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Human Erythropoietin IVD ELISA Kit - Quantikine

Catalog #: DEP00
74 Citations 6 Reviews Datasheet / COA / SDS
For In Vitro Diagnostic Use
Human Erythropoietin ELISA/Epo ELISA Benchtop Protocol
4 Images
Product Details
Procedure
Citations (74)
FAQs
Supplemental Products
Reviews (6)
Summary Sample Values Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human Erythropoietin Quantikine IVD ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours (Benchtop Protocol) or 2.5 hours (Shaker Protocol)
Sample Type & Volume Required Per Well
Serum (100 uL), EDTA Plasma (100 uL)
Sensitivity
0.6 mIU/mL
Assay Range
0.0 - 0 Not Assigned2.5 - 200 mIU/mL (Serum, EDTA Plasma),
Specificity
Natural and recombinant human Epo
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with other substances.

Sample Values

Erythropoietin concentrations were obtained from 123 normal individuals from the Minneapolis/St. Paul, Minnesota area. Using the nonparametric method for the analysis of reference values outlined in the NCCLS publication "How to Define, Determine, and Utilize Reference Intervals in the Clinical Laboratory" (NCCLS Document C28-P; Vol. 12, No. 2) the following reference ranges (2.5-97.5 percentile) were established for Epo in serum and EDTA plasma. Each testing laboratory should establish its own normal range.

Epo Normal Ranges 

SerumEDTA plasma
3.3-16.6 mlU/mL3.1-14.9 mlU/mL

Patients suffering from polycythemia rubra vera may have Epo concentrations within the normal range, whereas those suffering from secondary polycythemia may have elevated concentrations of serum Epo (17). Polycythemia rubra vera patients who undergo phlebotomy may have elevated serum Epo concentrations.

Patients suffering from most anemias will present with higher than normal concentrations of serum Epo, whereas those suffering from anemia associated with chronic renal failure may have serum Epo concentrations within the normal range of this assay (18). Anemic patients who receive transfusions may exhibit lower than expected serum Epo concentrations.

Abnormally high concentrations of serum Epo may also be observed in various other pathological states including renal neoplasms, benign tumors, polycystic kidney disease, renal cysts and hydronephrosis (19).

The results of this assay should be used in conjunction with information available from clinical evaluations and other diagnostic procedures.

Product Summary

The Quantikine® IVD Human Epo ELISA is designed to measure Epo levels in serum or plasma in less than 4.5 hours (or less than 2.5 hours using the shaker protocol) for the quantitative determination of erythropoietin (Epo) concentration in human serum and plasma as an aid in the diagnosis of anemia and polycythemia. For in vitro diagnostic use.

Precision

Benchtop Protocol: Precision (Precision within an assay) Four control pools were tested on one plate to assess intra-assay precision. Thirty assays were run using each protocol over a thirty day period with three separately manufactured lots of reagents
Shaker Protocol: Precision (Precision between assays) Four control pools were tested in separate assays to assess inter-assay precision. Thirty assays were run using each protocol over a thirty day period with three separately manufactured lots of reagents

Serum, EDTA Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 4 1 2 3 4
n 30 30 30 30 30 30 30 30
Mean (mIU/mL) 5.82 16.01 25.18 79.02 6.45 16.22 26.35 84.14
Standard Deviation 0 0 0 0 0 0 0 0
CV% 4.95 2.84 5.22 3.06 7.82 3.02 2.18 2.42

Recovery

The recovery of human Epo spiked to levels throughout the range of the assay was evaluated in 10 serum and plasma samples.                                                                                                                                                                                          

           

Specimen Type       

           

Protocol       

           

Amount Needed       

           

Mean Recovery       

SerumBenchtop52.2 mlU/mL100%
Shaker57.5 mlU/mL93%
Plasma (EDTA)Benchtop51.8 mlU/mL102%
Shaker55.9 mlU/mL105%

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of Epo were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Erythropoietin ELISA/Epo ELISA Linearity
Human Erythropoietin ELISA/Epo ELISA Linearity

Scientific Data

N/A Human Erythropoietin ELISA/Epo ELISA Benchtop Protocol View Larger

Human Erythropoietin ELISA/Epo ELISA Benchtop Protocol

N/A Human Erythropoietin ELISA/Epo ELISA Shaker Protocol View Larger

Human Erythropoietin ELISA/Epo ELISA Shaker Protocol

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Erythropoietin/EPO

Erythropoietin (Epo), a glycoprotein (~30,400 Daltons) produced primarily by the kidney, is the principal factor regulating red blood cell production (erythropoiesis) in mammals. Renal production of Epo is regulated by changes in oxygen availability. Under conditions of hypoxia, the level of Epo in the circulation increases and this leads to increased production of red blood cells. 

