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4-Hydroxynonenal Antibody

Catalog #: MAB3249
107 Citations 3 Reviews Datasheet / COA / SDS
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MAB3249
MAB3249-SP
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Detection of Human 4‑Hydroxynonenal by Western Blot.
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Citations (107)
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Reviews (3)
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4-Hydroxynonenal Antibody Summary

Specificity
Detects 4-Hydroxynonenal adducts of histidine residues. No cross-reactivity with nitrotyrosine, formaldehyde, glutaraldehyde, or 4‑hydroxy hexenaldehyde adducts is observed.
Source
Monoclonal Mouse IgG2B Clone # 198960
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH-coupled 4-Hydroxynonenal
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
5 µg/mL
See below
Immunohistochemistry
0.1-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human 4-Hydroxynonenal antibody by Western Blot. View Larger

Detection of Human 4‑Hydroxynonenal by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 4-Hydroxynonenal (4-HNE). PVDF membrane was probed with 1 µg/mL of Mouse Anti-4-Hydroxynonenal Monoclonal Antibody (Catalog # MAB3249) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for 4-Hydroxynonenal at adducts of histidine residues (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human 4-Hydroxynonenal antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human 4‑Hydroxynonenal by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 4-Hydroxynonenal (4-HNE), loaded at 0.2 mg/mL. Specific bands were detected for 4‑Hydroxynonenal at adducts of histidine residues kDa (as indicated) using 5 µg/mL of Mouse Anti-4‑Hydroxynonenal Monoclonal Antibody (Catalog # MAB3249). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunohistochemistry 4-Hydroxynonenal antibody in Human Prostate by Immunohistochemistry (IHC-P). View Larger

4‑Hydroxynonenal in Human Prostate. 4-Hydroxynonenal was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-4-Hydroxynonenal Monoclonal Antibody (Catalog # MAB3249) at 0.1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunohistochemistry 4-Hydroxynonenal antibody in Human Prostate Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

4‑Hydroxynonenal in Human Prostate Cancer Tissue. 4-Hydroxynonenal was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-4-Hydroxynonenal Monoclonal Antibody (Catalog # MAB3249) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunocytochemistry/ Immunofluorescence Detection of Mouse 4-Hydroxynonenal by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse 4-Hydroxynonenal by Immunocytochemistry/Immunofluorescence Diapocynin inhibits the formation of 3-nitrotyrosine (3-NT) and 4-hydroxynonenal (4-HNE) in the substantia nigra (SN) of MPTP-treated mice. (A) Representative Western blots illustrating the expression of 3-NT in SN. (B) Bar graph showing mean Western blot 3-NT/ beta -actin ratios ± SEM in SN of 6 mice per group. (C) Double labeling of tyrosine hydroxylase (TH) and 3-NT in SN region of ventral midbrain. (D) Representative Western blots illustrating the expression of 4-HNE in SN. (E) Bar graph showing mean Western blot 4-HNE/ beta -actin ratios ± SEM in SN of 6 mice per group. (F) Double labeling of TH and 4-HNE in SN region of ventral midbrain. Images were captured at 60× magnification. The SN zone is outlined in white dots. Inset pictures demonstrated colocalization of TH and 3-NT/4-HNE. ***P <0.001 vs the control group; **P <0.01 vs the control group; *P <0.05 vs the MPTP group; #P <0.001 vs the MPTP group. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23092448), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse 4-Hydroxynonenal by Western Blot View Larger

Detection of Mouse 4-Hydroxynonenal by Western Blot Diapocynin inhibits the formation of 3-nitrotyrosine (3-NT) and 4-hydroxynonenal (4-HNE) in the substantia nigra (SN) of MPTP-treated mice. (A) Representative Western blots illustrating the expression of 3-NT in SN. (B) Bar graph showing mean Western blot 3-NT/ beta -actin ratios ± SEM in SN of 6 mice per group. (C) Double labeling of tyrosine hydroxylase (TH) and 3-NT in SN region of ventral midbrain. (D) Representative Western blots illustrating the expression of 4-HNE in SN. (E) Bar graph showing mean Western blot 4-HNE/ beta -actin ratios ± SEM in SN of 6 mice per group. (F) Double labeling of TH and 4-HNE in SN region of ventral midbrain. Images were captured at 60× magnification. The SN zone is outlined in white dots. Inset pictures demonstrated colocalization of TH and 3-NT/4-HNE. ***P <0.001 vs the control group; **P <0.01 vs the control group; *P <0.05 vs the MPTP group; #P <0.001 vs the MPTP group. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23092448), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 4-Hydroxynonenal

4-hydroxy-2-nonenal (4-hydroxynonenal, 4-HNE) is a highly reactive aldehyde generated by the exposure of polyunsaturated fatty acids to peroxides and reactive oxygen species (ROS). It non-enzymatically forms stable protein adducts with histidine, lysine, and cysteine side chains that have been used as biomarkers for oxidative damage in cells. Conditions where 4-HNE immunoreactivity has been observed include include inflammation, neurodegenerative diseases, and ischemic damage to the heart and brain.

