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Mouse IgG2B Isotype Control

Catalog #: MAB0041
16 Citations Datasheet / COA / SDS
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MAB0041
Detection of Mouse IgG Isotype Control by Flow Cytometry
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Citations (16)
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Mouse IgG2B Isotype Control Summary

Specificity
Detects KLH. Does not cross-react with recombinant human (rh) 6Ckine, recombinant mouse (rm) 6Ckine, rhALP, rhBLC/BCA-1, rmBLC, rmC10, rhCCL28, rmCCL28, recombinant rat (rr) CINC-1, rrCINC-2 alpha, rrCINC-2 beta, rrCINC-3, rhCK beta 8-1, rmC-TACK, recombinant viral (rv) CMV UL146, rvCMV UL147, rmCRG-2, rhCXCL16, rmCXCL16, rhCXC-X3, rmCXC-X3, rhENA-78, rhEotaxin, rmEotaxin, rhEotaxin-2, rmEotaxin-2, rhEotaxin-3, rhFractalkine, rmFractalkine, rrFractalkine, rhGCP-2, rmGCP-2, rhGRO alpha, rhGRO beta, rhGRO gamma, rhHCC-1, rhHCC-4, rhI-309, rhIL-8, recombinant porcine IL-8, recombinat cotton rat (rcr) IP-10, rhIP-10, rhI-TAC, rmI-TAC, rmJE, rmKC, rhLeukotactin-1, rrLIX, rmLungkine, rhLymphotactin, rmLymphotactin, rmMARC, rhMCP-1, rhMCP-2, rmMCP-2, rhMCP-3, rhMCP-4, rmMCP-5, rvMCV type 2, rhMDC, rmMDC, rhMIG, rmMIG, rcrMIP-1 alpha, rhMIP-1 alpha, rmMIP-1 alpha, rcrMIP-1 beta, rhMIP-1 beta, rmMIP-1 beta, rhMIP-1δ, rmMIP-1 gamma, rmMIP-2, rhMIP-3 alpha, rmMIP-3 alpha, rrMIP-3 alpha, rhMIP-3 beta, rmMIP-3 beta, rvMIP-I, rvMIP-II, rvMIP-III, rhMPIF-1, rhNAP-2, rhPARC, rhPF4, rmPF4, rcrRANTES, rhRANTES, rmRANTES, rhSDF-1 alpha, rmSDF-1 alpha, rhSDF-1 beta, rhTARC, rmTARC, rmTCA-3, rhTeck, rmTeck, or rmThymus CK1.
Source
Monoclonal Mouse IgG2B Clone # 133303
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Control
Negative control for flow cytometry experiments. Use at the same concentration as the detection antibody.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Control Detection of Mouse IgG Isotype Control by Flow Cytometry View Larger

Detection of Mouse IgG Control by Flow Cytometry K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human MICA Monoclonal Antibody (Catalog # MAB1300, filled histogram) or Mouse IgG Isotype Control Antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgG2B

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G2B Fc
Alternate Names
IgG2B

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Citations for Mouse IgG2B Isotype Control