The over-expression of Epo may be associated with certain pathophysiological conditions (1, 2). Polycythemia exists when there is an overproduction of red blood cells (RBCs). Primary polycythemias, such as polycythemia vera, are caused by Epo-independent growth of erythrocytic progenitors from abnormal stem cells and low to normal levels of Epo are found in the serum of affected patients. On the other hand, various types of secondary polycythemias are associated with the production of higher than normal levels of Epo. The overproduction of Epo may be an adaptive response associated with conditions that produce tissue hypoxia, such as living at high altitude, chronic obstructive pulmonary disease, cyanotic heart disease, sleep apnea, high-affinity hemoglobinopathy, smoking, or localized renal hypoxia (1, 2). In other instances, excessive Epo levels are the result of production by neoplastic cells. Cases of increased Epo production and erythrocytosis have been reported for patients with renal carcinomas (3), benign renal tumors (4), Wilms' tumors, hepatomas (5), liver carcinomas (6), cerebellar hemangioblastomas (3, 7, 8), adrenal gland tumors (9), smooth muscle tumors (3, 9), and leiomyomas (10). 
Deficient Epo production is found in conjunction with certain forms of anemias. These include anemia of renal failure and end-stage renal disease (1, 2, 11), anemias of chronic disorders [chronic infections (1), autoimmune diseases (1), rheumatoid arthritis (12), AIDS (13), malignancies (14)], anemia of prematurity (2), anemia of hypothyroidism (2), and anemia of malnutrition (2). Many of these conditions are associated with the generation of IL-1 and TNF-alpha, factors that have been shown to be inhibitors of Epo activity (1, 15). Other forms of anemias, on the other hand, are due to Epo-independent causes and affected individuals show elevated levels of Epo (2). These forms include aplastic anemias, iron deficiency anemias, thalassemias, megaloblastic anemias, pure red cell aplasias, and myelodysplastic syndromes.

Entrez Gene IDs:
2056 (Human); 13856 (Mouse); 24335 (Rat)
Alternate Names:
ECYT5; EP; EPO; epoetin; Erythropoietin; MGC138142; MVCD2
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and specimens to room temperature before use. It is recommended that all specimens, standards, and controls be assayed in duplicate. Benchtop and shaker protocols are provided. The same protocol must be used throughout the assay.
  1.   Prepare all reagents as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 100 µL Standard, Control, or Specimen
  4.   Add 100 µL of Standard, control, or specimen to each well. Cover with a plate sealer.
  5.   For Benchtop Protocol: Incubate at room temperature for 2 hours.
    For Shaker Protocol: Incubate at room temperature for 1 hour on a horizontal orbital microplate shaker.
  6.   Thoroughly aspirate each well. Do not wash.
    7. 200 µL Conjugate
  7.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer.
  8.   For Benchtop Protocol: Incubate at room temperature for 2 hours.
    For Shaker Protocol: Incubate at room temperature for 1 hour on a horizontal orbital microplate shaker.
  9.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    10. 200 µL Substrate Solution
  10. Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20-25 minutes on the benchtop.
    11. 100 µL Stop Solution
  11. Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 600 nm.

Citations for Human Erythropoietin Quantikine IVD ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