Alternate Names
(2E)-4-Hydroxy-2-nonenal ; 2-Nonenal, 4-hydroxy-, (2E,4R)-; 4 HNE; 4Hydroxynonenal; 4-Hydroxynonenal; 4-hydroxy-trans-2-nonenal; HNE

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Citations for 4-Hydroxynonenal Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

107 Citations: Showing 1 - 10
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  1. Oxidative inactivation of amyloid beta-degrading proteases by cholesterol-enhanced mitochondrial stress
    Authors: de Dios C, Bartolessis I, Roca-Agujetas V et al.
    Redox Biology
  2. FGF2 Alleviates Microvascular Ischemia-Reperfusion Injury by KLF2-mediated Ferroptosis Inhibition and Antioxidant Responses
    Authors: Fanfeng Chen, Jiayu Zhan, Mi Liu, Abdullah Al Mamun, Shanshan Huang, Yibing Tao et al.
    International Journal of Biological Sciences
  3. In Utero Exposure to 9-Tetrahydrocannabinol Leads to Postnatal Catch-up Growth and Dysmetabolism in the Adult Rat Liver
    Authors: Oke S, Lee K, Papp R Et Al.
    preprints
  4. Genetic deficiency or pharmacological inhibition of soluble epoxide hydrolase ameliorates high fat diet-induced pancreatic beta-cell dysfunction and loss
    Authors: Koike S, Hsu MF, Bettaieb A et al.
    Free radical biology & medicine
  5. Dietary Evodiamine Inhibits Atherosclerosis-Associated Changes in Vascular Smooth Muscle Cells
    Authors: Y Zha, Y Yang, Y Zhou, B Ye, H Li, J Liang
    International Journal of Molecular Sciences, 2023-04-03;24(7):.
  6. Red Light Mitigates the Deteriorating Placental Extracellular Matrix in Late Onset of Preeclampsia and Improves the Trophoblast Behavior
    Authors: Griffin J, Krolikowski J, Kounga K et al.
    Journal of Pregnancy
  7. Development of a cell‐free strategy to recover aged skeletal muscle after disuse
    Authors: Yu‐Fu Wu, Eduardo A. De La Toba, Svyatoslav Dvoretskiy, Rebecca Jung, Noah Kim, Laureen Daniels et al.
    The Journal of Physiology
  8. Suppressed hepatocyte proliferation via a ROS-HNE-P21 pathway is associated with nicotine- and cotinine-enhanced alcoholic fatty liver in mice
    Authors: Xue Chen, Kesheng Wang, Arthur I. Cederbaum, Yongke Lu
    Biochemical and Biophysical Research Communications
  9. Ferroptosis, a new target for treatment of renal injury and fibrosis in a 5/6 nephrectomy-induced CKD rat model
    Authors: Wang J, Wang Y, Liu Y et al.
    Cell death discovery
  10. Impact of genetic factors on antioxidant rescue of maternal diabetes-associated congenital heart disease
    Authors: Choudhury, TZ;Greskovich, SC;Girard, HB;Rao, AS;Budhathoki, Y;Cameron, EM;Conroy, S;Li, D;Zhao, MT;Garg, V;
    JCI insight
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  11. CD8+ T cell associated scoring model helps prognostic diagnosis and immunotherapy selection in patients with colon adenocarcinoma
    Authors: Zhao, Z;Chen, M;Sun, K;Gu, X;
    Heliyon
    Species: Human, Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  12. Astaxanthin improves lipotoxicity, lipid peroxidation and oxidative stress in kidney of sucrose-rich diet-fed rats
    Authors: Joubert, MBV;Ingaramo, PI;Collins, P;D'Alessandro, ME;
    The Journal of nutritional biochemistry
    Species: Rat
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  13. Aldehydes alter TGF-? signaling and induce obesity and cancer
    Authors: Yang, X;Bhowmick, K;Rao, S;Xiang, X;Ohshiro, K;Amdur, RL;Hassan, MI;Mohammad, T;Crandall, K;Cifani, P;Shetty, K;Lyons, SK;Merrill, JR;Vegesna, AK;John, S;Latham, PS;Crawford, JM;Mishra, B;Dasarathy, S;Wang, XW;Yu, H;Wang, Z;Huang, H;Krainer, AR;Mishra, L;
    Cell reports
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: Immunohistochemistry, Western Blot
  14. Cerium oxide nanoparticles (nanoceria) pretreatment attenuates cell death in the hippocampus and cognitive dysfunction due to repeated isoflurane anesthesia in newborn rats
    Authors: Kargi-Gemici, E;?engelen, A;Aksüt, Y;Akyol, O;?engiz-Erhan, S;Bay, M;Önay-Uçar, E;Selcan, A;Demirgan, S;
    Neurotoxicology
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  15. SLC7A11 protects luminal A breast cancer cells against ferroptosis induced by CDK4/6 inhibitors
