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Mouse IgG2A Isotype Control

Catalog #: MAB003
97 Citations 7 Reviews Datasheet / COA / SDS
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MAB003
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Detection of Mouse IgG Isotype Control by Flow Cytometry
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Citations (97)
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Reviews (7)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IgG2A Isotype Control Summary

Specificity
Selected for use as mouse IgG2A isotype control. In direct ELISAs, does not cross-react with recombinant human (rh) 6Ckine, recombinant mouse (rm) 6Ckine, rmBLC, rmC10, recombinant rat (rr) CINC-1, rrCINC-2 alpha, rrCINC-2 beta, rrCINC-3, rhCK beta 8-1, recombinant viral (rv) CMV UL146 T23, rmCRG-2, rhCXC-X, rhDendrokine, rhENA-78, rhEotaxin, rmEotaxin, rhEotaxin-2, rmEotaxin-2, rhEotaxin-3, rhFractalkine, rmFractalkine, rrFractalkine, rhGCP-2, rmGCP-2, rhGRO alpha, rhGRO beta, rhGRO gamma, rhHCC-1, rhHCC-4, rhI-309, rhIL-8, recombinant porcine IL-8, rhIP-10, rhI-TAC, rmJE, rmKC, rhLeukotactin-1, rhLymphotactin, rmLymphotactin, rmMARC, rhMCP-1, rhMCP-2, rhMCP-3, rhMCP-4, rmMCP-5, rvMCV Type 2, rhMDC, rmMDC, rhMIG, rmMIG, rhMIP-1 alpha, rmMIP-1 alpha, rvMIP-1 alpha, rhMIP-1 beta, rmMIP-1 beta, rvMIP-1 beta, rmMIP-1 gamma, rmMIP-1δ, rvMIP-1 gamma, rmMIP-2, rhMIP-3 alpha, rmMIP-3 alpha, rrMIP-3 alpha, rhMIP-3 beta, rmMIP-3 beta, rhMPIF-1, rhNAP-2, rhParc, rhRANTES, rmRANTES, rhSDF-1 alpha, rmSDF-1 alpha, rhSDF-1 beta, rhTARC, rmTARC, rmTCA-3, rhTeck, or rmTeck.
Source
Monoclonal Mouse IgG2A Clone # 20102
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. Use at the same concentration as the detection antibody.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Control Detection of Mouse IgG Isotype Control by Flow Cytometry View Larger

Detection of Mouse IgG Control by Flow Cytometry Human peripheral blood granulocytes were stained with Mouse Anti-Human CXCR1/IL-8 RA Monoclonal Antibody (Catalog # MAB330, filled histogram) or Mouse IgG Isotype Control Antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Immunohistochemistry Detection of Mouse IgG2a by Immunohistochemistry View Larger

Detection of Mouse IgG2a by Immunohistochemistry Transmammary transfer of BMP9 and BMP10 blocking Abs leads to abnormal hypervascularization in neonatal retinas.(A–C”) Representative images of fluorescent isolectin B4-stained retinas either from P6 neonates fed for 3 days by dams injected on P3 with PBS (A), control IgG2a/b Abs (B), or BMP9/10 blocking Abs (C–C”). (C’,C”) are higher magnification images of the relevant boxed areas in C. a, artery; v, vein; scale bars, 500 μm. (D–L) Higher magnification showing retinal vasculature fields between an artery and a vein (Plexus, D–F), or at the front of an artery (G–I) or a vein (J–L) from neonates treated as in (A–C). Scale bars, 100 μm. (M–O) Scatter plots showing the vascular density on retinal ‘petals’ at the plexus (M), artery front (N), or vein front (O). Data represent mean ± s.e.m. (n = 6 pups per group from 2 dams); ****P < 0.0001, ANOVA and Kruskal-Wallis test. Arrows indicate AVMs, defined as direct shunts between an artery and a vein. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep37366), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgG2A

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G2A Fc
Alternate Names
IgG2A

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Citations for Mouse IgG2A Isotype Control

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

97 Citations: Showing 1 - 10
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  1. DNA methyltransferase 3 alpha and TET methylcytosine dioxygenase 2 restrain mitochondrial DNA-mediated interferon signaling in macrophages
    Authors: Cobo I, Tanaka TN, Chandra Mangalhara K et al.
    Immunity
  2. The interactome of metabolic enzyme carbonic anhydrase IX reveals novel roles in tumor cell migration and invadopodia/MMP14-mediated invasion
    Authors: M Swayampaku, PC McDonald, M Vallejo, E Coyaud, SC Chafe, A Westerback, G Venkateswa, J Shankar, G Gao, EMN Laurent, Y Lou, KL Bennewith, CT Supuran, IR Nabi, B Raught, S Dedhar
    Oncogene, 2017-07-10;36(45):6244-6261.
  3. Interleukin-4 Receptor Inhibition Targeting Metastasis Independent of Macrophages
    Authors: MM Cleary, N Bharathy, J Abraham, ER Rudzinski, JE Michalek, C Keller
    Molecular Cancer Therapeutics, 2021-04-14;0(0):.
