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Rat IgG2B PE-conjugated Antibody

Catalog #: IC013P
8 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
IC013P
Detection of Rat IgG Isotype Control by Flow Cytometry
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Citations (8)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat IgG2B PE-conjugated Antibody Summary

Specificity
Selected for use as a rat IgG2B isotype control for flow cytometry applications.
Source
Monoclonal Rat IgG2B Clone # 141945
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Control Detection of Rat IgG Isotype Control by Flow Cytometry View Larger

Detection of Rat IgG Control by Flow Cytometry Human peripheral blood mononuclear cells (PBMCs) were stained with Rat Anti-Human/Mouse CD44 PE-conjugated Monoclonal Antibody (Catalog # FAB6127P, filled histogram) or Rat IgG PE-conjugated Isotype Control Antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG2B

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G2B Fc
Alternate Names
IgG2B

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Citations for Rat IgG2B PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. CCR2 of Tumor Microenvironmental Cells Is a Relevant Modulator of Glioma Biology
    Authors: M Felsenstei, A Blank, AD Bungert, A Mueller, A Ghori, I Kremenetsk, O Rung, T Broggini, K Turkowski, L Scherschin, J Raggatz, P Vajkoczy, S Brandenbur
    Cancers (Basel), 2020-07-13;12(7):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Dual‑targeting nanomicelles with CD133 and CD44 aptamers for enhanced delivery of gefitinib to two populations of lung cancer‑initiating cells
    Authors: Xiaolong Huang, Jun Wan, Dewen Leng, Yingying Zhang, Shuo Yang
    Experimental and Therapeutic Medicine
  3. Longitudinal changes in the expression of IL-33 and IL-33 regulated genes in relapsing remitting MS
    Authors: S Sriram, G Shaginurov, JT Tossberg, C Natarajan, CF Spurlock, TM Aune
    PLoS ONE, 2018-12-18;13(12):e0208755.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Glatiramer Acetate, Dimethyl Fumarate, and Monomethyl Fumarate Upregulate the Expression of CCR10 on the Surface of Natural Killer Cells and Enhance Their Chemotaxis and Cytotoxicity
    Authors: Zaidoon Al-Jaderi
    Front Immunol, 2016-10-19;7(0):437.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. AMPK-Activated Protein Kinase Suppresses Ccr2 Expression by Inhibiting the NF-kappaB Pathway in RAW264.7 Macrophages.
    Authors: Kumase F, Takeuchi K, Morizane Y, Suzuki J, Matsumoto H, Kataoka K, Al-Moujahed A, Maidana D, Miller J, Vavvas D
    PLoS ONE, 2016-01-22;11(1):e0147279.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  6. CX3CR1-dependent renal macrophage survival promotes Candida control and host survival
    Authors: Michail S. Lionakis, Muthulekha Swamydas, Brett G. Fischer, Theo S. Plantinga, Melissa D. Johnson, Martin Jaeger et al.
    Journal of Clinical Investigation
  7. Chemokine receptor CX3CR1 contributes to macrophage survival in tumor metastasis.
    Authors: Zheng, Jiao, Yang, Min, Shao, Jianghua, Miao, Yanju, Han, Jiahuai, Du, Jie
    Mol Cancer, 2013-11-18;12(1):141.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  8. Chemokine Receptor Ccr1 Drives Neutrophil-Mediated Kidney Immunopathology and Mortality in Invasive Candidiasis
    Authors: Michail S. Lionakis, Brett G. Fischer, Jean K. Lim, Muthulekha Swamydas, Wuzhou Wan, Chyi-Chia Richard Lee et al.
    PLoS Pathogens

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