    Authors: Cui, Y;Li, Y;Xu, Y;Liu, X;Kang, X;Zhu, J;Long, S;Han, Y;Xue, C;Sun, Z;Du, Y;Hu, J;Pan, L;Zhou, F;Xu, X;Li, X;
    Redox biology
    Species: Xenograft
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  16. Macrophage iron dyshomeostasis promotes aging-related renal fibrosis
    Authors: Wu, L;Lin, H;Li, S;Huang, Y;Sun, Y;Shu, S;Luo, T;Liang, T;Lai, W;Rao, J;Hu, Z;Peng, H;
    Aging cell
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  17. Olesoxime protects against cisplatin-induced acute kidney injury by attenuating mitochondrial dysfunction
    Authors: Wang, P;Ouyang, J;Zhou, K;Hu, D;Zhang, S;Zhang, A;Yang, Y;
    Biomedical journal
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  18. Short-term hypercaloric carbohydrate loading increases surgical stress resilience by inducing FGF21
    Authors: Agius, T;Emsley, R;Lyon, A;MacArthur, MR;Kiesworo, K;Faivre, A;Stavart, L;Lambelet, M;Legouis, D;de Seigneux, S;Golshayan, D;Lazeyras, F;Yeh, H;Markmann, JF;Uygun, K;Ocampo, A;Mitchell, SJ;Allagnat, F;Déglise, S;Longchamp, A;
    Nature communications
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  19. CU06-1004 alleviates oxidative stress and inflammation on folic acid-induced acute kidney injury in mice
    Authors: Bae, CR;Kim, Y;Kwon, YG;
    Journal of pharmacological sciences
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  20. CRISPR-Cas9 Engineered Extracellular Vesicles for the Treatment of Dominant Progressive Hearing Loss
    Authors: Pan, X;Huang, P;Ali, SS;Renslo, B;Hutchinson, TE;Erwin, N;Greenberg, Z;Ding, Z;Li, Y;Warnecke, A;Fernandez, NE;Staecker, H;He, M;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  21. Downregulation of TAZ elicits a mitochondrial redox imbalance and ferroptosis in lung epithelial cells exposed to diesel exhaust particles
    Authors: Lee, KY;Yang, CC;Shueng, PW;Wu, SM;Chen, CH;Chao, YC;Chang, YC;Han, CL;Chuang, HC;Lee, CC;Lin, CW;
    Ecotoxicology and environmental safety
    Species: Rats, Human
    Sample Types: Whole Tissue
    Applications: IHC
  22. Dental Pulp Stem Cell-Derived Conditioned Medium Alleviates Subarachnoid Hemorrhage-Induced Microcirculation Impairment by Promoting M2 Microglia Polarization and Reducing Astrocyte Swelling
    Authors: Ling-Yu Yang, Yong-Ren Chen, Jing-Er Lee, Kuo-Wei Chen, Hui-Tzung Luh, Yi-Tzu Chen et al.
    Translational Stroke Research
  23. Iron-overload-induced ferroptosis in mouse cerebral toxoplasmosis promotes brain injury and could be inhibited by Deferiprone
    Authors: Wang, C;Xie, L;Xing, Y;Liu, M;Yang, J;Gao, N;Cai, Y;
    PLoS neglected tropical diseases
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  24. Platelets Rich Plasma Increases Antioxidant Defenses of Tenocytes via Nrf2 Signal Pathway
    Authors: Tognoloni, A;Bartolini, D;Pepe, M;Di Meo, A;Porcellato, I;Guidoni, K;Galli, F;Chiaradia, E;
    International journal of molecular sciences
    Species: Equine
    Sample Types: Protein
    Applications: Western Blot
  25. Red cabbage juice-mediated gut microbiota modulation improves intestinal epithelial homeostasis and ameliorates colitis
    Authors: Jean Wilson, E;Sirpu Natesh, N;Ghadermazi, P;Pothuraju, R;Shanmugam, M;Prajapati, DR;Pandey, S;Kaifi, JT;Dodam, JR;Bryan, J;Lorson, CL;Watrelot, AA;Foster, JM;Mansel, TJ;Joshua Chan, SH;Batra, SK;Subbiah, J;Rachagani, S;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  26. Icariin Supplementation Suppresses the Markers of Ferroptosis and Attenuates the Progression of Nonalcoholic Steatohepatitis in Mice Fed a Methionine Choline-Deficient Diet
    Authors: Choi, J;Choi, H;Chung, J;
    International journal of molecular sciences
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  27. WBP2 restrains the lysosomal degradation of GPX4 to inhibit ferroptosis in cisplatin-induced acute kidney injury
    Authors: Deng, Z;Wang, Y;Liu, J;Zhang, H;Zhou, L;Zhao, H;Han, Y;Yan, S;Dong, Z;Wang, Y;Dai, Y;Deng, F;
    Redox biology
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  28. Repression of ferroptotic cell death by mitochondrial calcium signaling
    Authors: Wen H, Chen J, Zhao B et al.
