Viral Yellow Fever Virus NS1 Antibody

Catalog #: MAB97762 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB97762-100
MAB97762-SP

Viral Yellow Fever Virus NS1 ELISA Standard Curve.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Viral Yellow Fever Virus NS1 Antibody Summary

Species Reactivity

Viral

Specificity

Detects Yellow Fever Virus NS1 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 994718

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human embryonic kidney cell HEK293-derived Yellow Fever Virus NS1
Gln780-His1134
Accession # P03314

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Yellow Fever Virus NS1 Monoclonal Antibody (Catalog # MAB97761).This product is intended for assay development on various assay platforms requiring antibody pairs.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Viral Yellow Fever Virus NS1 ELISA Standard Curve. Recombinant Viral Yellow Fever Virus NS1 protein was serially diluted 2-fold and captured by Mouse Anti-Viral Yellow Fever Virus NS1 Monoclonal Antibody (Catalog # MAB97761) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Viral Yellow Fever Virus NS1 Monoclonal Antibody (Catalog # MAB97762) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Yellow Fever Virus NS1

Yellow fever virus (YFV) non-structural protein 1 (NS1) is produced by yellow fever virus (YFV), a mosquito-borne flavivirus that is responsible for numerous epidemics of yellow fever disease with high fatality rates (1). Flavivirus NS1 is a multifunctional virulence factor. The glycosylated NS1 exists as a membrane‑associated dimer after translocation into the endoplasmic reticulum lumen, where it is essential for viral genome replication (2). The secreted hexamer NS1 is involved in immune evasion and pathogenesis; it is identified as a potential diagnostic marker for early detection of the virus infections (2). Mature viral YFV NS1 contains 352 amino acids (afa) and shares high structural similarity to other flavivirus NS1 proteins, such as DENV and ZIKV.

References