exosome Lysates
Applications: Western Blot -
Overexpression of the receptor for advanced glycation end-products in the auditory cortex of rats with noise-induced hearing loss
Authors: CH Lee, KW Kim, DH Lee, SM Lee, SY Kim
Bmc Neuroscience, 2021-05-21;22(1):38.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
Neuropathological and behavioral characterization of aged Grn R493X progranulin-deficient frontotemporal dementia knockin mice
Authors: J Frew, HB Nygaard
Acta neuropathologica communications, 2021-04-01;9(1):57.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot -
Topical nanoparticles interfering with the DNA-LL37 complex to alleviate psoriatic inflammation in mice and monkeys
Authors: Liang H, Yan Y, Wu J et al.
Science Advances
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Interleukin-18 levels and mouse Leydig cell apoptosis during lipopolysaccharide-induced acute inflammatory conditions
Authors: T Inoue, M Aoyama-Ish, M Uemura, H Yamashita, Y Koga, M Terashima, M Usami, J Kotani, J Hirata
J. Reprod. Immunol., 2020-06-20;141(0):103167.
Species: Rat
Sample Types: Cell Culture Lysates
Applications: Western Blot -
Regulation of antitumour CD8 T-cell immunity and checkpoint blockade immunotherapy by Neuropilin-1
Authors: M Leclerc, E Voilin, G Gros, S Corgnac, V de Montpré, P Validire, G Bismuth, F Mami-Choua
Nat Commun, 2019-07-26;10(1):3345.
Species: Human, Mouse
Sample Types: Protein
Applications: Western Blot -
Propranolol regulates ERK1/2 signaling pathway and promotes chronic wound healing in diabetic rats
Authors: X Chang, S Li, XD Xue, F Chang
Eur Rev Med Pharmacol Sci, 2019-05-01;23(10):4498-4506.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
Deletion of Galectin-3 attenuates acute pancreatitis in mice by affecting activation of innate inflammatory cells
Authors: B Stojanovic, I Jovanovic, BS Stojanovic, MD Stojanovic, N Gajovic, G Radosavlje, J Pantic, N Arsenijevi, ML Lukic
Eur. J. Immunol., 2019-04-02;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Cryo-EM structure of the essential ribosome assembly AAA-ATPase Rix7
Authors: YH Lo, M Sobhany, AL Hsu, BL Ford, JM Krahn, MJ Borgnia, RE Stanley
Nat Commun, 2019-01-31;10(1):513.
Applications: Western Blot -
A paradoxical method to enhance compensatory lung growth: Utilizing a VEGF inhibitor
Authors: DT Dao, L Anez-Busti, SS Jabbouri, A Pan, H Kishikawa, PD Mitchell, GL Fell, MA Baker, RS Watnick, H Chen, MS Rogers, DR Bielenberg, M Puder
PLoS ONE, 2018-12-19;13(12):e0208579.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Regulation of vitamin D metabolizing enzymes in murine renal and extrarenal tissues by dietary phosphate, FGF23, and 1,25(OH)2D3
Authors: L Kägi, C Bettoni, EM Pastor-Arr, U Schnitzbau, N Hernando, CA Wagner
PLoS ONE, 2018-05-17;13(5):e0195427.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Fasting selectively blocks development of acute lymphoblastic leukemia via leptin-receptor upregulation
Nat. Med, 2016-12-12;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
The Processed Amino-Terminal Fragment of Human TLR7 Acts as a Chaperone To Direct Human TLR7 into Endosomes.
Authors: Hipp M, Shepherd D, Booth S, Waithe D, Reis E Sousa C, Cerundolo V
J Immunol, 2015-04-27;194(11):5417-25.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Ligation of TLR7 on CD19(+) CD1d(hi) B cells suppresses allergic lung inflammation via regulatory T cells.
Authors: Khan A, Amu S, Saunders S, Hams E, Blackshields G, Leonard M, Weaver C, Sparwasser T, Sheils O, Fallon P
Eur J Immunol, 2015-04-14;45(6):1842-54.
Species: Mouse
Sample Types: Cell Lysates
Applications: Neutralization -
Stage of breast cancer progression influences cellular response to activation of the WNT/planar cell polarity pathway.
Authors: MacMillan, Connor D, Leong, Hon S, Dales, David W, Robertson, Amy E, Lewis, John D, Chambers, Ann F, Tuck, Alan B
Sci Rep, 2014-09-10;4(0):6315.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Highlights of glycosylation and adhesion related genes involved in myogenesis.
Authors: Grassot V, Da Silva A, Saliba J, Maftah A, Dupuy F, Petit J
BMC Genomics, 2014-07-22;15(0):621.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Renal expression of FGF23 and peripheral resistance to elevated FGF23 in rodent models of polycystic kidney disease.
