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Rat IgG2A PE-conjugated Antibody

Catalog #: IC006P
32 Citations 2 Reviews Datasheet / COA / SDS
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Detection of Rat IgG Isotype Control by Flow Cytometry
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Citations (32)
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Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat IgG2A PE-conjugated Antibody Summary

Specificity
Selected for use as rat IgG2A isotype control for flow cytometry applications.
Source
Monoclonal Rat IgG2A Clone # 54447
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Control Detection of Rat IgG Isotype Control by Flow Cytometry View Larger

Detection of Rat IgG Control by Flow Cytometry 4T1 mouse breast cancer cell line was stained with Rat Anti-Mouse EpCAM/TROP-1 PE-conjugated Monoclonal Antibody (Catalog # FAB8998P, filled histogram) or Rat IgG PE-conjugated Isotype Control Antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG2A

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G2A Fc
Alternate Names
IgG2A

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Citations for Rat IgG2A PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

32 Citations: Showing 1 - 10
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  1. Aging-Impaired Filamentous Actin Polymerization Signaling Reduces Alveolar Macrophage Phagocytosis of Bacteria
    Authors: Z Li, Y Jiao, EK Fan, MJ Scott, Y Li, S Li, TR Billiar, MA Wilson, X Shi, J Fan
    J. Immunol., 2017-09-25;0(0):.
  2. TCF-1 controls Treg cell functions that regulate inflammation, CD8+ T cell cytotoxicity and severity of colon cancer
    Authors: A Osman, B Yan, Y Li, KD Pavelko, J Quandt, A Saadalla, MP Singh, M Kazemian, F Gounari, K Khazaie
    Nature Immunology, 2021-08-12;0(0):.
  3. Caffeic Acid Phenethyl Ester Suppresses Oxidative Stress and Regulates M1/M2 Microglia Polarization via Sirt6/Nrf2 Pathway to Mitigate Cognitive Impairment in Aged Mice following Anesthesia and Surgery
    Authors: Yue Wang, Ziwen Cai, Gaofeng Zhan, Xing Li, Shan Li, Xuan Wang et al.
    Antioxidants (Basel)
  4. Impaired differentiation potential of CD34-positive cells derived from mouse hair follicles after long-term culture
    Authors: Y Ouji, M Misu, T Kitamura, D Okuzaki, M Yoshikawa
    Scientific Reports, 2022-06-30;12(1):11011.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Isotype Control
  5. Decreased expression of ErbB2 on left ventricular epicardial cells in patients with diabetes mellitus
    Authors: JT de Kay, J Carver, B Shevenell, AM Kosta, S Tsibulniko, E Certo, DB Sawyer, S Ryzhov, MP Robich
    Cellular Signalling, 2022-05-21;96(0):110360.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. GPA33 is expressed on multiple human blood cell types and distinguishes CD4+ central memory T cells with and without effector function
    Authors: R Opstelten, JS Suwandi, MC Slot, F Morgana, AM Scott, S Laban, T Nikolic, AW Turksma, A Kroeze, C Voermans, JJ Zwaginga, BO Roep, D Amsen
    European Journal of Immunology, 2021-05-04;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: CyTof, Flow Cytometry
  7. Cetuximab-induced natural killer cell cytotoxicity in head and neck squamous cell carcinoma cell lines: investigation of the role of cetuximab sensitivity and HPV status
    Authors: H Baysal, I De Pauw, H Zaryouh, J De Waele, M Peeters, P Pauwels, JB Vermorken, E Smits, F Lardon, J Jacobs, A Wouters
    Br. J. Cancer, 2020-06-16;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Isotype Control
  8. Tissue-Resident PDGFR&alpha+ Progenitor Cells Contribute to Fibrosis versus Healing in a Context- and Spatiotemporally Dependent Manner
    Authors: MP Santini, D Malide, G Hoffman, G Pandey, V D'Escamard, A Nomura-Kit, I Rovira, H Kataoka, J Ochando, RP Harvey, T Finkel, JC Kovacic