    Research square
  29. Histone acetyltransferase P300 deficiency promotes ferroptosis of vascular smooth muscle cells by activating the HIF-1?/HMOX1 axis
    Authors: Shi J, Wang QH, Wei X et al.
    Molecular medicine (Cambridge, Mass.)
  30. Epigenetically silenced lncRNA SNAI3-AS1 promotes ferroptosis in glioma via perturbing the m6A-dependent recognition of Nrf2 mRNA mediated by SND1
    Authors: Jianglin Zheng, Qing Zhang, Zhen Zhao, Yue Qiu, Yujie Zhou, Zhipeng Wu et al.
    Journal of Experimental & Clinical Cancer Research
  31. Intraoperative Hemi-Diaphragm Electrical Stimulation Demonstrates Attenuated Mitochondrial Function without Change in Oxidative Stress in Cardiothoracic Surgery Patients
    Authors: Mankowski R, Wohlgemuth S, Bresciani G et al.
    Antioxidants
    Applications: Simple Western
  32. CYP1B1 inhibits ferroptosis and induces anti-PD-1 resistance by degrading ACSL4 in colorectal cancer
    Authors: C Chen, Y Yang, Y Guo, J He, Z Chen, S Qiu, Y Zhang, H Ding, J Pan, Y Pan
    Cell Death & Disease, 2023-04-14;14(4):271.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  33. Aldo-Keto Reductase 1C15 Characterization and Protection in Ischemic Brain Injury
    Authors: Yang T, Li Q, Fadoul G et al.
    Antioxidants (Basel, Switzerland)
  34. RanBP1: A Potential Therapeutic Target for Cancer Stem Cells in Lung Cancer and Glioma
    Authors: YJ Kahm, IG Kim, RK Kim
    International Journal of Molecular Sciences, 2023-04-06;24(7):.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  35. Cyanidin-3-glucoside inhibits ferroptosis in renal tubular cells after ischemia/reperfusion injury via the AMPK pathway
    Authors: Yi-Wei Du, Xiao-Kang Li, Ting-Ting Wang, Lu Zhou, Hui-Rong Li, Lan Feng et al.
    Molecular Medicine
  36. Asparagine protects pericentral hepatocytes during acute liver injury
    Authors: Yu Sun, Hadrien Demagny, Adrien Faure, Francesca Pontanari, Antoine Jalil, Nadia Bresciani et al.
    Journal of Clinical Investigation
  37. B cell-derived anti-beta 2 glycoprotein I antibody mediates hyperhomocysteinemia-aggravated hypertensive glomerular lesions by triggering ferroptosis
    Authors: Xing Du, Xiaolong Ma, Ying Tan, Fangyu Shao, Chun Li, Yang Zhao et al.
    Signal Transduction and Targeted Therapy
  38. ACSL1-induced ferroptosis and platinum resistance in ovarian cancer by increasing FSP1 N-myristylation and stability
    Authors: Qingyu Zhang, Ning Li, Limei Deng, Xingmei Jiang, Yuming Zhang, Leo Tsz On Lee et al.
    Cell Death Discovery
  39. Neutral amino acid transporter SLC38A2 protects renal medulla from hyperosmolarity-induced ferroptosis
    Authors: Du C, Xu H, Cao C et al.
    eLife
  40. The red blood cell as a mediator of endothelial dysfunction in patients with familial hypercholesterolemia and dyslipidemia
    Authors: Ali Mahdi, Tigist Wodaje, Oskar Kövamees, John Tengbom, Allan Zhao, Tong Jiao et al.
    Journal of Internal Medicine
  41. Investigating Therapeutic Effects of Indole Derivatives Targeting Inflammation and Oxidative Stress in Neurotoxin-Induced Cell and Mouse Models of Parkinson's Disease
    Authors: YJ Chiu, CH Lin, CY Lin, PN Yang, YS Lo, YC Chen, CM Chen, YR Wu, CF Yao, KH Chang, GJ Lee-Chen
    International Journal of Molecular Sciences, 2023-01-30;24(3):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  42. Investigating Therapeutic Effects of Indole Derivatives Targeting Inflammation and Oxidative Stress in Neurotoxin-Induced Cell and Mouse Models of Parkinson's Disease
    Authors: YJ Chiu, CH Lin, CY Lin, PN Yang, YS Lo, YC Chen, CM Chen, YR Wu, CF Yao, KH Chang, GJ Lee-Chen
    International Journal of Molecular Sciences, 2023;24(3):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  43. Cytokine Adsorber Use during DCD Heart Perfusion Counteracts Coronary Microvascular Dysfunction
    Authors: Lars Saemann, Fabio Hoorn, Adrian-Iustin Georgevici, Sabine Pohl, Sevil Korkmaz-Icöz, Gábor Veres et al.
