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Mouse IgG2B APC-conjugated Antibody

Catalog #: IC0041A
21 Citations Datasheet / COA / SDS
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IC0041A
Detection of Mouse IgG Isotype Control by Flow Cytometry
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Citations (21)
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Mouse IgG2B APC-conjugated Antibody Summary

Specificity
Selected for use as mouse IgG2B isotype control for flow cytometry applications.
Source
Monoclonal Mouse IgG2B Clone # 133303
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Control Detection of Mouse IgG Isotype Control by Flow Cytometry View Larger

Detection of Mouse IgG Control by Flow Cytometry MDA-MB-231 human breast cancer cell line was stained with Mouse Anti-Human TRAIL R2/TNFRSF10B APC-conjugated Monoclonal Antibody (Catalog # FAB6311A, filled histogram) or Mouse IgG APC-conjugated Isotype Control Antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG2B

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G2B Fc
Alternate Names
IgG2B

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Citations for Mouse IgG2B APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

21 Citations: Showing 1 - 10
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  1. Characterization of the Filovirus-Resistant Cell Line SH-SY5Y Reveals Redundant Role of Cell Surface Entry Factors
    Authors: FJ Zapatero-B, E Dietzel, O Dolnik, K Döhner, R Costa, B Hertel, B Veselkova, J Kirui, A Klintworth, MP Manns, S Pöhlmann, T Pietschman, T Krey, S Ciesek, G Gerold, B Sodeik, S Becker, T von Hahn
    Viruses, 2019-03-19;11(3):.
  2. Senescent mesenchymal stem/stromal cells in pre-metastatic bone marrow of untreated advanced breast cancer patients
    Authors: Francisco Raúl RA贚 BORZONE, María Belén BEL蒒 GIORELLO, Leandro Marcelo MARCELO MARTINEZ, María Cecilia CECILIA SANMARTIN, LEONARDO FELDMAN, FEDERICO DIMASE et al.
    Oncology Research
  3. Cargo and Functional Profile of Saliva-Derived Exosomes Reveal Biomarkers Specific for Head and Neck Cancer
    Authors: Linda Hofmann, Valentin Medyany, Jasmin Ezić, Ramin Lotfi, Beate Niesler, Ralph Röth et al.
    Frontiers in Medicine
  4. Decreased expression of ErbB2 on left ventricular epicardial cells in patients with diabetes mellitus
    Authors: JT de Kay, J Carver, B Shevenell, AM Kosta, S Tsibulniko, E Certo, DB Sawyer, S Ryzhov, MP Robich
    Cellular Signalling, 2022-05-21;96(0):110360.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Impact of Extracellular pH on Apoptotic and Non-Apoptotic TRAIL-Induced Signaling in Pancreatic Ductal Adenocarcinoma Cells
    Authors: Sofie Hagelund, Anna Trauzold
    Frontiers in Cell and Developmental Biology
  6. TGF-beta 1 potentiates V gamma 9Vδ2 T cell adoptive immunotherapy of cancer
    Authors: Richard E. Beatson, Ana C. Parente-Pereira, Leena Halim, Domenico Cozzetto, Caroline Hull, Lynsey M. Whilding et al.
    Cell Reports Medicine
  7. Changes in circulating exosome molecular profiles following surgery/(chemo)radiotherapy: early detection of response in head and neck cancer patients
    Authors: MN Theodoraki, S Laban, EK Jackson, R Lotfi, PJ Schuler, C Brunner, TK Hoffmann, TL Whiteside, L Hofmann
    British Journal of Cancer, 2021-10-12;0(0):.
    Species: Human
    Sample Types: Complex Sample Type
    Applications: Flow Cytometry Control
  8. Modeling cell-specific dynamics and regulation of the common gamma chain cytokines
    Authors: AM Farhat, AC Weiner, C Posner, ZS Kim, B Orcutt-Jah, SM Carlson, AS Meyer
    Cell Reports, 2021-04-27;35(4):109044.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  9. A T-cell–redirecting bispecific G-protein–coupled receptor class 5 member D x CD3 antibody to treat multiple myeloma
