Mouse IgG1 Alexa Fluor® 700-conjugated Antibody

Catalog # Availability Size / Price Qty
IC002N
Detection of Mouse IgG Isotype Control by Flow Cytometry
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Product Details
Citations (2)
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Mouse IgG1 Alexa Fluor® 700-conjugated Antibody Summary

Specificity
Selected for use as mouse IgG1 isotype control for flow cytometry applications.
Source
Monoclonal Mouse IgG1 kappa Clone # 11711
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 5 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Detection of Mouse IgG Control by Flow Cytometry U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human Mer Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB8912N, filled histogram) or Mouse IgG Alexa Fluor® 700-conjugated Isotype Control Antibody (Catalog # IC002N, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG1

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G1
Alternate Names
IgG1; Igh-4; RGD1359539; VH7183

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Citations for Mouse IgG1 Alexa Fluor® 700-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Smooth muscle-derived adventitial progenitor cells promote key cell type transitions controlling plaque stability in atherosclerosis in a Klf4-dependent manner
    Authors: Dubner AM, Lu S, Jolly AJ et al.
    bioRxiv
  2. Oxidized LDL triggers changes in oxidative stress and inflammatory biomarkers in human macrophages
    Authors: OJ Lara-Guzmá, Á Gil-Izquie, S Medina, E Osorio, R Álvarez-Qu, N Zuluaga, C Oger, JM Galano, T Durand, K Muñoz-Dura
    Redox Biol, 2017-11-22;15(0):1-11.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

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