    Cell Rep, 2020-01-14;30(2):555-570.e7.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Select gp120 V2 domain specific antibodies derived from HIV and SIV infection and vaccination inhibit gp120 binding to ?4?7
    Authors: S Lertjuthap, C Cicala, D Van Ryk, M Liu, J Yolitz, D Wei, F Nawaz, A Doyle, B Horowitch, C Park, S Lu, Y Lou, S Wang, R Pan, X Jiang, F Villinger, SN Byrareddy, PJ Santangelo, L Morris, CK Wibmer, K Biris, RD Mason, J Gorman, J Hiatt, E Martinelli, M Roederer, D Fujikawa, G Gorini, G Franchini, A Arakelyan, AA Ansari, K Pattanapan, XP Kong, AS Fauci, J Arthos
    PLoS Pathog., 2018-08-28;14(8):e1007278.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  10. Simultaneous targeting of EGFR, HER2 and HER4 by afatinib overcomes intrinsic and acquired cetuximab resistance in head and neck squamous cell carcinoma cell lines
    Authors: I De Pauw, F Lardon, J Van den Bo, H Baysal, E Fransen, V Deschoolme, P Pauwels, M Peeters, JB Vermorken, A Wouters
    Mol Oncol, 2018-05-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Axl inhibition induces the antitumor immune response which can be further potentiated by PD-1 blockade in the mouse cancer models
    Authors: Zhiqiang Guo, Yan Li, Dandan Zhang, Jiaying Ma
    Oncotarget
  12. HIV-related proteins prolong macrophage survival through induction of Triggering receptor expressed on myeloid cells-1
    Authors: Z Yuan, X Fan, B Staitieh, C Bedi, P Spearman, DM Guidot, RT Sadikot
    Sci Rep, 2017-02-09;7(0):42028.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. During acute experimental infection with the reticulotropic Trypanosoma cruzi strain Tulahuen IL-22 is induced IL-23-dependently but is dispensable for protection
    Sci Rep, 2016-09-21;6(0):32927.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. CC chemokine receptor 10 cell surface presentation in melanocytes is regulated by the novel interaction partner S100A10
    Authors: F. Hessner, C. P. Dlugos, T. Chehab, C. Schaefer, B. Homey, V. Gerke et al.
    Scientific Reports
  15. Expression of the galectin-9-Tim-3 pathway in glioma tissues is associated with the clinical manifestations of glioma
    Authors: ZENGJIN LIU, HUAMIN HAN, XIN HE, SHOUWEI LI, CHENXING WU, CHUNJIANG YU et al.
    Oncology Letters
  16. Hepatitis C Virus Activates a Neuregulin-Driven Circuit to Modify Surface Expression of Growth Factor Receptors of the ErbB Family
    Authors: Sabine Stindt, Patricia Cebula, Ute Albrecht, Verena Keitel, Jan Schulte am Esch, Wolfram T. Knoefel et al.
    PLOS ONE
  17. CXCR3 as a molecular target in breast cancer metastasis: inhibition of tumor cell migration and promotion of host anti-tumor immunity
    Authors: Guiquan Zhu, H. Hannah Yan, Yanli Pang, Jiang Jian, Bhagelu R. Achyut, Xinhua Liang et al.
    Oncotarget
  18. Oral supplementation of diabetic mice with propolis restores the proliferation capacity and chemotaxis of B and T lymphocytes towards CCL21 and CXCL12 by modulating the lipid profile, the pro-inflammatory cytokine levels and oxidative stress.
    Authors: Al Ghamdi A, Badr G, Hozzein W, Allam A, Al-Waili N, Al-Wadaan M, Garraud O
    BMC Immunol, 2015-09-15;16(0):54.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  19. Partial maintenance and long-term expansion of murine skin epithelial stem cells by Wnt-3a in vitro.
    Authors: Ouji Y, Ishizaka S, Nakamura-Uchiyama F, Okuzaki D, Yoshikawa M
    J Invest Dermatol, 2014-12-01;135(6):1598-608.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Control
  20. Triggering receptor expressed on myeloid cells 1 (TREM-1)-mediated Bcl-2 induction prolongs macrophage survival.
    Authors: Yuan Z, Syed M, Panchal D, Joo M, Colonna M, Brantly M, Sadikot R
    J Biol Chem, 2014-04-07;289(21):15118-29.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. Impaired B cell receptor signaling is responsible for reduced TACI expression and function in X-linked immunodeficient mice.