  4. The Heparin-Binding Hemagglutinin of Nocardia cyriacigeorgica GUH-2 Stimulates Inflammatory Cytokine Secretion Through Activation of Nuclear Factor kappa B and Mitogen-Activated Protein Kinase Pathways via TLR4
    Authors: Ji X, Zhang X et al
    Front Cell Infect Microbiol
  5. Mucosal-associated invariant T cells contribute to suppression of inflammatory myeloid cells in immune-mediated kidney disease
    Authors: Gnirck, AC;Philipp, MS;Waterhölter, A;Wunderlich, M;Shaikh, N;Adamiak, V;Henneken, L;Kautz, T;Xiong, T;Klaus, D;Tomczyk, P;Al-Bahra, MM;Menche, D;Walkenhorst, M;Lantz, O;Willing, A;Friese, MA;Huber, TB;Krebs, CF;Panzer, U;Kurts, C;Turner, JE;
    Nature communications
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  6. Lactobacillus from the Altered Schaedler Flora maintain IFN? homeostasis to promote behavioral stress resilience
    Authors: Merchak, AR;Wachamo, S;Brown, LC;Thakur, A;Moreau, B;Brown, RM;Rivet-Noor, C;Raghavan, T;Gaultier, A;
    Brain, behavior, and immunity
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  7. The pro-inflammatory response to influenza A virus infection is fueled by endothelial cells
    Authors: Lisa Bauer, Laurine C Rijsbergen, Lonneke Leijten, Feline FW Benavides, Danny Noack, Mart M Lamers et al.
    Life Science Alliance
  8. Antitumor effects of NK cells expanded by activation pre?processing of autologous feeder cells before irradiation in colorectal cancer
    Authors: Koh, EK;Lee, HR;Son, WC;Park, GY;Bae, J;Park, YS;
    Oncology letters
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. PhosY-secretome profiling combined with kinase-substrate interaction screening defines active c-Src-driven extracellular signaling
    Authors: Backe, SJ;Votra, SD;Stokes, MP;Sebesty�n, E;Castelli, M;Torielli, L;Colombo, G;Woodford, MR;Mollapour, M;Bourboulia, D;
    Cell reports
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization Control
  10. Pericyte stem cells induce Ly6G + cell accumulation and immunotherapy resistance in pancreatic cancer
    Authors: Zhichong Wu, Kevin Thierry, Sophie Bachy, Xinyi Zhang, Pia Gamradt, Hector Hernandez‐Vargas et al.
    EMBO reports
  11. CCR7 Mediates Cell Invasion and Migration in Extrahepatic Cholangiocarcinoma by Inducing Epithelial-Mesenchymal Transition
    Authors: M Oba, Y Nakanishi, T Mitsuhashi, K Sasaki, KC Hatanaka, M Sasaki, A Nange, A Okumura, M Hayashi, Y Yoshida, T Nitta, T Ueno, T Yamada, M Ono, S Kuwabara, K Okamura, T Tsuchikawa, T Nakamura, T Noji, T Asano, K Tanaka, K Takayama, Y Hatanaka, S Hirano
    Cancers, 2023-03-21;15(6):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Isotype Control
  12. Inositol pyrophosphate profiling reveals regulatory roles of IP6K2-dependent enhanced IP7 metabolism in the enteric nervous system
    Authors: Masatoshi Ito, Natsuko Fujii, Saori Kohara, Shuho Hori, Masayuki Tanaka, Christopher Wittwer et al.
    Journal of Biological Chemistry
  13. Spontaneous Osteoclastogenesis, a risk factor for bone metastasis in advanced luminal A-type breast cancer patients
    Authors: Valeria Fernández Vallone, Francisco Raúl Borzone, Leandro Marcelo Martinez, María Belén Giorello, Hosoon Choi, Federico Dimase et al.
    Frontiers in Oncology
  14. FSAP Protects against Histone-Mediated Increase in Endothelial Permeability In Vitro
    Authors: XY Cui, B Stavik, B Thiede, PM Sandset, SM Kanse
    International Journal of Molecular Sciences, 2022-11-08;23(22):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization Control
  15. A novel aquaporin-4-associated optic neuritis rat model with severe pathological and functional manifestations
    Authors: Y Morita, T Itokazu, T Nakanishi, SI Hiraga, T Yamashita
    Journal of Neuroinflammation, 2022-10-27;19(1):263.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  16. Engineering of immune checkpoints B7-H3 and CD155 enhances immune compatibility of MHC-I-/- iPSCs for beta cell replacement
    Authors: R Chimienti, T Baccega, S Torchio, F Manenti, S Pellegrini, A Cospito, A Amabile, MT Lombardo, P Monti, V Sordi, A Lombardo, M Malnati, L Piemonti
    Cell Reports, 2022-09-27;40(13):111423.
    Species: Mouse
    Sample Types: In Vivo
  17. Comparison between intratumoral and intravenously administered oncolytic virus therapy with Newcastle disease virus in a xenograft murine model for pancreatic adenocarcinoma
    Authors: JF de Graaf, M Huberts, D Groeneveld, S van Nieuwk, CHJ van Eijck, RAM Fouchier, BG van den Ho
    Heliyon, 2022-07-09;8(7):e09915.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Isotype Control
  18. A JAM-A–tetraspanin– alpha v beta 5 integrin complex regulates contact inhibition of locomotion
    Authors: Daniel Kummer, Tim Steinbacher, Sonja Thölmann, Mariel Flavia Schwietzer, Christian Hartmann, Simone Horenkamp et al.