    Antioxidants (Basel)
  44. Aberrant Transferrin and Ferritin Upregulation Elicits Iron Accumulation and Oxidative Inflammaging Causing Ferroptosis and Undermines Estradiol Biosynthesis in Aging Rat Ovaries by Upregulating NF-kappab-Activated Inducible Nitric Oxide Synthase: First Demonstration of an Intricate Mechanism
    Authors: SCW Sze, L Zhang, S Zhang, K Lin, TB Ng, ML Ng, KF Lee, JKW Lam, Z Zhang, KKL Yung
    International Journal of Molecular Sciences, 2022-10-21;23(20):.
    Species: Rat
    Sample Types: Tissue Lysates
    Applications: Western Blot
  45. Homozygous knockout of eEF2K alleviates cognitive deficits in APP/PS1 Alzheimer’s disease model mice independent of brain amyloid beta pathology
    Authors: Nicole P. Kasica, Xueyan Zhou, Hannah M. Jester, Caroline E. Holland, Alexey G. Ryazanov, Tom E. Forshaw et al.
    Frontiers in Aging Neuroscience
  46. Genistein attenuated oxidative stress, inflammation, and apoptosis in L-arginine induced acute pancreatitis in mice
    Authors: P Siriviriya, J Sriko, K Somanawat, M Chayanupat, N Klaikeaw, D Werawatgan
    BMC complementary medicine and therapies, 2022-08-04;22(1):208.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  47. Targeting Ferroptosis as a Novel Approach to Alleviate Aortic Dissection
    Authors: N Li, X Yi, Y He, B Huo, Y Chen, Z Zhang, Q Wang, Y Li, X Zhong, R Li, XH Zhu, Z Fang, X Wei, DS Jiang
    Oncogene, 2022-06-21;18(10):4118-4134.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  48. Impact and potential mechanism of effects of chronic moderate alcohol consumption on cardiac function in aldehyde dehydrogenase 2 gene heterozygous mice
    Authors: Qinfeng Hu, Hang Chen, Cheng Shen, Beijian Zhang, Xinyu Weng, Xiaolei Sun et al.
    Alcoholism: Clinical and Experimental Research
  49. Glia maturation factor-beta induces ferroptosis by impairing chaperone-mediated autophagic degradation of ACSL4 in early diabetic retinopathy
    Authors: C Liu, W Sun, T Zhu, S Shi, J Zhang, J Wang, F Gao, Q Ou, C Jin, J Li, JY Xu, J Zhang, H Tian, GT Xu, L Lu
    Redox Biology, 2022-03-18;52(0):102292.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC/IF
  50. Neuronal–glial communication perturbations in murine SOD1G93A spinal cord
    Authors: Michael MacLean, Raquel López-Díez, Carolina Vasquez, Paul F. Gugger, Ann Marie Schmidt
    Communications Biology
  51. Astaxanthin Relieves Testicular Ischemia-Reperfusion Injury-Immunohistochemical and Biochemical Analyses
    Authors: M Baškovi?, D Krsnik, M Himelreich, A Katuši? Bo, N Sin?i?, Z Sonicki, D Ježek
    Journal of Clinical Medicine, 2022-02-26;11(5):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  52. T lymphocyte-derived extracellular vesicles aggravate abdominal aortic aneurysm by promoting macrophage lipid peroxidation and migration via pyruvate kinase muscle isozyme 2
    Authors: G Dang, T Li, D Yang, G Yang, X Du, J Yang, Y Miao, L Han, X Ma, Y Song, B Liu, X Li, X Wang, J Feng
    Redox Biology, 2022-02-04;50(0):102257.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  53. Disinhibition-Like Behavior Correlates with Frontal Cortex Damage in an Animal Model of Chronic Alcohol Consumption and Thiamine Deficiency
    Authors: Marta Moya, Leticia López-Valencia, Borja García-Bueno, Laura Orio
    Biomedicines
  54. Enriched environment and visual stimuli protect the retinal pigment epithelium and photoreceptors in a mouse model of non-exudative age-related macular degeneration
    Authors: HH Dieguez, JS Calanni, HE Romeo, A Alaimo, MF González F, A Iaquinandi, MS Chianelli, MI Keller Sar, PH Sande, RE Rosenstein, D Dorfman
    Cell Death & Disease, 2021-12-04;12(12):1128.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  55. p113 isoform encoded by CUX1 circular RNA drives tumor progression via facilitating ZRF1/BRD4 transactivation
    Authors: Feng Yang, Anpei Hu, Yanhua Guo, Jianqun Wang, Dan Li, Xiaojing Wang et al.
    Molecular Cancer
  56. Overcoming the compensatory elevation of NRF2 renders hepatocellular carcinoma cells more vulnerable to disulfiram/copper-induced ferroptosis
    Authors: Xueying Ren, Yanchun Li, Yi Zhou, Wanye Hu, Chen Yang, Qiangan Jing et al.