Authors: Spichtig D, Zhang H, Mohebbi N, Pavik I, Petzold K, Stange G, Saleh L, Edenhofer I, Segerer S, Biber J, Jaeger P, Serra A, Wagner C
Kidney Int, 2014-01-08;85(6):1340-50.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
Influence of galectin-9/Tim-3 interaction on herpes simplex virus-1 latency.
Authors: Reddy PB, Sehrawat S, Suryawanshi A
J. Immunol., 2011-10-21;187(11):5745-55.
Applications: Western Blot -
Ocular neovascularization caused by herpes simplex virus type 1 infection results from breakdown of binding between vascular endothelial growth factor A and its soluble receptor.
Authors: Suryawanshi A, Mulik S, Sharma S
J. Immunol., 2011-02-16;186(6):3653-65.
Applications: Western Blot -
Wnt5A Signaling Blocks Progression of Experimental Visceral Leishmaniasis
Authors: Shreyasi Maity, Arijit Chakraborty, Sushil Kumar Mahata, Syamal Roy, Anjan Kumar Das, Malini Sen
Frontiers in Immunology
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Wnt5A Signaling Regulates Gut Bacterial Survival and T Cell Homeostasis
Authors: S Sengupta, S Jati, S Maity, M Sen
mSphere, 2022-12-06;7(6):e0050722.
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Heme Drives Susceptibility of Glomerular Endothelium to Complement Overactivation Due to Inefficient Upregulation of Heme Oxygenase-1
Authors: Olivia May, Nicolas S. Merle, Anne Grunenwald, Viviane Gnemmi, Juliette Leon, Cloé Payet et al.
Frontiers in Immunology
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Anthracycline-free tumor elimination in mice leads to functional and molecular cardiac recovery from cancer-induced alterations in contrast to long-lasting doxorubicin treatment effects
Authors: Stefan Pietzsch, Katharina Wohlan, James T. Thackeray, Maren Heimerl, Sven Schuchardt, Michaela Scherr et al.
Basic Research in Cardiology
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E2A-PBX1 Remodels Oncogenic Signaling Networks in B-cell Precursor Acute Lymphoid Leukemia
Authors: Jesús Duque-Afonso, Chiou-Hong Lin, Kyuho Han, Michael C. Wei, Jue Feng, Jason H. Kurzer et al.
Cancer Research
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The multicellular signalling network of ovarian cancer metastases
Authors: Leah Sommerfeld, Florian Finkernagel, Julia M. Jansen, Uwe Wagner, Andrea Nist, Thorsten Stiewe et al.
Clinical and Translational Medicine
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A standardized blood test for the routine clinical diagnosis of impaired GM-CSF signaling using flow cytometry
Authors: Yoshiomi Kusakabe, Kanji Uchida, Takahiro Hiruma, Yoko Suzuki, Tokie Totsu, Takuji Suzuki et al.
Journal of Immunological Methods
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Pseudomonas aeruginosa Is Associated With Increased Lung Cytokines and Asymmetric Dimethylarginine Compared With Methicillin-Resistant Staphylococcus aureus
Authors: Linda E. Sousse, Collette C. Jonkam, Daniel L. Traber, Hal K. Hawkins, Sebastian W. Rehberg, Lillian D. Traber et al.
Shock
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Lipocalin 2 promotes inflammatory breast cancer tumorigenesis and skin invasion
Authors: Emilly S. Villodre, Xiaoding Hu, Richard Larson, Pascal Finetti, Kristen Gomez, Wintana Balema et al.
Molecular Oncology
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ZBTB7B (ThPOK) IS REQUIRED FOR PATHOGENESIS OF CEREBRAL MALARIA AND PROTECTION AGAINST PULMONARY TUBERCULOSIS
Authors: JM Kennedy, A Georges, AV Bassenden, SM Vidal, AM Berghuis, I Taniuchi, J Majewski, M Lathrop, MA Behr, D Langlais, P Gros
Infect. Immun., 2020-01-22;0(0):.
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Naturally Occurring Variants in LRP1 (Low-Density Lipoprotein Receptor-Related Protein 1) Affect HDL (High-Density Lipoprotein) Metabolism Through ABCA1 (ATP-Binding Cassette A1) and SR-B1 (Scavenger Receptor Class B Type 1) in Humans
Authors: Oldoni F, van Capelleveen JC, Dalila N.
Arteriosclerosis, Thrombosis, and Vascular Biology
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Designing Calcium Release Channel Inhibitors with Enhanced Electron Donor Properties: Stabilizing the Closed State of Ryanodine Receptor Type 1
Authors: Yanping Ye, Daniel Yaeger, Laura J. Owen, Jorge O. Escobedo, Jialu Wang, Jeffrey D. Singer et al.
Molecular Pharmacology
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It works great for western blot
Primary antibody: rat anti-mouse CD45.
Sample: 40 ug and 100 ug cell lysis.