    Journal of Cell Biology
  19. Reduced Replication of Highly Pathogenic Avian Influenza Virus in Duck Endothelial Cells Compared to Chicken Endothelial Cells Is Associated with Stronger Antiviral Responses
    Authors: ACM de Bruin, MI Spronken, TM Bestebroer, RAM Fouchier, M Richard
    Viruses, 2022-01-17;14(1):.
    Species: Duck
    Sample Types: Whole Tissue
    Applications: IHC Control
  20. Hyperinflammatory environment drives dysfunctional myeloid cell effector response to bacterial challenge in COVID-19
    Authors: S Mairpady S, A Gómez-Meji, TA Schweizer, M Huemer, CC Chang, C Acevedo, J Bergada-Pi, C Vulin, DA Hofmaenner, TC Scheier, S Hertegonne, E Parietti, N Miroshniko, PD Wendel Gar, MP Hilty, PK Buehler, RA Schuepbach, SD Brugger, AS Zinkernage
    PloS Pathogens, 2022-01-10;18(1):e1010176.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization Control
  21. Interleukin-17 affects synaptic plasticity and cognition in an experimental model of multiple sclerosis
    Authors: M Di Filippo, A Mancini, L Bellingacc, L Gaetani, P Mazzocchet, T Zelante, L La Barbera, A De Luca, M Tantucci, A Tozzi, V Durante, M Sciaccalug, A Megaro, D Chiasserin, N Salvadori, V Lisetti, E Portaccio, C Costa, P Sarchielli, MP Amato, L Parnetti, MT Viscomi, L Romani, P Calabresi
    Cell Reports, 2021-12-07;37(10):110094.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Electrophysiology
  22. The Laminin Receptors Basal Cell Adhesion Molecule/Lutheran and Integrin alpha 7 beta 1 on Human Hematopoietic Stem Cells
    Authors: Parimala Sonika Godavarthy, Christina B. Walter, Claudia Lengerke, Gerd Klein
    Frontiers in Cell and Developmental Biology
  23. The ammonia oxidizing bacterium Nitrosomonas eutropha blocks T helper 2 cell polarization via the anti-inflammatory cytokine IL-10
    Authors: D Maura, N Elmekki, CA Goddard
    Scientific Reports, 2021-07-08;11(1):14162.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization Control
  24. Comprehensive mutagenesis identifies the peptide repertoire of a p53 T-cell receptor mimic antibody that displays no toxicity in mice transgenic for human HLA-A*0201
    Authors: I Trenevska, AP Anderson, C Bentley, T Hassanali, S Wiblin, S Maguire, F Pezzella, AH Banham, D Li
    PLoS ONE, 2021-04-09;16(4):e0249967.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  25. Helicobacter pylori-induced Rev-erb&alpha fosters gastric bacteria colonization by impairing host innate and adaptive defense
    Authors: FY Mao, YP Lv, CJ Hao, YS Teng, YG Liu, P Cheng, SM Yang, W Chen, T Liu, QM Zou, R Xie, JY Xu, Y Zhuang
    Cellular and Molecular Gastroenterology and Hepatology, 2021-03-03;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  26. The immunity of the recombinant prokaryotic and eukaryotic subunit vaccines against cutaneous leishmaniasis
    Authors: S Salari, I Sharifi, M Bamorovat, P Ghasemi Ne
    Microbial pathogenesis, 2021-02-18;153(0):104807.
    Species: Mouse
    Sample Types: Serum
    Applications: Isotype Control
  27. Induction of the IL-1RII decoy receptor by NFAT/FOXP3 blocks IL-1 beta -dependent response of Th17 cells
    Authors: Dong Hyun Kim, Hee Young Kim, Sunjung Cho, Su-Jin Yoo, Won-Ju Kim, Hye Ran Yeon et al.
    eLife
  28. ILT4 inhibition prevents TAM- and dysfunctional T cell-mediated immunosuppression and enhances the efficacy of anti-PD-L1 therapy in NSCLC with EGFR activation
    Authors: X Chen, A Gao, F Zhang, Z Yang, S Wang, Y Fang, J Li, J Wang, W Shi, L Wang, Y Zheng, Y Sun
    Theranostics, 2021-01-19;11(7):3392-3416.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  29. Inhibition of interferon-signalling halts cancer-associated fibroblast-dependent protection of breast cancer cells from chemotherapy
    Authors: RV Broad, SJ Jones, MC Teske, LM Wastall, AM Hanby, JL Thorne, TA Hughes
    British Journal of Cancer, 2021-01-04;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Isotype Control
  30. Regeneration of the pulmonary vascular endothelium after viral pneumonia requires COUP-TF2
    Authors: Gan Zhao, Aaron I. Weiner, Katherine M. Neupauer, Maria Fernanda de Mello Costa, Gargi Palashikar, Stephanie Adams-Tzivelekidis et al.