    Redox Biology
  57. Trophoblasts Modulate the Ca2+ Oscillation and Contraction of Myometrial Smooth Muscle Cells by Small Extracellular Vesicle- (sEV-) Mediated Exporting of miR-25-3p during Premature Labor
    Authors: Lin Wang, Wenzhu Zhang, Ning Zou, Lijuan Zhang
    Oxidative Medicine and Cellular Longevity
  58. DHA exhibits synergistic therapeutic efficacy with cisplatin to induce ferroptosis in pancreatic ductal adenocarcinoma via modulation of iron metabolism
    Authors: Jing Du, Xu Wang, Yanchun Li, Xueying Ren, Yi Zhou, Wanye Hu et al.
    Cell Death & Disease
  59. Characterization of nonmotor behavioral impairments and their neurochemical mechanisms in the MitoPark mouse model of progressive neurodegeneration in Parkinson's disease
    Authors: Monica R. Langley, Shivani Ghaisas, Bharathi N. Palanisamy, Muhammet Ay, Huajun Jin, Vellareddy Anantharam et al.
    Experimental Neurology
  60. Ethyl Acetate Fraction of Amomum villosum var. xanthioides Attenuates Hepatic Endoplasmic Reticulum Stress-Induced Non-Alcoholic Steatohepatitis via Improvement of Antioxidant Capacities
    Authors: Jung-Hyo Cho, Jong-Suk Lee, Hyeong-Geug Kim, Hye Won Lee, Zhigang Fang, Hyeok-Hee Kwon et al.
    Antioxidants (Basel)
  61. Proteasome dysfunction under compromised redox metabolism dictates liver injury in NASH through ASK1/PPAR&gamma binodal complementary modules
    Authors: D Das, A Paul, A Lahiri, M Adak, SK Maity, A Sarkar, S Paul, P Chakrabart
    Redox Biology, 2021-06-11;45(0):102043.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  62. Discordant regulation of eIF2 kinase GCN2 and mTORC1 during nutrient stress
    Authors: J Misra, MJ Holmes, E T Mirek, M Langevin, HG Kim, KR Carlson, M Watford, XC Dong, TG Anthony, RC Wek
    Nucleic Acids Research, 2021-06-04;49(10):5726-5742.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  63. Sestrin proteins protect against lipotoxicity-induced oxidative stress in the liver via suppression of c-Jun N-terminal kinases: Short title: Sestrins ameliorate hepatic lipotoxicity
    Authors: Z Fang, HG Kim, M Huang, K Chowdhury, MO Li, S Liangpunsa, XC Dong
    Cellular and Molecular Gastroenterology and Hepatology, 2021-05-04;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  64. A role for aldehyde dehydrogenase (ALDH) 2 in angiotensin II-mediated decrease in angiogenesis of coronary endothelial cells
    Authors: Bipradas Roy, Suresh Selvaraj Palaniyandi
    Microvascular Research
  65. Acute liver injury following acetaminophen administration does not activate atrophic pathways in the mouse diaphragm
    Authors: CS Bruells, P Duschner, G Marx, G Gayan-Rami, N Frank, T Breuer, O Krenkel, F Tacke, JC Mossanen
    Scientific Reports, 2021-03-18;11(1):6302.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  66. Mitochondrial Iron Overload-Mediated Inhibition of Nrf2-HO-1/GPX4 Assisted ALI-Induced Nephrotoxicity
    Authors: Hui-Fang Deng, Lan-Xin Yue, Ning-Ning Wang, Yong-Qiang Zhou, Wei Zhou, Xian Liu et al.
    Frontiers in Pharmacology
  67. Fenpropathrin induces degeneration of dopaminergic neurons via disruption of the mitochondrial quality control system
    Authors: Zhigang Jiao, Yixuan Wu, Shaogang Qu
    Cell Death Discovery
  68. Sirt3 modulates fatty acid oxidation and attenuates cisplatin-induced AKI in mice
    Authors: M Li, CM Li, ZC Ye, J Huang, Y Li, W Lai, H Peng, TQ Lou
    J. Cell. Mol. Med., 2020-04-12;0(0):.
    Species: Mouse
    Sample Types: Tissue
    Applications: IHC-P
  69. Loss of CD36 protects against diet‐induced obesity but results in impaired muscle stem cell function, delayed muscle regeneration and hepatic steatosis
    Authors: Sandrine Verpoorten, Peggy Sfyri, David Scully, Robert Mitchell, Anastasia Tzimou, Vassilis Mougios et al.
    Acta Physiol (Oxf)
  70. Perinatal protein restriction with postnatal catch-up growth leads to elevated p66Shc and mitochondrial dysfunction in the adult rat liver
    Authors: Shelby L Oke, Gurjeev Sohi, Daniel B Hardy
    Reproduction
  71. Vasodilator oxyfedrine inhibits aldehyde metabolism and thereby sensitizes cancer cells to xCT‐targeted therapy
    Authors: Yuji Otsuki, Juntaro Yamasaki, Kentaro Suina, Shogo Okazaki, Naoyoshi Koike, Hideyuki Saya et al.