    Authors: Kodandaram Pillarisetti, Suzanne Edavettal, Mark Mendonça, Yingzhe Li, Mark Tornetta, Alexander Babich et al.
    Blood
  10. TRAIL Induces Nuclear Translocation and Chromatin Localization of TRAIL Death Receptors
    Authors: U Mert, A Adawy, E Scharff, P Teichmann, A Willms, V Haselmann, C Colmorgen, J Lemke, S von Karste, J Fritsch, A Trauzold
    Cancers (Basel), 2019-08-14;11(8):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Impact of p53 status on TRAIL-mediated apoptotic and non-apoptotic signaling in cancer cells
    Authors: A Willms, H Schittek, S Rahn, J Sosna, U Mert, D Adam, A Trauzold
    PLoS ONE, 2019-04-04;14(4):e0214847.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. NOTCH-mediated non-cell autonomous regulation of chromatin structure during senescence
    Authors: AJ Parry, M Hoare, D Bihary, R Hänsel-Her, S Smith, K Tomimatsu, E Mannion, A Smith, P D'Santos, IA Russell, S Balasubram, H Kimura, SA Samarajiwa, M Narita
    Nat Commun, 2018-05-09;9(1):1840.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Data supporting that adipose-derived mesenchymal stem/stromal cells express angiotensin II receptors in situ and in vitro
    Authors: LV Ageeva, VY Sysoeva, PA Tyurin-Kuz, GV Sharonov, DT Dyikanov, DV Stambolsky, NI Kalinina
    Data Brief, 2017-11-21;16(0):327-333.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Local angiotensin II promotes adipogenic differentiation of human adipose tissue mesenchymal stem cells through type 2 angiotensin receptor
    Authors: VY Sysoeva, LV Ageeva, PA Tyurin-Kuz, GV Sharonov, DT Dyikanov, NI Kalinina, VA Tkachuk
    Stem Cell Res, 2017-11-03;25(0):115-122.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  15. Tumor specific regulatory T cells in the bone marrow of breast cancer patients selectively upregulate the emigration receptor S1P1
    Authors: Anchana Rathinasamy, Christoph Domschke, Yingzi Ge, Hans-Henning Böhm, Steffen Dettling, David Jansen et al.
    Cancer Immunology, Immunotherapy
  16. CXCL10/IP-10 Neutralization Can Ameliorate Lipopolysaccharide-Induced Acute Respiratory Distress Syndrome in Rats
    Authors: Shan Lang, Libing Li, Xuning Wang, Junping Sun, Xinying Xue, Yongjiu Xiao et al.
    PLOS ONE
  17. Lipoxin A4 Attenuates Constitutive and TGF-beta1-Dependent Profibrotic Activity in Human Lung Myofibroblasts.
    Authors: Roach K, Feghali-Bostwick C, Amrani Y, Bradding P
    J Immunol, 2015-08-14;195(6):2852-60.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. NZ28-induced inhibition of HSF1, SP1 and NF-kappa B triggers the loss of the natural killer cell-activating ligands MICA/B on human tumor cells
    Authors: Daniela Schilling, Annett Kühnel, Fabian Tetzlaff, Sarah Konrad, Gabriele Multhoff
    Cancer Immunology, Immunotherapy
  19. Junctional adhesion molecule (JAM)-B supports lymphocyte rolling and adhesion through interaction with alpha4beta1 integrin.
    Authors: Ludwig RJ, Hardt K, Hatting M, Bistrian R, Diehl S, Radeke HH, Podda M, Schon MP, Kaufmann R, Henschler R, Pfeilschifter JM, Santoso S, Boehncke WH
    Immunology, 2009-10-01;128(2):196-205.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Control
  20. Expression of the ULBP ligands for NKG2D by B-NHL cells plays an important role in determining their susceptibility to rituximab-induced ADCC.
    Authors: Inagaki A, Ishida T, Yano H, Ishii T, Kusumoto S, Ito A, Ri M, Mori F, Ding J, Komatsu H, Iida S, Ueda R
    Int. J. Cancer, 2009-07-01;125(1):212-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  21. Chemokine responsiveness of CD4+ CD25+ regulatory and CD4+ CD25- T cells from atopic and nonatopic donors.
    Authors: Ahern D, Lloyd CM, Robinson DS
    Allergy, 2009-02-07;64(8):1121-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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