    Science Advances
  31. IL35 predicts prognosis in gastric cancer and is associated with angiogenesis by altering TIMP1, PAI1, and IGFBP1
    Authors: X Li, N Niu, J Sun, Y Mou, X He, L Mei
    FEBS Open Bio, 2020-11-09;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization Control
  32. T cell-intrinsic role for Nod2 in protection against Th17-mediated uveitis
    Authors: RJ Napier, EJ Lee, MP Davey, EE Vance, JM Furtado, PE Snow, KA Samson, SJ Lashley, BR Brown, R Horai, MJ Mattapalli, B Xu, MC Callegan, LS Uebelhoer, CL Lancioni, RK Vehe, BA Binstadt, JR Smith, RR Caspi, HL Rosenzweig
    Nat Commun, 2020-10-26;11(1):5406.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  33. TLR4-Mediated Pathway Triggers Interferon-Independent G0 Arrest and Antiviral SAMHD1 Activity in Macrophages
    Authors: Petra Mlcochova, Helena Winstone, Lorena Zuliani-Alvarez, Ravindra K. Gupta
    Cell Reports
  34. Nfa34810 Facilitates Nocardia farcinica Invasion of Host Cells and Stimulates Tumor Necrosis Factor Alpha Secretion through Activation of the NF-&kappaB and Mitogen-Activated Protein Kinase Pathways via Toll-Like Receptor 4
    Authors: X Ji, X Zhang, H Li, L Sun, X Hou, H Song, L Han, S Xu, X Qiu, X Wang, N Zheng, Z Li
    Infect. Immun., 2020-03-23;88(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization Control
  35. Type I Interferons Suppress Anti-parasitic Immunity and Can Be Targeted to Improve Treatment of Visceral Leishmaniasis
    Authors: R Kumar, PT Bunn, SS Singh, SS Ng, M Montes de, F De Labasti, SB Chauhan, N Singh, RJ Faleiro, CL Edwards, TCM Frame, M Sheel, RJ Austin, SW Lane, T Bald, MJ Smyth, GR Hill, SE Best, A Haque, D Corvino, N Waddell, L Koufarioti, P Mukhopadha, M Rai, J Chakravart, OP Singh, D Sacks, S Nylen, J Uzonna, S Sundar, CR Engwerda
    Cell Rep, 2020-02-25;30(8):2512-2525.e9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization Control
  36. Proteome Profiling Uncovers an Autoimmune Response Signature That Reflects Ovarian Cancer Pathogenesis
    Authors: M Kobayashi, H Katayama, E Irajizad, JV Vykoukal, JF Fahrmann, DL Kundnani, CY Yu, Y Cai, FC Hsiao, WL Yang, Z Lu, J Celestino, JP Long, KA Do, KH Lu, JJ Ladd, N Urban, RC Bast, SM Hanash
    Cancers (Basel), 2020-02-19;12(2):.
    Species: Mouse
    Sample Types: Cell Culture Lysates
    Applications: Immunoprecipitation
  37. Influenza A viruses are transmitted via the air from the nasal respiratory epithelium of ferrets
    Authors: M Richard, JMA van den Br, TM Bestebroer, P Lexmond, D de Meulder, RAM Fouchier, AC Lowen, S Herfst
    Nat Commun, 2020-02-07;11(1):766.
    Species: Ferret
    Sample Types: Whole Tissue
    Applications: IHC
  38. Statins can suppress dendritic cell-mediated Th2 responses through the repression of OX40-ligand and CCL17 expression
    Authors: N Inagaki-Ka, T Ito, M Inaba, Y Azuma, A Tanaka, PTX Vien, K Kibata, A Satake, S Nomura
    Eur. J. Immunol., 2019-07-11;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Isotype Control
  39. MUC20 expression marks the receptive phase of the human endometrium
    Authors: A Stepanjuk, M Koel, M Pook, M Saare, K Jääger, M Peters, K Krjutškov, S Ingerpuu, A Salumets
    Reprod. Biomed. Online, 2019-05-11;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  40. Function and Immunogenicity of Gene-corrected iPSC-derived Hepatocyte-Like Cells in Restoring Low Density Lipoprotein Uptake in Homozygous Familial Hypercholesterolemia
    Authors: H Okada, C Nakanishi, S Yoshida, M Shimojima, J Yokawa, M Mori, H Tada, T Yoshimuta, K Hayashi, T Yamano, R Hanayama, M Yamagishi, MA Kawashiri
    Sci Rep, 2019-03-18;9(1):4695.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  41. TYRO3 as a molecular target for growth inhibition and apoptosis induction in bladder cancer
    Authors: Florent Dufour, Linda Silina, Hélène Neyret-Kahn, Aura Moreno-Vega, Clémentine Krucker, Narjesse Karboul et al.