    Cancer Science
  72. Risperidone ameliorates prefrontal cortex neural atrophy and oxidative/nitrosative stress in brain and peripheral blood of rats with neonatal ventral hippocampus lesion
    Authors: H Tendilla-B, S Meneses-Pr, R Antonio Vá, M Tapia-Rodr, A Judith Váz, H Coatl-Cuay, D Martín-Her, KS MacDowell, L Garcés-Ram, JC Leza, G Flores
    J. Neurosci., 2019-09-13;0(0):.
    Species: Mouse
    Sample Types: Cell Lystates
    Applications: Western Blot
  73. Effects of curcumin on oxidative stress, inflammation and apoptosis in L-arginine induced acute pancreatitis in mice
    Authors: P Siriviriya, T Chingchit, N Klaikeaw, M Chayanupat, D Werawatgan
    Heliyon, 2019-08-27;5(8):e02222.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  74. Cell based therapy reduces secondary damage and increases extent of microglial activation following cortical injury
    Authors: Mary E. Orczykowski, Samantha M. Calderazzo, Eli Shobin, Monica A. Pessina, Adrian L. Oblak, Seth P. Finklestein et al.
    Brain Research
  75. Ferroptosis Induction in Pentylenetetrazole Kindling and Pilocarpine-Induced Epileptic Seizures in Mice
    Authors: Xiao-Yuan Mao, Hong-Hao Zhou, Wei-Lin Jin
    Frontiers in Neuroscience
  76. Mechanical ventilation causes diaphragm dysfunction in newborn lambs
    Authors: F Liang, G Emeriaud, DE Rassier, D Shang, E Gusev, SNA Hussain, M Sage, B Crulli, E Fortin-Pel, JP Praud, BJ Petrof
    Crit Care, 2019-04-16;23(1):123.
    Species: Ovine
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  77. Attenuation of oxidative stress-induced lesions in skeletal muscle in a mouse model of obesity-independent hyperlipidaemia and atherosclerosis through the inhibition of Nox2 activity
    Authors: Sfyri PP, Yuldasheva NY, Tzimou A et al.
    Free Radical Biology and Medicine
  78. Renal Medullary Interstitial COX-2 (Cyclooxygenase-2) Is Essential in Preventing Salt-Sensitive Hypertension and Maintaining Renal Inner Medulla/Papilla Structural Integrity
    Authors: Ming-Zhi Zhang, Suwan Wang, Yinqiu Wang, Yahua Zhang, Chuan Ming Ming Hao, Raymond C. Harris
    Hypertension
  79. Maternal Nicotine Exposure (MNE) leads to augmented expression of the anti-oxidant adipose tissue triglyceride lipase (ATGL) long-term in the white adipose of female rat offspring
    Authors: NG Barra, T VanDuzer, AC Holloway, DB Hardy
    Toxicol. Sci., 2018-07-01;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  80. Tocotrienol-rich fraction supplementation reduces hyperglycemia-induced skeletal muscle damage through regulation of insulin signaling and oxidative stress in type 2 diabetic mice
    Authors: H Lee, Y Lim
    J. Nutr. Biochem., 2018-03-21;57(0):77-85.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  81. The Addition of Manganese Porphyrins during Radiation Inhibits Prostate Cancer Growth and Simultaneously Protects Normal Prostate Tissue from Radiation Damage
    Authors: A Chatterjee, Y Zhu, Q Tong, EA Kosmacek, EZ Lichter, RE Oberley-De
    Antioxidants (Basel), 2018-01-25;7(1):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  82. Characterization of Dopaminergic System in the Striatum of Young Adult Park2-/- Knockout Rats
    Authors: JM Gemechu, A Sharma, D Yu, Y Xie, OM Merkel, A Moszczynsk
    Sci Rep, 2018-01-24;8(1):1517.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: Western Blot
  83. Age-dependent increase in angiopoietin-like protein 2 accelerates skeletal muscle loss in mice
    Authors: J Zhao, Z Tian, T Kadomatsu, P Xie, K Miyata, T Sugizaki, M Endo, S Zhu, H Fan, H Horiguchi, J Morinaga, K Terada, T Yoshizawa, K Yamagata, Y Oike
    J. Biol. Chem., 2017-11-30;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  84. Activation of ALDH1A1 in MDA-MB-468 breast cancer cells that over-express CYP2J2 protects against paclitaxel-dependent cell death mediated by reactive oxygen species
    Authors: Sarah E. Allison, Yongjuan Chen, Nenad Petrovic, Jian Zhang, Kirsi Bourget, Peter I. Mackenzie et al.