74 Citations: Showing 1 - 10
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  1. RNA encoded peptide barcodes enable efficient in vivo screening of RNA delivery systems
    Authors: Odunze, U;Rustogi, N;Devine, P;Miller, L;Pereira, S;Vashist, S;Snijder, HJ;Corkill, D;Sabirsh, A;Douthwaite, J;Bond, N;Desai, A;
    Nucleic acids research
    Species: Human, Mouse
    Sample Types: Serum, Cell Culture Supernates
  2. Development of Lipid Nanoparticle Formulation for the Repeated Administration of mRNA Therapeutics
    Authors: Lee, Y;Jeong, M;Lee, G;Park, J;Jung, H;Im, S;Lee, H;
    Biomaterials research
    Species: Mouse
    Sample Types: Serum
  3. A Combined Biomarker That Includes Plasma Fibroblast Growth Factor 23, Erythropoietin, and Klotho Predicts Short- and Long-Term Morbimortality and Development of Chronic Kidney Disease in Critical Care Patients with Sepsis: A Prospective Cohort
    Authors: Toro, L;Rojas, V;Conejeros, C;Ayala, P;Parra-Lucares, A;Ahumada, F;Almeida, P;Silva, MF;Bravo, K;Pumarino, C;Tong, AM;Pinto, ME;Romero, C;Michea, L;
    Biomolecules
    Species: Human
    Sample Types: Plasma
  4. Abnormal whole-body energy metabolism in iron-deficient humans despite preserved skeletal muscle oxidative phosphorylation
    Authors: MC Frise, DA Holdsworth, AW Johnson, YJ Chung, MK Curtis, PJ Cox, K Clarke, DJ Tyler, DJ Roberts, PJ Ratcliffe, KL Dorrington, PA Robbins
    Scientific Reports, 2022-01-19;12(1):998.
    Species: Human
    Sample Types: Serum
  5. Erythrocytosis associated with IgA nephropathy
    Authors: C Cohen, S Coulon, K Bhukhai, A Neuraz, M Dussiot, G Fouquet, ML Stang, M Flamant, F Vrtovsnik, A Hummel, B Knebelmann, L Mesnard, E Rondeau, TT Maciel, F Favale, N Casadevall, T Nguyen-Kho, S Moutereau, C Legendre, M Benhamou, RC Monteiro, O Hermine, K El Karoui, IC Moura
    EBioMedicine, 2021-12-24;75(0):103785.
    Species: Human, Mouse, Transgenic Mouse
    Sample Types: Serum
  6. Heterogeneity of Hematological Response to Hypoxia and Short-Term or Medium-Term Bed Rest
    Authors: JT Royal, O Eiken, ME Keramidas, AC McDonnell, IB Mekjavic
    Frontiers in Physiology, 2021-12-14;12(0):777611.
    Species: Human
    Sample Types: Serum
  7. Modulation of Conductivity of Alginate Hydrogels Containing Reduced Graphene Oxide through the Addition of Proteins
    Authors: A Raslan, J Ciriza, AM Ochoa de R, ML Sanjuán, MS Toprak, P Galvez-Mar, L Saenz-Del-, JL Pedraz
    Pharmaceutics, 2021-09-15;13(9):.
    Species: Human
    Sample Types: Protein
  8. The Modifications of Haemoglobin, Erythropoietin Values and Running Performance While Training at Mountain vs. Hilltop vs. Seaside
    Authors: MC Man, C Ganera, GD B?rbule?, M Krzysztofi, AE Panaet, AI Cucui, DI Toh?nean, DI Alexe
    International Journal of Environmental Research and Public Health, 2021-09-08;18(18):.
    Species: Human
    Sample Types: Serum
  9. Ionizable lipid nanoparticles for in utero mRNA delivery
    Authors: RS Riley, MV Kashyap, MM Billingsle, B White, MG Alameh, SK Bose, PW Zoltick, H Li, R Zhang, AY Cheng, D Weissman, WH Peranteau, MJ Mitchell
    Science Advances, 2021-01-13;7(3):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  10. Acute and Chronic Effects of SGLT2 Inhibitor Empagliflozin on Renal Oxygenation and Blood Pressure Control in Nondiabetic Normotensive Subjects: A Randomized, Placebo-Controlled Trial
    Authors: A Zanchi, M Burnier, ME Muller, A Ghajarzade, M Maillard, N Loncle, B Milani, N Dufour, O Bonny, M Pruijm
    J Am Heart Assoc, 2020-06-20;0(0):e016173.
    Species: Human
    Sample Types: Plasma
  11. The Effect of Size, Maturation, Global Asphyxia, Cerebral Ischemia, and Therapeutic Hypothermia on the Pharmacokinetics of High-Dose Recombinant Erythropoietin in Fetal Sheep
    Authors: SK Dhillon, G Wassink, CA Lear, JO Davidson, NHG Holford, AJ Gunn, L Bennet
    Int J Mol Sci, 2020-04-25;21(9):.
    Species: Sheep
    Sample Types: Plasma
  12. Serum erythropoietin concentration and its correlation with stage of diabetic retinopathy
    Authors: S Davidovi?, N Babi?, S Jovanovi?, S Bariši?, D Grkovi?, A Miljkovi?
    BMC Ophthalmol, 2019-11-14;19(1):227.
    Species: Human
    Sample Types: Plasma, Serum
  13. Hypovolemia and reduced hemoglobin mass in patients with heart failure and preserved ejection fraction
    Authors: D Montero, T Haider, J Barthelmes, JP Goetze, S Cantatore, I Sudano, F Ruschitzka, AJ Flammer
    Physiol Rep, 2019-11-01;7(21):e14222.
    Species: Human
    Sample Types: Serum
  14. In-Depth Characterization of a Mifepristone-Regulated Expression System for AAV5-Mediated Gene Therapy in the Liver
    Authors: JM Liefhebber, R Martier, T Van der Zo, S Keskin, A Huseinovic, J Lubelski, B Blits, H Petry, P Konstantin
    Mol Ther Methods Clin Dev, 2019-05-16;13(0):512-525.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  15. Age-dependent impairment of the erythropoietin response to reduced central venous pressure in HFpEF patients