    British Journal of Cancer
  42. Antibody opsonization enhances MAIT cell responsiveness to bacteria via a TNF dependent mechanism
    Authors: Z Bánki, L Krabbendam, D Klaver, T Leng, S Kruis, H Mehta, B Müllauer, D Orth-Hölle, H Stoiber, CB Willberg, P Klenerman
    Immunol. Cell Biol., 2019-02-25;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  43. Regional Activation of Myosin II in Cancer Cells Drives Tumor Progression via a Secretory Cross-Talk with the Immune Microenvironment
    Authors: Mirella Georgouli, Cecilia Herraiz, Eva Crosas-Molist, Bruce Fanshawe, Oscar Maiques, Anna Perdrix et al.
    Cell
  44. Fluoxetine induces direct inhibitory effects on mesenchymal stem cell?derived osteoprogenitor cells independent of serotonin concentration
    Authors: SM Koura, M Salama, M El-Hussiny, MEA Khalil, A Lotfy, SA Hassan, SA Gad Elhak, MA Sobh
    Mol Med Rep, 2019-02-01;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  45. WNT Activates the AAK1 Kinase to Promote Clathrin-Mediated Endocytosis of LRP6 and Establish a Negative Feedback Loop
    Authors: Megan J. Agajanian, Matthew P. Walker, Alison D. Axtman, Roberta R. Ruela-de-Sousa, D. Stephen Serafin, Alex D. Rabinowitz et al.
    Cell Reports
  46. A Kaposi's Sarcoma-Associated Herpesvirus Infection Mechanism Is Independent of Integrins alpha 3 beta 1, alpha V beta 3, and alpha V beta 5
    Authors: Allison Alwan TerBush, Florianne Hafkamp, Hee Jun Lee, Laurent Coscoy
    Journal of Virology
  47. Imaging of Claudin-4 in Pancreatic Ductal Adenocarcinoma Using a Radiolabelled Anti-Claudin-4 Monoclonal Antibody
    Authors: Julia Baguña Torres, James C. Knight, Michael J. Mosley, Veerle Kersemans, Sofia Koustoulidou, Danny Allen et al.
    Molecular Imaging and Biology
  48. Molecular Correlates ofIn VitroResponses to Dacomitinib and Afatinib in Bladder Cancer
    Authors: S Tamura, Y Wang, B Veeneman, D Hovelson, A Bankhead, LJ Broses, G Lorenzatti, M Liebert, JR Rubin, KC Day, M Hussain, N Neamati, S Tomlins, PL Palmbos, P Grivas, ML Day
    Bladder Cancer, 2018-01-20;4(1):77-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  49. A Large Polysaccharide Produced by Helicobacter hepaticus Induces an Anti-inflammatory Gene Signature in Macrophages
    Authors: Camille Danne, Grigory Ryzhakov, Maria Martínez-López, Nicholas Edward Ilott, Fanny Franchini, Fiona Cuskin et al.
    Cell Host & Microbe
  50. Pigeon paramyxovirus type 1 from a fatal human case induces pneumonia in experimentally infected cynomolgus macaques (Macaca fascicularis)
    Authors: T Kuiken, P Buijs, P van Run, G van Ameron, M Koopmans, B van den Ho
    Vet. Res., 2017-11-21;48(1):80.
    Species: Primate
    Sample Types: Whole Tissue
    Applications: IHC-P
  51. MiR-205/YAP1 in Activated Fibroblasts of Breast Tumor Promotes VEGF-independent Angiogenesis through STAT3 Signaling
    Authors: YE Du, G Tu, G Yang, G Li, D Yang, L Lang, L Xi, K Sun, Y Chen, K Shu, H Liao, M Liu, Y Hou
    Theranostics, 2017-09-15;7(16):3972-3988.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  52. Innate activation of human primary epithelial cells broadens the host response to Mycobacterium tuberculosis in the airways
    Authors: AK Reuschl, MR Edwards, R Parker, DW Connell, L Hoang, A Halliday, H Jarvis, N Siddiqui, C Wright, S Bremang, SM Newton, P Beverley, RJ Shattock, OM Kon, A Lalvani
    PLoS Pathog., 2017-09-01;13(9):e1006577.
    Applications: Neutralization Control
  53. Erythropoietin Receptor-Mediated Molecular Crosstalk Promotes T Cell Immunoregulation and Transplant Survival
    Authors: Carolina Purroy, Robert L. Fairchild, Toshiaki Tanaka, William M. Baldwin, Joaquin Manrique, Joren C. Madsen et al.
    Journal of the American Society of Nephrology
  54. Phenotypic Differences between Asian and African Lineage Zika Viruses in Human Neural Progenitor Cells
    Authors: F Anfasa, JY Siegers, M van der Kr, N Mumtaz, V Stalin Raj, FMS de Vrij, W Widagdo, G Gabriel, S Salinas, Y Simonin, C Reusken, SA Kushner, MPG Koopmans, B Haagmans, BEE Martina, D van Riel
    mSphere, 2017-07-26;2(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  55. The expression of Nectin-4 on the surface of ovarian cancer cells alters their ability to adhere, migrate, aggregate, and proliferate
    Authors: Kristin L.M. Boylan, Petra C. Buchanan, Rory D. Manion, Dip M. Shukla, Kelly Braumberger, Cody Bruggemeyer et al.