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. Distribution and Chemistry of Phoenixin-14, a Newly Discovered Sensory Transmission Molecule in Porcine Afferent Neurons
    Authors: Mazur, U;Lepiarczyk, E;Janikiewicz, P;?opie?ska-Biernat, E;Majewski, MK;Bossowska, A;
    International journal of molecular sciences
    Species: Porcine
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  2. KLRC1 knockout overcomes HLA-E-mediated inhibition and improves NK cell antitumor activity against solid tumors
    Authors: Alice Mac Donald, Delphine Guipouy, William Lemieux, Mario Harvey, Louis-Jean Bordeleau, David Guay et al.
    Frontiers in Immunology
  3. Antitumor effects of NK cells expanded by activation pre?processing of autologous feeder cells before irradiation in colorectal cancer
    Authors: Koh, EK;Lee, HR;Son, WC;Park, GY;Bae, J;Park, YS;
    Oncology letters
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. A JAM-A–tetraspanin– alpha v beta 5 integrin complex regulates contact inhibition of locomotion
    Authors: Daniel Kummer, Tim Steinbacher, Sonja Thölmann, Mariel Flavia Schwietzer, Christian Hartmann, Simone Horenkamp et al.
    Journal of Cell Biology
  5. Passive immunization against phosphorylated tau improves features of Huntington's disease pathology
    Authors: Melanie Alpaugh, Maria Masnata, Aurelie de Rus Jacquet, Eva Lepinay, Hélèna L. Denis, Martine Saint-Pierre et al.
    Molecular Therapy
  6. MUC20 expression marks the receptive phase of the human endometrium
    Authors: A Stepanjuk, M Koel, M Pook, M Saare, K Jääger, M Peters, K Krjutškov, S Ingerpuu, A Salumets
    Reprod. Biomed. Online, 2019-05-11;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  7. The expression of Nectin-4 on the surface of ovarian cancer cells alters their ability to adhere, migrate, aggregate, and proliferate
    Authors: Kristin L.M. Boylan, Petra C. Buchanan, Rory D. Manion, Dip M. Shukla, Kelly Braumberger, Cody Bruggemeyer et al.
    Oncotarget
  8. NKG2D-dependent activation of dendritic epidermal T cells in contact hypersensitivity.
    Authors: Nielsen M, Dyring-Andersen B, Schmidt J, Witherden D, Lovato P, Woetmann A, Odum N, Poulsen S, Havran W, Geisler C, Bonefeld C
    J Invest Dermatol, 2015-01-29;135(5):1311-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Isotype Control
  9. Selective susceptibility of human skin antigen presenting cells to productive dengue virus infection.
    Authors: Cerny D, Haniffa M, Shin A, Bigliardi P, Tan B, Lee B, Poidinger M, Tan E, Ginhoux F, Fink K
    PLoS Pathog, 2014-12-04;10(12):e1004548.
    Species: Human
    Sample Types: Whole Cells
    Applications: Isotype Control
  10. Absence of CC chemokine receptors 2a and 2b from human adipose lineage cells.
    Authors: Koenig C, Fischer-Posovszky P, Rojewski M, Tews D, Schrezenmeier H, Wabitsch M, Gierschik P, Moepps B
    Mol Cell Endocrinol, 2013-01-29;369(1):72-85.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Mycobacterium bovis bacilli Calmette-Guerin regulates leukocyte recruitment by modulating alveolar inflammatory responses.
    Authors: Andersson M, Lutay N, Hallgren O, Westergren-Thorsson G, Svensson M, Godaly G
    Innate Immun, 2011-11-04;18(3):531-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  12. Stemness markers characterize IGR-CaP1, a new cell line derived from primary epithelial prostate cancer.
    Authors: Chauchereau A, Al Nakouzi N, Gaudin C, Le Moulec S, Compagno D, Auger N, Benard J, Opolon P, Rozet F, Validire P, Fromont G, Fizazi K
    Exp. Cell Res., 2010-10-23;317(3):262-75.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  13. Induction of MHC class I-related chain B (MICB) by 5-aza-2'-deoxycytidine.
    Authors: Tang KF, He CX, Zeng GL, Wu J, Song GB, Shi YS, Zhang WG, Huang AL, Steinle A, Ren H
    Biochem. Biophys. Res. Commun., 2008-04-04;370(4):578-83.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  14. Human epithelial cells establish direct antifungal defense through TLR4-mediated signaling.
    Authors: Weindl G, Naglik JR, Kaesler S, Biedermann T, Hube B, Korting HC, Schaller M
    J. Clin. Invest., 2007-12-01;117(12):3664-3672.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  15. Endothelial damage from cytomegalovirus-specific host immune response can be prevented by targeted disruption of fractalkine-CX3CR1 interaction.
    Authors: Bolovan-Fritts CA, Spector SA
    Blood, 2007-09-25;111(1):175-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  16. The combined mechanism of bone morphogenetic protein- and calcium phosphate-induced skeletal tissue formation by human periosteum derived cells.
    Authors: Bolander J, Ji W, Geris L et al.
    Eur Cell Mater.

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