    Authors: D Montero, T Haider, J Barthelmes, JP Goetze, S Cantatore, C Lundby, I Sudano, F Ruschitzka, AJ Flammer
    Physiol Rep, 2019-03-01;7(5):e14021.
    Species: Human
    Sample Types: Plasma
  16. Serum erythropoietin levels, breast cancer and breast cancer-initiating cells
    Authors: K Bhat, K Sandler, S Duhachek-M, C Alli, F Cheng, NA Moatamed, CE Magyar, L Du, G Li, S McCloskey, E Vlashi, F Pajonk
    Breast Cancer Res., 2019-01-30;21(1):17.
    Species: Human
    Sample Types: Serum
  17. The opposing effects of acute inflammation and iron deficiency anemia on serum hepcidin and iron absorption in young women
    Authors: NU Stoffel, M Lazrak, S Bellitir, N El Mir, A El Hamdouc, A Barkat, C Zeder, D Moretti, H Aguenaou, MB Zimmermann
    Haematologica, 2019-01-10;0(0):.
    Species: Human
    Sample Types: Serum
  18. 3D Printed porous polyamide macrocapsule combined with alginate microcapsules for safer cell-based therapies
    Authors: L Saenz Del, J Ciriza, A Espona-Nog, X Illa, E Cabruja, G Orive, RM Hernández, R Villa, JL Pedraz, M Alvarez
    Sci Rep, 2018-05-31;8(1):8512.
  19. Generation of transgenic chickens expressing the human erythropoietin (hEPO) gene in an oviduct-specific manner: Production of transgenic chicken eggs containing human erythropoietin in egg whites
    Authors: MS Kwon, BC Koo, D Kim, YH Nam, XS Cui, NH Kim, T Kim
    PLoS ONE, 2018-05-30;13(5):e0194721.
    Species: Chicken
    Sample Types: Egg Whites
  20. Successful reprogramming of cellular protein production through mRNA delivered by functionalized lipid nanoparticles
    Authors: M Yanez Arte, T Kjellman, S Bartesaghi, S Wallin, X Wu, AJ Kvist, A Dabkowska, N Székely, A Radulescu, J Bergenholt, L Lindfors
    Proc. Natl. Acad. Sci. U.S.A., 2018-03-27;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  21. Increased serum iron in preeclamptic women is likely due to low hepcidin levels
    Authors: F Brunacci, VS Rocha, E De Carli, BP Espósito, R Ruano, C Colli
    Nutr Res, 2018-03-21;0(0):.
    Species: Human
    Sample Types: Serum
  22. Recombinant erythropoietin acutely decreases renal perfusion and decouples the renin-angiotensin-aldosterone system
    Authors: NJ Aachmann-A, SJ Christense, K Lisbjerg, P Oturai, PI Johansson, NH Holstein-R, NV Olsen
    Physiol Rep, 2018-03-01;6(5):.
    Species: Human
    Sample Types: Plasma
  23. Renal Handling of Ketones in Response to Sodium-Glucose Cotransporter 2 Inhibition in Patients With Type 2 Diabetes
    Authors: E Ferrannini, S Baldi, S Frascerra, B Astiarraga, E Barsotti, A Clerico, E Muscelli
    Diabetes Care, 2017-03-21;0(0):.
    Species: Human
    Sample Types: Serum
  24. Anaemia in hospitalised preschool children from a rural area in Mozambique: a case control study in search for aetiological agents
    Authors: C Moraleda, R Aguilar, L Quintó, T Nhampossa, M Renom, A Nhabomba, S Acácio, JJ Aponte, D Nhalungo, AH Achtman, L Schofield, H Martins, E Macete, PL Alonso, C Menéndez
    BMC Pediatr, 2017-02-28;17(1):63.
    Species: Human
    Sample Types:
  25. Effect of OSA on hypoxic and inflammatory markers during CPAP withdrawal: Further evidence from three randomized control trials
    Authors: Malcolm Kohler
    Respirology, 2016-11-18;0(0):.
    Species: Human
    Sample Types: Plasma
  26. Hemolytic anemia repressed hepcidin level without hepatocyte iron overload: lesson from G�nther disease model
    Authors: S Millot, C Delaby, B Moulouel, T Lefebvre, N Pilard, N Ducrot, C Ged, P Lett‚ron, L de Frances, JC Deybach, C Beaumont, L Gouya, H De Verneui, S Lyoumi, H Puy, Z Karim
    Haematologica, 2016-11-10;102(2):260-270.
    Species: Human
    Sample Types: Serum
  27. Improved Human Erythropoiesis and Platelet Formation in Humanized NSGW41 Mice
    Stem Cell Reports, 2016-09-08;0(0):.
    Species: Human
    Sample Types: Serum
  28. Diurnal changes of arterial oxygen saturation and erythropoietin concentration in male and female highlanders
    Physiol Rep, 2016-09-01;4(17):.
    Species: Human
    Sample Types: Plasma
  29. Therapeutic efficacy in a hemophilia B model using a biosynthetic mRNA liver depot system
    Gene Ther., 2016-06-30;23(10):699-707.
    Species: Human
    Sample Types: Serum
  30. Erythropoietin Slows Photoreceptor Cell Death in a Mouse Model of Autosomal Dominant Retinitis Pigmentosa
    Authors: Tonia S Rex
    PLoS ONE, 2016-06-14;11(6):e0157411.
    Species: Mouse
    Sample Types: Serum
  31. PlanHab: Hypoxia counteracts the erythropoietin suppression, but seems to exaggerate the plasma volume reduction induced by 3�weeks of bed rest
    Authors: ME Keramidas, IB Mekjavic, R Kölegård, A Choukèr, C Strewe, O Eiken
    Physiol Rep, 2016-04-13;4(7):.
    Species: Human
    Sample Types: Serum
