SARS-CoV-2 NSP9 Antibody

Catalog #: MAB10995 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10995-100
MAB10995-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

SARS-CoV-2 NSP9 Antibody Summary

Species Reactivity

SARS-CoV-2

Specificity

Detects SARS-CoV2 NSP9 in ELISA.

Source

Monoclonal Mouse IgG_2B Clone # 1044209

Purification

Protein A or G purified from cell culture supernatant

Immunogen

E. coli-derived SARS-CoV-2 NSP9 protein
Asn1-Gln113
Accession # YP_009725305.1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-SARS-CoV-2 NSP9 Monoclonal Antibody (Catalog # MAB10996).This product is intended for assay development on various assay platforms requiring antibody pairs.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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SARS-CoV-2 NSP9 ELISA Standard Curve. Recombinant SARS-CoV-2 NSP9 protein was serially diluted 2-fold and captured by Mouse Anti-SARS-CoV-2 NSP9 Monoclonal Antibody (Catalog # MAB10996) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-SARS-CoV-2 NSP9 Monoclonal Antibody (Catalog # MAB10995) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NSP9

Non-structural protein 9 (NSP9) is one of several functional proteins released by ORF1a-encoded protease cleavage of the pp1a and pp1ab replicase polyproteins expressed from the coronavirus (CoV) genome (1). The NSPs are involved in the replication and transcription of the viral RNA and not incorporated within the virion particles. Coronaviruses include various highly pathogenic strains such as SARS-CoV, MERS-CoV and SARS-CoV2 that have had significant impact on humans as well as strains that have negatively impacted livestock. NSP9 is a small 113 amino acid protein that forms a biologically active homodimer where each monomer consists of a beta barrel and C-terminal helical domain motif that promotes obligate dimerization (2,3). NSP9 is capable of binding nucleic acids in a nonsequence-specific manner with a preference of a single stranded RNA (4,5) although disruption of the dimeric interface appears to impact RNA binding (6). The NSP9 sequence is conserved across coronaviruses (3). NSP9 was shown to interact with other viral NSP proteins including NSP7, NSP8, and NSP12 (5,7,8). In addition, NSP9 has been shown to bind host cell proteins including DEAD-box RNA helicase 5 (DDX5), the ubiquitin E3 ubiquitin ligase MIB1, and elongation factor eIF4H in SARS-CoV2 and related viruses (9,10). The interactions of NSP9 with these host cell proteins promote viral replication (9,10) supporting the conclusion that NSP9 is important for virulence (2,3).

References