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10X Ubiquitin Conjugation Reaction Buffer

Catalog #: B-70
7 Citations Datasheet / COA / SDS

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B-70 has been discontinued.
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Boston Biochem Supplemental Ubiquitin Reagents
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10X Ubiquitin Conjugation Reaction Buffer Summary

Specifications

Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-Species

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COA
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Background: Ubiquitin

Ubiquitin is a 76 amino acid (aa) protein that is ubiquitously expressed in all eukaryotic organisms. Ubiquitin is highly conserved with 96% aa sequence identity shared between human and yeast Ubiquitin, and 100% aa sequence identity shared between human and mouse Ubiquitin (1). In mammals, four Ubiquitin genes encode for two Ubiquitin-ribosomal fusion proteins and two poly-Ubiquitin proteins. Cleavage of the Ubiquitin precursors by deubiquitinating enzymes gives rise to identical Ubiquitin monomers each with a predicted molecular weight of 8.6 kDa. Conjugation of Ubiquitin to target proteins involves the formation of an isopeptide bond between the C-terminal glycine residue of Ubiquitin and a lysine residue in the target protein. This process of conjugation, referred to as ubiquitination or ubiquitylation, is a multi-step process that requires three enzymes: a Ubiquitin-activating (E1) enzyme, a Ubiquitin-conjugating (E2) enzyme, and a Ubiquitin ligase (E3). Ubiquitination is classically recognized as a mechanism to target proteins for degradation and as a result, Ubiquitin was originally named ATP-dependent Proteolysis Factor 1 (APF-1) (2,3). In addition to protein degradation, ubiquitination has been shown to mediate a variety of biological processes such as signal transduction, endocytosis, and post-endocytic sorting (4-7).

References
  1. Sharp, P.M. & W.-H. Li. (1987) Trends Ecol. Evol. 2:328.
  2. Ciechanover, A. et al. (1980 ) Proc. Natl. Acad. Sci. USA 77:1365.
  3. Hershko, A. et al. (1980) Proc. Natl. Acad. Sci. USA 77:1783.
  4. Greene, W. et al. (2012) PLoS Pathog. 8:e1002703.
  5. Tong, X. et al. (2012) J. Biol. Chem. 287:25280.
  6. Wei, W. et al. (2004) Nature 428:194.
  7. Wertz, I.E. et al. (2004) Nature 430:694.
Entrez Gene IDs
7314 (Human); 298693 (Rat)
Alternate Names
RPS27A; UBA52; UBB ubiquitin B; UBB; UBC; Ubiquitin

Citations for 10X Ubiquitin Conjugation Reaction Buffer

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. The E3 ubiquitin ligase MG53 inhibits hepatocellular carcinoma by targeting RAC1 signaling
    Authors: X Ma, X Ma, L Zhu, Y Zhao, M Chen, T Li, Y Lin, D Ma, C Sun, L Han
    Oncogenesis, 2022-07-20;11(1):40.  2022-07-20
  2. PARylation prevents the proteasomal degradation of topoisomerase I DNA-protein crosslinks and induces their deubiquitylation
    Authors: Y Sun, J Chen, SN Huang, YP Su, W Wang, K Agama, S Saha, LM Jenkins, JM Pascal, Y Pommier
    Nature Communications, 2021-08-18;12(1):5010.  2021-08-18
  3. A conserved SUMO pathway repairs topoisomerase DNA-protein cross-links by engaging ubiquitin-mediated proteasomal degradation
    Authors: Y Sun, LM Miller Jen, YP Su, KC Nitiss, JL Nitiss, Y Pommier
    Sci Adv, 2020-11-13;6(46):.  2020-11-13
  4. Autophagy induction in atrophic muscle cells requires ULK1 activation by TRIM32 through unanchored K63-linked polyubiquitin chains
    Authors: M Di Rienzo, M Antonioli, C Fusco, Y Liu, M Mari, I Orhon, G Refolo, F Germani, M Corazzari, A Romagnoli, F Ciccosanti, B Mandriani, MT Pellico, R De La Torr, H Ding, M Dentice, M Neri, A Ferlini, F Reggiori, M Kulesz-Mar, M Piacentini, G Merla, GM Fimia
    Sci Adv, 2019-05-08;5(5):eaau8857.  2019-05-08
  5. The ubiquitin-conjugating enzyme UBE2O modulates c-Maf stability and induces myeloma cell apoptosis
    Authors: Y Xu, Z Zhang, J Li, J Tong, B Cao, P Taylor, X Tang, D Wu, MF Moran, Y Zeng, X Mao
    J Hematol Oncol, 2017-07-03;10(1):132.  2017-07-03
  6. Metadherin/Astrocyte elevated gene-1 positively regulates the stability and function of forkhead box M1 during tumorigenesis
    Authors: Lixuan Yang
    Neuro-oncology, 2017-03-01;0(0):.  2017-03-01
  7. PINK1 Primes Parkin-Mediated Ubiquitination of PARIS in Dopaminergic Neuronal Survival
    Authors: Y Lee, DA Stevens, SU Kang, H Jiang, YI Lee, HS Ko, LA Scarffe, GE Umanah, H Kang, S Ham, TI Kam, K Allen, S Brahmachar, JW Kim, S Neifert, SP Yun, FC Fiesel, W Springer, VL Dawson, JH Shin, TM Dawson
    Cell Rep, 2017-01-24;18(4):918-932.  2017-01-24

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