    Authors: Uslu K, Coleman A, Allman W, Katsenelson N, Bram R, Alugupalli K, Akkoyunlu M
    J Immunol, 2014-03-19;192(8):3582-95.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. Intracellular coexpression of CXC- and CC- chemokine receptors and their ligands in human melanoma cell lines and dynamic variations after xenotransplantation.
    Authors: Pinto S, Martinez-Romero A, O'Connor J, Gil-Benso R, San-Miguel T, Terradez L, Monteagudo C, Callaghan R
    BMC Cancer, 2014-02-22;14(0):118.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. Gamma-tocotrienol inhibits lipopolysaccharide-induced interlukin-6 and granulocyte-colony stimulating factor by suppressing C/EBP-beta and NF-kappa B in macrophages
    Authors: Yun Wang, Qing Jiang
    The Journal of Nutritional Biochemistry
  24. Upregulation of the tim-3/galectin-9 pathway of T cell exhaustion in chronic hepatitis B virus infection.
    Authors: Nebbia G, Peppa D, Schurich A, Khanna P, Singh H, Cheng Y, Rosenberg W, Dusheiko G, Gilson R, Chinaleong J, Kennedy P, Maini M
    PLoS ONE, 2012-10-24;7(10):e47648.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  25. Targeting Stat3 induces senescence in tumor cells and elicits prophylactic and therapeutic immune responses against breast cancer growth mediated by NK cells and CD4+ T cells.
    J. Immunol., 2012-06-29;189(3):1162-72.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Control
  26. Steady-state generation of mucosal IgA+ plasmablasts is not abrogated by B-cell depletion therapy with rituximab.
    Authors: Mei HE, Frolich D, Giesecke C, Loddenkemper C, Reiter K, Schmidt S, Feist E, Daridon C, Tony HP, Radbruch A, Dorner T
    Blood, 2010-09-09;116(24):5181-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  27. Lipid-cytokine-chemokine cascade drives neutrophil recruitment in a murine model of inflammatory arthritis.
    Authors: Chou RC, Kim ND, Sadik CD
    Immunity, 2010-08-27;33(2):266-78.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Control
  28. Murine trophoblast cells induce NK cell interferon-gamma production through KLRK1.
    Authors: Carayannopoulos LN, Barks JL, Yokoyama WM
    Biol. Reprod., 2010-05-19;83(3):404-14.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Control
  29. Chemokine responsiveness of CD4+ CD25+ regulatory and CD4+ CD25- T cells from atopic and nonatopic donors.
    Authors: Ahern D, Lloyd CM, Robinson DS
    Allergy, 2009-02-07;64(8):1121-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  30. FcgammaRIIB signals inhibit BLyS signaling and BCR-mediated BLyS receptor up-regulation.
    Authors: Crowley JE, Stadanlick JE, Cambier JC, Cancro MP
    Blood, 2008-09-12;113(7):1464-73.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Control
  31. TREM-1 promotes survival during septic shock in mice.
    Authors: Gibot S, Massin F, Marcou M, Taylor V, Stidwill R, Wilson P, Singer M, Bellingan G
    Eur. J. Immunol., 2007-02-01;37(2):456-66.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Control
  32. Characterization of the human chemerin receptor--ChemR23/CMKLR1--as co-receptor for human and simian immunodeficiency virus infection, and identification of virus-binding receptor domains.
    Authors: Martensson UE, Fenyo EM, Olde B, Owman C
    Virology, 2006-08-10;355(1):6-17.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control

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Reviews for Rat IgG2A PE-conjugated Antibody

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Rat IgG2A PE-conjugated Antibody
By Tahmineh Safaie on 12/23/2019
Application: Flow Sample Tested: 4T1 mouse breast cancer cell line Species: Mouse
Flow Cytometry Rat IgG2A PE-conjugated Antibody IC006P
Rat IgG2A PE-conjugated Antibody
By Anonymous on 09/08/2016
Application: Flow Sample Tested: Bone marrow-derived macrophages Species: Mouse
Flow Cytometry Rat IgG2A PE-conjugated Antibody IC006P