    Oncotarget
  56. Expression of lectin-like transcript-1 in human tissues
    Authors: A Llibre, L Garner, A Partridge, GJ Freeman, P Klenerman, CB Willberg
    F1000Res, 2016-12-29;5(0):2929.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry Control, IHC
  57. EphA4 may contribute to microvessel remodeling in the hippocampal CA1 and CA3 areas in a mouse model of temporal lobe epilepsy
    Mol Med Rep, 2016-12-09;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  58. A mouse model of hereditary hemorrhagic telangiectasia generated by transmammary-delivered immunoblocking of BMP9 and BMP10
    Sci Rep, 2016-11-22;5(0):37366.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  59. Periodontitis-associated pathogens P. gingivalis and A. actinomycetemcomitans activate human CD14(+) monocytes leading to enhanced Th17/IL-17 responses
    Authors: Wan-Chien Cheng
    Eur J Immunol, 2016-08-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  60. USP6 oncogene promotes Wnt signaling by deubiquitylating Frizzleds
    Proc Natl Acad Sci USA, 2016-05-09;113(21):E2945-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  61. NKP30-B7-H6 Interaction Aggravates Hepatocyte Damage through Up-Regulation of Interleukin-32 Expression in Hepatitis B Virus-Related Acute-On-Chronic Liver Failure.
    Authors: Zou Y, Bao J, Pan X, Lu Y, Liao S, Wang X, Wang G, Lin D
    PLoS ONE, 2015-08-04;10(8):e0134568.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  62. Tetherin can restrict cell-free and cell-cell transmission of HIV from primary macrophages to T cells.
    Authors: Giese, Sebastia, Marsh, Mark
    PLoS Pathog, 2014-07-03;10(7):e1004189.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  63. Spontaneous CNV in a novel mutant mouse is associated with early VEGF-A-driven angiogenesis and late-stage focal edema, neural cell loss, and dysfunction.
    Authors: Nagai, Norihiro, Lundh von Leithner, Pete, Izumi-Nagai, Kanako, Hosking, Brett, Chang, Bo, Hurd, Ron, Adamson, Peter, Adamis, Anthony, Foxton, Richard, Ng, Yin Shan, Shima, David T
    Invest Ophthalmol Vis Sci, 2014-05-20;55(6):3709-19.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Isotype Control
  64. A polysaccharide virulence factor of a human fungal pathogen induces neutrophil apoptosis via NK cells.
    Authors: Robinet P, Baychelier F, Fontaine T, Picard C, Debre P, Vieillard V, Latge J, Elbim C
    J Immunol, 2014-04-30;192(11):5332-42.
    Species: Human
    Sample Types: Whole Blood
    Applications: Isotype Control
  65. Circulating conventional and plasmacytoid dendritic cell subsets display distinct kinetics during in vivo repeated allergen skin challenges in atopic subjects
    Authors: Stelios Vittorakis, Konstantinos Samitas, Sofia Tousa, Eleftherios Zervas, Maria Aggelakopoulou, Maria Semitekolou et al.
    BioMed Research International
  66. Targeting DNGR-1 (CLEC9A) with antibody/MUC1 peptide conjugates as a vaccine for carcinomas.
    Authors: Picco G, Beatson R, Taylor-Papadimitriou J, Burchell J
    Eur J Immunol, 2014-04-17;44(7):1947-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  67. Type I interferons directly inhibit regulatory T cells to allow optimal antiviral T cell responses during acute LCMV infection.
    Authors: Srivastava, Shivani, Koch, Meghan A, Pepper, Marion, Campbell, Daniel J
    J Exp Med, 2014-04-07;211(5):961-74.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  68. Leukocyte attraction by CCL20 and its receptor CCR6 in humans and mice with pneumococcal meningitis.
    Authors: Klein M, Brouwer M, Angele B, Geldhoff M, Marquez G, Varona R, Hacker G, Schmetzer H, Hacker H, Hammerschmidt S, van der Ende A, Pfister H, van de Beek D, Koedel U
    PLoS ONE, 2014-04-03;9(4):e93057.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  69. Characterizing functional domains for TIM-mediated enveloped virus entry.
    Authors: Moller-Tank S, Albritton L, Rennert P, Maury W
    J Virol, 2014-04-02;88(12):6702-13.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  70. Rapid target gene validation in complex cancer mouse models using re-derived embryonic stem cells.
    Authors: Huijbers I, Bin Ali R, Pritchard C, Cozijnsen M, Kwon M, Proost N, Song J, de Vries H, Badhai J, Sutherland K, Krimpenfort P, Michalak E, Jonkers J, Berns A
    EMBO Mol Med, 2014-01-08;6(2):212-25.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  71. The Human Adenovirus 5 L4 Promoter Is Activated by Cellular Stress Response Protein p53
    Authors: Jordan Wright, Keith N. Leppard
    Journal of Virology
  72. A hexadecylamide derivative of hyaluronan (HYMOVIS®) has superior beneficial effects on human osteoarthritic chondrocytes and synoviocytes than unmodified hyaluronan
    Authors: Margaret M Smith, Amy K Russell, Antonella Schiavinato, Christopher B Little
    Journal of Inflammation
  73. Propagation of human prostate cancer stem-like cells occurs through EGFR-mediated ERK activation.
    Authors: Rybak A, Ingram A, Tang D
    PLoS ONE, 2013-04-19;8(4):e61716.