  32. Revisiting Biomarkers of Total-Body and Partial-Body Exposure in a Baboon Model of Irradiation.
    Authors: Valente M, Denis J, Grenier N, Arvers P, Foucher B, Desangles F, Martigne P, Chaussard H, Drouet M, Abend M, Herodin F
    PLoS ONE, 2015-07-15;10(7):e0132194.
    Species: Primate - Papio anubis (Olive Baboon)
    Sample Types: Plasma
  33. Sequence-engineered mRNA Without Chemical Nucleoside Modifications Enables an Effective Protein Therapy in Large Animals.
    Authors: Thess A, Grund S, Mui B, Hope M, Baumhof P, Fotin-Mleczek M, Schlake T
    Mol Ther, 2015-06-08;23(9):1456-64.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Types: Serum
  34. Safety and angiogenic effects of systemic gene delivery of a modified erythropoietin.
    Authors: de Lucas Cerrillo A, Bond W, Rex T
    Gene Ther, 2015-02-26;22(5):365-73.
    Species: Hamster
    Sample Types: Cell Culture Supernates
  35. Mimicking hypoxia to treat anemia: HIF-stabilizer BAY 85-3934 (Molidustat) stimulates erythropoietin production without hypertensive effects.
    Authors: Flamme I, Oehme F, Ellinghaus P, Jeske M, Keldenich J, Thuss U
    PLoS ONE, 2014-11-13;9(11):e111838.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Types: Plasma
  36. Transgene regulation using the tetracycline-inducible TetR-KRAB system after AAV-mediated gene transfer in rodents and nonhuman primates.
    Authors: Le Guiner C, Stieger K, Toromanoff A, Guilbaud M, Mendes-Madeira A, Devaux M, Guigand L, Cherel Y, Moullier P, Rolling F, Adjali O
    PLoS ONE, 2014-09-23;9(9):e102538.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Types: Serum
  37. Erythropoietin rs1617640 G allele associates with an attenuated rise of serum erythropoietin and a marked decline of hemoglobin in hepatitis C patients undergoing antiviral therapy.
    Authors: Amanzada A, Goralczyk A, Reinhardt L, Moriconi F, Cameron S, Mihm S
    BMC Infect Dis, 2014-09-17;14(0):503.
    Species: Human
    Sample Types: Serum
  38. The relation of hepcidin to iron disorders, inflammation and hemoglobin in chronic kidney disease.
    Authors: Mercadel L, Metzger M, Haymann J, Thervet E, Boffa J, Flamant M, Vrtovsnik F, Houillier P, Froissart M, Stengel B
    PLoS ONE, 2014-06-30;9(6):e99781.
    Species: Human
    Sample Types: Serum
  39. Hematological parameters, and hematopoietic growth factors: EPO and IL-3 in response to whole-body cryostimulation (WBC) in military academy students.
    Authors: Szygula Z, Lubkowska A, Giemza C, Skrzek A, Bryczkowska I, Dolegowska B
    PLoS ONE, 2014-04-02;9(4):e93096.
    Species: Human
    Sample Types: Plasma
  40. Targeted delivery of erythropoietin by transcranial focused ultrasound for neuroprotection against ischemia/reperfusion-induced neuronal injury: a long-term and short-term study.
    Authors: Wu, Sheng-Ka, Yang, Ming-Tao, Kang, Kai-Hsia, Liou, Houng-Ch, Lu, Dai-Hua, Fu, Wen-Mei, Lin, Win-Li
    PLoS ONE, 2014-02-28;9(2):e90107.
    Species: Rat
    Sample Types: Tissue Homogenates
  41. Hypoxic response contributes to altered gene expression and precapillary pulmonary hypertension in patients with sickle cell disease.
    Authors: Zhang X, Zhang W, Ma S, Desai A, Saraf S, Miasniakova G, Sergueeva A, Ammosova T, Xu M, Nekhai S, Abbasi T, Casanova N, Steinberg M, Baldwin C, Sebastiani P, Prchal J, Kittles R, Garcia J, Machado R, Gordeuk V
    Circulation, 2014-02-10;129(16):1650-8.
    Species: Human
    Sample Types: Serum
  42. The effect of the neonatal Fc receptor on human IgG biodistribution in mice.
    Authors: Chen, Nancy, Wang, Weirong, Fauty, Scott, Fang, Yulin, Hamuro, Lora, Hussain, Azher, Prueksaritanont, Thomayan
    MAbs, 2014-01-09;6(2):502-8.
    Species: Mouse
    Sample Types: Plasma
  43. Mobilization of circulating endothelial progenitor cells correlates with the clinical course of hantavirus disease.
    Authors: Krautkramer E, Grouls S, Hettwer D, Rafat N, Tonshoff B, Zeier M
    J Virol, 2013-10-23;88(1):483-9.
    Species: Human
    Sample Types: Plasma
  44. Bone marrow mesenchymal stem cells in patients with beta thalassemia major: molecular analysis with attenuated total reflection-Fourier transform infrared spectroscopy study as a novel method.
    Stem Cells Dev., 2012-02-15;21(11):2000-11.
    Species: Human
    Sample Types: Bone Marrow Fluid
  45. Longevity of rAAV vector and plasmid DNA in blood after intramuscular injection in nonhuman primates: implications for gene doping.
    Authors: Ni W, Le Guiner C, Gernoux G
    Gene Ther., 2011-03-10;18(7):709-18.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Types: Serum
  46. Hypoxia-inducible factors in human pulmonary arterial hypertension: a link to the intrinsic myeloid abnormalities.
    Authors: Farha S, Asosingh K, Xu W, Sharp J, George D, Comhair S, Park M, Tang WH, Loyd JE, Theil K, Tubbs R, Hsi E, Lichtin A, Erzurum SC
    Blood, 2011-01-21;117(13):3485-93.
    Species: Human
