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Goat IgG APC-conjugated Antibody

Catalog #: IC108A
14 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
IC108A
Detection of Goat IgG Isotype Control by Flow Cytometry
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Product Details
Citations (14)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Goat IgG APC-conjugated Antibody Summary

Specificity
Serum was obtained from naive (non-immunized) goats.
Source
Polyclonal Goat IgG
Purification
Protein A or G purified
Immunogen
NA
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies for flow cytometry applications. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems specific polyclonal antibodies. The recommended concentration is 10 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Control Detection of Goat IgG Isotype Control by Flow Cytometry View Larger

Detection of Goat IgG Control by Flow Cytometry J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Goat Anti-Mouse CL-P1/COLEC12 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB3130A, filled histogram) or Goat IgG APC-conjugated Isotype Control Antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

Product Datasheets

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Citations for Goat IgG APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Type I interferon signaling induces melanoma cell-intrinsic PD-1 and its inhibition antagonizes immune checkpoint blockade
    Authors: Holzgruber, J;Martins, C;Kulcsar, Z;Duplaine, A;Rasbach, E;Migayron, L;Singh, P;Statham, E;Landsberg, J;Boniface, K;Seneschal, J;Hoetzenecker, W;Berdan, EL;Ho Sui, S;Ramsey, MR;Barthel, SR;Schatton, T;
    Nature communications
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Regulation of PD-L1 expression in non–small cell lung cancer by interleukin-1 beta
    Authors: Aiko Hirayama, Kentaro Tanaka, Hirono Tsutsumi, Takayuki Nakanishi, Sho Yamashita, Shun Mizusaki et al.
    Frontiers in Immunology
  3. Caffeic Acid Phenethyl Ester Suppresses Oxidative Stress and Regulates M1/M2 Microglia Polarization via Sirt6/Nrf2 Pathway to Mitigate Cognitive Impairment in Aged Mice following Anesthesia and Surgery
    Authors: Yue Wang, Ziwen Cai, Gaofeng Zhan, Xing Li, Shan Li, Xuan Wang et al.
    Antioxidants (Basel)
  4. Highly efficacious and safe neutralizing DNA aptamer of SARS-CoV-2 as an emerging therapy for COVID-19 disease
    Authors: Mohamad Ammar Ayass, Trivendra Tripathi, Natalya Griko, Victor Pashkov, Jun Dai, Jin Zhang et al.
    Virology Journal
  5. Fungal sensing by dectin-1 directs the non-pathogenic polarization of TH17 cells through balanced type I IFN responses in human DCs
    Authors: SI Gringhuis, TM Kaptein, EBM Remmerswaa, A Drewniak, BA Wevers, B Theelen, GRAM D'Haens, T Boekhout, TBH Geijtenbee
    Nature Immunology, 2022-12-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. CLN7/MFSD8 may be an important factor for SARS-CoV-2 cell entry
    Authors: Elena-Sofia Heinl, Sebastian Lorenz, Barbara Schmidt, Nouf Nasser M Laqtom, Joseph R. Mazzulli, Laetitia Francelle et al.
    iScience
  7. The Cell Surface Heparan Sulfate Proteoglycan Syndecan-3 Promotes Ovarian Cancer Pathogenesis
    Authors: L Hillemeyer, NA Espinoza-S, B Greve, N Hassan, A Chelariu-R, L Kiesel, M Götte
    International Journal of Molecular Sciences, 2022-05-21;23(10):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Isotype Control
  8. SMPDL3b modulates insulin receptor signaling in diabetic kidney disease
    Authors: A Mitrofanov, SK Mallela, GM Ducasa, TH Yoo, E Rosenfeld-, ID Zelnik, J Molina, J Varona San, M Ge, A Sloan, JJ Kim, C Pedigo, J Bryn, I Volosenco, C Faul, YH Zeidan, C Garcia Her, AJ Mendez, I Leibiger, GW Burke, AH Futerman, L Barisoni, Y Ishimoto, R Inagi, S Merscher, A Fornoni
    Nat Commun, 2019-06-19;10(1):2692.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Regulated Delayed Shigella flexneri 2a O-antigen Synthesis in Live Recombinant Salmonella enterica Serovar Typhimurium Induces Comparable Levels of Protective Immune Responses with Constitutive Antigen Synthesis System
    Authors: H Su, Q Liu, S Wang, R Curtiss, Q Kong
    Theranostics, 2019-05-26;9(12):3565-3579.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Fine tuning the extracellular environment accelerates the derivation of kidney organoids from human pluripotent stem cells
    Authors: Garreta E, Prado P, Tarantino C et al.
    Nature Materials
  11. ??CD4effector/effector memory T cells differentiate into productively and latently infected central memory T cells by TGF?1 during HIV-1 infection
    Authors: KW Cheung, T Wu, SF Ho, YC Wong, L Liu, H Wang, Z Chen
    J. Virol., 2018-03-28;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Mesendogen, a novel inhibitor of TRPM6, promotes mesoderm and definitive endoderm differentiation of human embryonic stem cells through alteration of magnesium homeostasis
    Authors: Yijie Geng, Bradley Feng
    Heliyon
  13. MicroRNAs are implicated in the suppression of CD4+CD25- conventional T cell proliferation by CD4+CD25+ regulatory T cells.
    Authors: Zhou S, Dong X, Zhang C, Chen X, Zhu J, Li W, Song X, Xu Z, Zhang W, Yang X, Li Y, Liu F, Sun C
    Mol Immunol, 2015-02-01;63(2):464-72.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  14. Angiotensin II-dependent TGF-beta signaling contributes to Loeys-Dietz syndrome vascular pathogenesis.
    Authors: Gallo E, Loch D, Habashi J, Calderon J, Chen Y, Bedja D, van Erp C, Gerber E, Parker S, Sauls K, Judge D, Cooke S, Lindsay M, Rouf R, Myers L, ap Rhys C, Kent K, Norris R, Huso D, Dietz H
    J Clin Invest, 2013-12-20;124(1):448-60.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control

FAQs

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Reviews for Goat IgG APC-conjugated Antibody

Average Rating: 4.5 (Based on 2 Reviews)

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Goat IgG APC-conjugated Antibody
By Anonymous on 02/02/2023
Application: Flow Sample Tested: NK-92 human natural killer lymphoma cell line Species: Human

Use as Iso-type Ctrl

Flow Cytometry Goat IgG APC-conjugated Antibody IC108A
Goat IgG APC-conjugated Antibody
By Anonymous on 08/24/2017
Application: Flow Sample Tested: Adult splenocytes Species: Mouse

Isotype control