    Species: Human
    Sample Types: Whole Cells
    Applications: Isotype Control
  74. Hepatitis C virus pathogen associated molecular pattern (PAMP) triggers production of lambda-interferons by human plasmacytoid dendritic cells.
    Authors: Stone, Amy E L, Giugliano, Silvia, Schnell, Gretja, Cheng, Linling, Leahy, Katelyn, Golden-Mason, Lucy, Gale, Michael, Rosen, Hugo R
    PLoS Pathog, 2013-04-18;9(4):e1003316.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization Control
  75. Mesothelial cells give rise to hepatic stellate cells and myofibroblasts via mesothelial-mesenchymal transition in liver injury.
    Proc Natl Acad Sci U S A, 2013-01-23;110(6):2324-9.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  76. TNF receptors regulate vascular homeostasis in zebrafish through a caspase-8, caspase-2 and P53 apoptotic program that bypasses caspase-3
    Authors: Raquel Espín, Francisco J. Roca, Sergio Candel, María P. Sepulcre, Juan M. González-Rosa, Francisca Alcaraz-Pérez et al.
    Disease Models & Mechanisms
  77. Human pituitary tumor-transforming gene 1 overexpression reinforces oncogene-induced senescence through CXCR2/p21 signaling in breast cancer cells
    Authors: Jhen-Wei Ruan, Yi-Chu Liao, Ingrid Lua, Ming-Hsun Li, Chih-Yi Hsu, Ji-Hshiung Chen
    Breast Cancer Research
  78. IL-15 Expression on RA Synovial Fibroblasts Promotes B Cell Survival.
    Authors: Benito-Miguel M, Garcia-Carmona Y, Balsa A, Bautista-Caro MB, Arroyo-Villa I, Cobo-Ibanez T, Bonilla-Hernan MG, De Ayala CP, Sanchez-Mateos P, Martin-Mola E, Miranda-Carus ME
    PLoS ONE, 2012-07-09;7(7):e40620.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  79. Cytotoxicity of CD56(bright) NK cells towards autologous activated CD4+ T cells is mediated through NKG2D, LFA-1 and TRAIL and dampened via CD94/NKG2A.
    Authors: Nielsen N, Odum N, Urso B, Lanier LL, Spee P
    PLoS ONE, 2012-02-22;7(2):e31959.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  80. CXCL12-Induced Monocyte-Endothelial Interactions Promote Lymphocyte Transmigration Across an in Vitro Blood-Brain Barrier
    Authors: Shumei Man, Barbara Tucky, Anne Cotleur, Judith Drazba, Yukio Takeshita, Richard M. Ransohoff
    Science Translational Medicine
  81. Inhibition of transcription factor specificity protein 1 alters the gene expression profile of keratinocytes leading to upregulation of kallikrein-related peptidases and thymic stromal lymphopoietin.
    Authors: Bin L, Kim BE, Hall CF
    J. Invest. Dermatol., 2011-07-14;131(11):2213-22.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  82. Reciprocal expression of IL-25 and IL-17A is important for allergic airways hyperreactivity.
    Authors: Barlow JL, Flynn RJ, Ballantyne SJ, McKenzie AN
    Clin. Exp. Allergy, 2011-07-04;41(10):1447-55.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Control
  83. Hepatic stellate cells express functional CXCR4: role in stromal cell-derived factor-1alpha-mediated stellate cell activation
    Authors: Feng Hong, Ana Tuyama, Ting Fang Lee, Johnny Loke, Ritu Agarwal, Xin Cheng et al.
    Hepatology
  84. Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulation.
    Authors: Reikvam H, Hatfield KJ, Oyan AM, Kalland KH, Kittang AO, Bruserud O
    Eur. J. Haematol., 2009-11-17;84(3):239-51.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  85. GAS6/Mer axis regulates the homing and survival of the E2A/PBX1-positive B-cell precursor acute lymphoblastic leukemia in the bone marrow niche.
    Authors: Shiozawa Y, Pedersen EA, Taichman RS
    Exp. Hematol., 2009-11-14;38(2):132-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  86. Autoimmunity in dry eye is due to resistance of Th17 to Treg suppression.
    Authors: Chauhan SK, El Annan J, Ecoiffier T, Goyal S, Zhang Q, Saban DR, Dana R
    J. Immunol., 2009-02-01;182(3):1247-52.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Control
  87. TLR7 Agonist 852A Inhibition of Tumor Cell Proliferation Is Dependent on Plasmacytoid Dendritic Cells and Type I IFN.
    Authors: Inglefield JR, Dumitru CD, Alkan SS, Gibson SJ, Lipson KE, Tomai MA, Larson CJ, Vasilakos JP
    J. Interferon Cytokine Res., 2008-04-01;28(4):253-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  88. Human epithelial cells establish direct antifungal defense through TLR4-mediated signaling.
    Authors: Weindl G, Naglik JR, Kaesler S, Biedermann T, Hube B, Korting HC, Schaller M
    J. Clin. Invest., 2007-12-01;117(12):3664-3672.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  89. Tumor-associated leukemia inhibitory factor and IL-6 skew monocyte differentiation into tumor-associated macrophage-like cells.