    Sample Types: Plasma
  47. Increased plasma EPO and MIP-1 alpha are associated with recruitment of vascular progenitors but not CD34(+) cells in autologous peripheral blood stem cell grafts.
    Authors: Labonte L, Li Y, Yang L, Gillingham A, Halpenny M, Giulivi A, Sills T, Evans K, Zanke B, Allan DS
    Exp. Hematol., 2009-06-01;37(6):673-8.
    Species: Human
    Sample Types: Plasma
  48. Regulation of iron homeostasis in anemia of chronic disease and iron deficiency anemia: diagnostic and therapeutic implications.
    Authors: Theurl I, Aigner E, Theurl M, Nairz M, Seifert M, Schroll A, Sonnweber T, Eberwein L, Witcher DR, Murphy AT, Wroblewski VJ, Wurz E, Datz C, Weiss G
    Blood, 2009-03-17;113(21):5277-86.
    Species: Rat
    Sample Types: Serum
  49. Alterations of systemic and muscle iron metabolism in human subjects treated with low-dose recombinant erythropoietin.
    Authors: Robach P, Recalcati S, Girelli D, Gelfi C, Aachmann-Andersen NJ, Thomsen JJ, Norgaard AM, Alberghini A, Campostrini N, Castagna A, Vigano A, Santambrogio P, Kempf T, Wollert KC, Moutereau S, Lundby C, Cairo G
    Blood, 2009-03-04;113(26):6707-15.
    Species: Human
    Sample Types: Plasma
  50. Two large preoperative doses of erythropoietin do not reduce the systemic inflammatory response to cardiac surgery.
    Authors: Poulsen TD, Andersen LW, Steinbruchel D, Gotze JP, Jorgensen OS, Olsen NV
    J. Cardiothorac. Vasc. Anesth., 2008-10-22;23(3):316-23.
    Species: Human
    Sample Types: Plasma
  51. Iron metabolism in heterozygotes for hemoglobin E (HbE), alpha-thalassemia 1, or beta-thalassemia and in compound heterozygotes for HbE/beta-thalassemia.
    Authors: Zimmermann MB, Fucharoen S, Winichagoon P, Sirankapracha P, Zeder C, Gowachirapant S, Judprasong K, Tanno T, Miller JL, Hurrell RF
    Am. J. Clin. Nutr., 2008-10-01;88(4):1026-31.
    Species: Human
    Sample Types: Serum
  52. Pharmacodynamics and Pharmacokinetics of the Novel Thrombopoietin Mimetic Peptide RWJ-800088 in Humans.
    Authors: Liem-Moolenaar M, Cerneus D, Molloy C, End D, Brown K, de Kam M, Cohen A, van Hensbergen Y, Burggraaf J
    2008-10-01;84(0):481.
    Species: Human
    Sample Types: Plasma
  53. Pulmonary arterial hypertension and left-sided heart disease in sickle cell disease: clinical characteristics and association with soluble adhesion molecule expression.
    Authors: Klings ES, Anton Bland D, Rosenman D, Princeton S, Odhiambo A, Li G, Bernard SA, Steinberg MH, Farber HW
    Am. J. Hematol., 2008-07-01;83(7):547-53.
    Species: Human
    Sample Types: Plasma
  54. Influence of inflammation as measured by alpha-1-acid glycoprotein on iron status indicators among HIV-positive postpartum Zimbabwean women.
    Authors: Rawat R, Stoltzfus RJ, Ntozini R, Mutasa K, Iliff PJ, Humphrey JH
    Eur J Clin Nutr, 2008-05-28;63(6):787-93.
    Species: Human
    Sample Types: Serum
  55. Role of Gas6 in erythropoiesis and anemia in mice.
    Authors: Angelillo-Scherrer A, Burnier L, Lambrechts D, Fish RJ, Tjwa M, Plaisance S, Sugamele R, DeMol M, Martinez-Soria E, Maxwell PH, Lemke G, Goff SP, Matsushima GK, Earp HS, Chanson M, Collen D, Izui S, Schapira M, Conway EM, Carmeliet P
    J. Clin. Invest., 2008-02-01;118(2):583-96.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  56. Oral administration of doxycycline allows tight control of transgene expression: a key step towards gene therapy of retinal diseases.
    Authors: Stieger K, Mendes-Madeira A, Meur GL, Weber M, Deschamps JY, Nivard D, Provost N, Moullier P, Rolling F
    Gene Ther., 2007-10-04;14(23):1668-73.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Aqueous Humor
  57. Increased circulating endothelial progenitor cells in septic patients: correlation with survival.
    Authors: Rafat N, Hanusch C, Brinkkoetter PT, Schulte J, Brade J, Zijlstra JG, van der Woude FJ, van Ackern K, Yard BA, Beck GCh
    Crit. Care Med., 2007-07-01;35(7):1677-84.
    Species: Human
    Sample Types: Serum
  58. Selective functional exhaustion of hematopoietic progenitor cells in the bone marrow of patients with postinfarction heart failure.
    Authors: Kissel CK, Lehmann R, Assmus B, Aicher A, Honold J, Fischer-Rasokat U, Heeschen C, Spyridopoulos I, Dimmeler S, Zeiher AM
    J. Am. Coll. Cardiol., 2007-06-04;49(24):2341-9.
    Species: Human
    Sample Types: Serum
  59. Progress toward a nonviral gene therapy protocol for the treatment of anemia.
    Authors: Sebestyen MG, Hegge JO, Noble MA, Lewis DL, Herweijer H, Wolff JA
    Hum. Gene Ther., 2007-03-01;18(3):269-85.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Serum
  60. Strong iron demand during hypoxia-induced erythropoiesis is associated with down-regulation of iron-related proteins and myoglobin in human skeletal muscle.
    Authors: Robach P, Cairo G, Gelfi C, Bernuzzi F, Pilegaard H, Vigano A, Santambrogio P, Cerretelli P, Calbet JA, Moutereau S, Lundby C
    Blood, 2007-02-20;109(11):4724-31.
    Species: Human
    Sample Types: Serum
  61. Cerebrospinal fluid erythropoietin (EPO) in amyotrophic lateral sclerosis.
    Authors: Brettschneider J, Widl K, Schattauer D, Ludolph AC, Tumani H
    Neurosci. Lett., 2007-02-03;416(3):257-60.
    Species: Human
    Sample Types: Serum
  62. Evidence for downregulation of erythropoietin receptor in bone marrow erythroid cells of patients with chronic idiopathic neutropenia.
    Authors: Pontikoglou C, Liapakis G, Pyrovolaki K, Papadakis M, Bux J, Eliopoulos GD, Papadaki HA
    Exp. Hematol., 2006-10-01;34(10):1312-22.
    Species: Human
    Sample Types: Serum
  63. Erythropoiesis abnormalities contribute to early-onset anemia in patients with septic shock.
    Authors: Claessens YE, Fontenay M, Pene F, Chiche JD, Guesnu M, Hababou C, Casadevall N, Dhainaut JF, Mira JP, Cariou A
    Am. J. Respir. Crit. Care Med., 2006-03-30;174(1):51-7.
    Species: Human
    Sample Types: Plasma
  64. Circulating endothelial cells and angiogenic serum factors during neoadjuvant chemotherapy of primary breast cancer.
    Authors: Furstenberger G, von Moos R, Lucas R, Thurlimann B, Senn HJ, Hamacher J, Boneberg EM
    Br. J. Cancer, 2006-02-27;94(4):524-31.
    Species: Human
    Sample Types: Serum
  65. Long-term doxycycline-regulated transgene expression in the retina of nonhuman primates following subretinal injection of recombinant AAV vectors.
    Authors: Stieger K, Le Meur G, Lasne F, Weber M, Deschamps JY, Nivard D, Mendes-Madeira A, Provost N, Martin L, Moullier P, Rolling F
    Mol. Ther., 2006-01-25;13(5):967-75.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Vitreous Humor
  66. Effects of chronic hypoxia in vivo on the expression of human placental glucose transporters.
    Authors: Zamudio S, Baumann MU, Illsley NP
    Placenta, 2006-01-01;27(1):49-55.
    Species: Human
    Sample Types: Serum
  67. Haemoglobin E beta thalassaemia in Sri Lanka.
    Authors: Premawardhena A, Fisher CA, Olivieri NF, De Silva S, Arambepola M, Perera W, O&amp;apos;Donnell A, O'Donnell A, O'Donnell A, O'Donnell A, O'Donnell A, Peto TE, Viprakasit V, Merson L, Muraca G, Weatherall DJ
    Lancet, 2005-10-01;366(9495):1467-70.
    Species: Human
    Sample Types: Serum
  68. Long-term pharmacologically regulated expression of erythropoietin in primates following AAV-mediated gene transfer.
    Authors: Rivera VM, Gao GP, Grant RL, Schnell MA, Zoltick PW, Rozamus LW, Clackson T, Wilson JM
    Blood, 2004-10-26;105(4):1424-30.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Serum
  69. Expansion of human SCID-repopulating cells under hypoxic conditions.
    Authors: Danet GH, Pan Y, Luongo JL, Bonnet DA, Simon MC
    J. Clin. Invest., 2003-07-01;112(1):126-35.
    Species: Human
    Sample Types: Cell Culture Supernates
  70. Monoclonal myelopoiesis and subnormal erythropoietin concentration are independent risk factors for thromboembolic complications in essential thrombocythemia.
    Authors: Andreasson B, 9, Harrison C, Lindstedt G, Linch D, Kutti J
    Forward genetics in the mouse continues to be a useful and unbiased approach to identifying new genes and alleles with previously unappreciated roles in mammalian development and disease. Here, we rep, 2003-01-15;101(2):783.
    Species: Human
    Sample Types: Serum
  71. Lack of an immune response against the tetracycline-dependent transactivator correlates with long-term doxycycline-regulated transgene expression in nonhuman primates after intramuscular injection of recombinant adeno-associated virus.
    Authors: Favre D, Blouin V, Provost N, Spisek R, Porrot F, Bohl D, Marme F, Cherel Y, Salvetti A, Hurtrel B, Heard JM, Riviere Y, Moullier P
    J. Virol., 2002-11-01;76(22):11605-11.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Types: Serum
  72. Erythropoietin therapy for acute stroke is both safe and beneficial.
    Authors: Ehrenreich H, Hasselblatt M, Dembowski C, Cepek L, Lewczuk P, Stiefel M, Rustenbeck HH, Breiter N, Jacob S, Knerlich F, Bohn M, Poser W, Ruther E, Kochen M, Gefeller O, Gleiter C, Wessel TC, De Ryck M, Itri L, Prange H, Cerami A, Brines M, Siren AL
    Mol. Med., 2002-08-01;8(8):495-505.
    Species: Human
    Sample Types: Serum
  73. Evaluation of tumor necrosis factor-alpha and erythropoietin serum levels in B-cell chronic lymphocytic leukemia patients with anemia.
    Authors: Capalbo S, Battista C, Delia M, Ciancio A, De Santis G, Dargenio M, Diomede D, Liso V
    Acta Haematol., 2002-01-01;108(2):84-9.
    Species: Human
    Sample Types: Serum
  74. Adeno-associated virus-mediated delivery of erythropoietin leads to sustained elevation of hematocrit in nonhuman primates.
    Authors: Zhou S, Murphy JE, Escobedo JA, Dwarki VJ
    Gene Ther., 1998-05-01;5(5):665-70.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Types: Serum