    Authors: Duluc D, Delneste Y, Tan F, Moles MP, Grimaud L, Lenoir J, Preisser L, Anegon I, Catala L, Ifrah N, Descamps P, Gamelin E, Gascan H, Hebbar M, Jeannin P
    Blood, 2007-09-11;110(13):4319-30.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Control
  90. Peroxisome proliferator-activated receptor-gamma agonists inhibit respiratory syncytial virus-induced expression of intercellular adhesion molecule-1 in human lung epithelial cells.
    Authors: Arnold R, Neumann M, Konig W
    Immunology, 2007-05-01;121(1):71-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  91. Hyperoxia causes angiopoietin 2-mediated acute lung injury and necrotic cell death.
    Authors: Bhandari V, Choo-Wing R, Lee CG, Zhu Z, Nedrelow JH, Chupp GL, Zhang X, Matthay MA, Ware LB, Homer RJ, Lee PJ, Geick A, de Fougerolles AR, Elias JA
    Nat. Med., 2006-11-05;12(11):1286-93.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Control
  92. Increased levels of neutrophil-activating peptide-2 in acute coronary syndromes: possible role of platelet-mediated vascular inflammation.
    Authors: Smith C, Damas JK, Otterdal K, ØIe E, Sandberg WJ, Yndestad A, Waehre T, Scholz H, Endresen K, Olofsson PS, Halvorsen B, Gullestad L, Froland SS, Hansson GK, Aukrust P
    J. Am. Coll. Cardiol., 2006-09-27;48(8):1591-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  93. LPS/CD14 activation triggers SGLT-1-mediated glucose uptake and cell rescue in intestinal epithelial cells via early apoptotic signals upstream of caspase-3.
    Authors: Yu LC, Turner JR, Buret AG
    Exp. Cell Res., 2006-06-22;312(17):3276-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  94. Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
    Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
    Clin. Immunol., 2006-06-16;120(2):220-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  95. Specific CXC but not CC chemokines cause elevated monocyte migration in COPD: a role for CXCR2.
    Authors: Traves SL, Smith SJ, Barnes PJ, Donnelly LE
    J. Leukoc. Biol., 2004-05-20;76(2):441-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  96. IL-11 is a crucial determinant of cardiovascular fibrosis
    Authors: S Schafer, S Viswanatha, AA Widjaja, WW Lim, A Moreno-Mor, DM DeLaughter, B Ng, G Patone, K Chow, E Khin, J Tan, SP Chothani, L Ye, OJL Rackham, NSJ Ko, NE Sahib, CJ Pua, NTG Zhen, C Xie, M Wang, H Maatz, S Lim, K Saar, S Blachut, E Petretto, S Schmidt, T Putoczki, N Guimarães-, H Wakimoto, S van Heesch, K Sigmundsso, SL Lim, JL Soon, VTT Chao, YL Chua, TE Tan, SM Evans, YJ Loh, MH Jamal, KK Ong, KC Chua, BH Ong, MJ Chakaramak, JG Seidman, CE Seidman, N Hubner, KYK Sin, SA Cook
    Nature, 2017-11-13;552(7683):110-115.
  97. Molecular Basis of Acute Cystitis Reveals Susceptibility Genes and Immunotherapeutic Targets.
    Authors: Ines Ambite, Manoj Puthia, Karoly Nagy, Caterina Cafaro, Aftab Nadeem, Daniel S C Butler, Gustav Rydström, Nina A Filenko, Björn Wullt, Thomas Miethke, Catharina Svanborg
    PloS Pathogens, 2016-10-12;0(0):1553-7374.

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Mouse IgG2A Isotype Control
By Anonymous on 04/07/2022
Application: B/N Sample Tested: LAD2 human mast cells Species: Human
Block/Neutralize Mouse IgG2A Isotype Control MAB003
Mouse IgG2A Isotype Control
By Abby Sukarto on 02/13/2020
Application: Flow Sample Tested: Bone Extracts Species: Human
Flow Cytometry Mouse IgG2A Isotype Control MAB003
Mouse IgG2A Isotype Control
By Anonymous on 01/08/2020
Application: Block/Neutralize CONTROL Sample Tested: Peripheral blood T cells Species: Human
Mouse IgG2A Isotype Control
By Abby Sukarto on 09/10/2019
Sample Tested: Bone Extracts Species: Human

Need optimization of your specific cell types and use the same amount as the primary antibody. Saw lots of binding for the isotype control.

Mouse IgG2A Isotype Control
By Anonymous on 08/04/2019
Application: WB Sample Tested: Recombinant protein Species: Mouse
Mouse IgG2A Isotype Control
By Anonymous on 03/23/2018
Application: Flow Sample Tested: Blood mononuclear cells (PBMCs) Species: Human
Mouse IgG2A Isotype Control
By Anonymous on 08/03/2016
Application: Flow Sample Tested: NCI-H146 Species: Human

NCI-H146 cells stained with test antibody (blue) or isotype control (red) at 10 ug/ml and then stained with secondary PE antibody.

Flow Cytometry Mouse IgG2A Isotype Control MAB003