FAQs

  1. What type of EPO is used in the standard for the Human Erythropoietin Quantikine® IVD® ELISA kit (Catalog # DEP00)?

    • The standard in this kit contains CHO (Chinese Hamster Ovary) cell-derived Recombinant Human EPO.

  2. Can the Human Erythropoietin Quantikine® IVD® ELISA kit (Catalog # DEP00) be used with heparin plasma?

    • Heparin plasma samples are not validated nor recommended for use in the Human Erythropoietin Quantikine® IVD® ELISA kit. During kit validation, results of in-house testing did not meet our specifications.

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Reviews for Human Erythropoietin Quantikine IVD ELISA Kit

Average Rating: 5 (Based on 6 Reviews)

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Human Erythropoietin Quantikine IVD ELISA Kit
By Anonymous on 06/07/2022
Sample Tested: Cell culture supernatant
Human Erythropoietin Quantikine IVD ELISA Kit DEP00
Human Erythropoietin Quantikine IVD ELISA Kit
By Anonymous on 08/22/2019
Sample Tested: EDTA Plasma
Human Erythropoietin Quantikine IVD ELISA Kit DEP00
Human Erythropoietin Quantikine IVD ELISA Kit
By Jeff Pimentel on 04/02/2018
Sample Tested: mouse serum
Human Erythropoietin Quantikine IVD ELISA Kit
By Leslie Priddy on 04/01/2018
Sample Tested: Serum
Human Erythropoietin Quantikine IVD ELISA Kit
By Jessica Lopez on 12/01/2017
Sample Tested: A375 human melanoma cell line
Human Erythropoietin Quantikine IVD ELISA Kit
By Anonymous on 01/23/2017
Sample Tested: EDTA Plasma

For a series of measurement of EPO concentrations in human blood samples, we internally validated this assay. Our results were: inter-assay CV for 6 samples with differernt concentrations throughout the assay range 5.0 - 9.5 % (5 runs on 5 days), intra-Assay precision was 4.1%. 2 different kit lots were used.
The Assay performs very well and reliable. Analysis of human blood samples was working well.

Human Erythropoietin Quantikine IVD ELISA Kit DEP00