    Biochemical Pharmacology
  85. Ablation of ferroptosis regulator glutathione peroxidase 4 in forebrain neurons promotes cognitive impairment and neurodegeneration
    Authors: WS Hambright, RS Fonseca, L Chen, R Na, Q Ran
    Redox Biol, 2017-02-01;12(0):8-17.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  86. Gamma-tocopherol supplementation ameliorated hyper-inflammatory response during the early cutaneous wound healing in alloxan-induced diabetic mice
    Authors: J Shin, SJ Yang, Y Lim
    Exp. Biol. Med. (Maywood), 2016-12-13;242(5):505-515.
    Species: Mouse
    Sample Types: Plasma
    Applications: Western Blot
  87. Ketogenic diet decreases oxidative stress and improves mitochondrial respiratory complex activity
    Authors: Tiffany Greco, Thomas C Glenn, David A Hovda, Mayumi L Prins
    Journal of Cerebral Blood Flow & Metabolism
  88. Vitamin D deficiency impairs skeletal muscle function in a smoking mouse model
    Authors: Nele Cielen, Nele Heulens, Karen Maes, Geert Carmeliet, Chantal Mathieu, Wim Janssens et al.
    Journal of Endocrinology
  89. Oxidative Stress Triggers Body-Wide Skipping of Multiple Exons of the Spinal Muscular Atrophy Gene
    Authors: J Seo, NN Singh, EW Ottesen, S Sivanesan, M Shishimoro, RN Singh
    PLoS ONE, 2016-04-25;11(4):e0154390.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  90. Sulindac attenuates valproic acid-induced oxidative stress levels in primary cultured cortical neurons and ameliorates repetitive/stereotypic-like movement disorders in Wistar rats prenatally exposed to valproic acid
    Authors: YINGHUA ZHANG, CAILING YANG, GUOYAN YUAN, ZHONGPING WANG, WEIGANG CUI, RUIXI LI
    International Journal of Molecular Medicine
  91. Differential metabolism of 4-hydroxynonenal in liver, lung and brain of mice and rats
    Authors: Ruijin Zheng, Ana-Cristina Dragomir, Vladimir Mishin, Jason R. Richardson, Diane E. Heck, Debra L. Laskin et al.
    Toxicology and Applied Pharmacology
  92. Toll-like 4 receptor inhibitor TAK-242 decreases neuroinflammation in rat brain frontal cortex after stress.
    Authors: Garate I, Garcia-Bueno B, Madrigal J, Caso J, Alou L, Gomez-Lus M, Leza J
    J Neuroinflammation, 2014-01-11;11(0):8.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  93. Characterization of the adverse effects of nicotine on placental development: in vivo and in vitro studies.
    Authors: Holloway, A C, Salomon, A, Soares, M J, Garnier, V, Raha, S, Sergent, F, Nicholson, C J, Feige, J J, Benharouga, M, Alfaidy, N
    Am J Physiol Endocrinol Metab, 2013-12-24;306(4):E443-56.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  94. Oxidative damage and myofiber degeneration in the gastrocnemius of patients with peripheral arterial disease
    Authors: Dustin J Weiss, George P Casale, Panagiotis Koutakis, Aikaterini A Nella, Stanley A Swanson, Zhen Zhu et al.
    Journal of Translational Medicine
  95. The asymmetric segregation of damaged proteins is stem cell-type dependent.
    Authors: Bufalino, Mary Ros, DeVeale, Brian, van der Kooy, Derek
    J Cell Biol, 2013-05-06;201(4):523-30.
    Species: Drosophila
    Sample Types: Whole Tissue
    Applications: IHC
  96. Interactions between vascular wall and perivascular adipose tissue reveal novel roles for adiponectin in the regulation of endothelial nitric oxide synthase function in human vessels.
    Authors: Margaritis M, Antonopoulos A, Digby J, Lee R, Reilly S, Coutinho P, Shirodaria C, Sayeed R, Petrou M, De Silva R, Jalilzadeh S, Demosthenous M, Bakogiannis C, Tousoulis D, Stefanadis C, Choudhury R, Casadei B, Channon K, Antoniades C
    Circulation, 2013-04-26;127(22):2209-21.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  97. Anti-inflammatory and neuroprotective effects of an orally active apocynin derivative in pre-clinical models of Parkinson's disease.
    J Neuroinflammation, 2012-10-23;9(0):241.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  98. Transgenic control of mitochondrial fission induces mitochondrial uncoupling and relieves diabetic oxidative stress.
    Diabetes, 2012-06-14;61(8):2093-104.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  99. 4-Hydroxynonenal differentially regulates adiponectin gene expression and secretion via activating PPARgamma and accelerating ubiquitin-proteasome degradation.
    Authors: Wang Z, Dou X, Gu D, Shen C, Yao T, Nguyen V, Braunschweig C, Song Z
    Mol. Cell. Endocrinol., 2011-11-10;349(2):222-31.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  100. Methamphetamine oxidatively damages parkin and decreases the activity of 26S proteasome in vivo
    Authors: Anna Moszczynska, Bryan K. Yamamoto
    Journal of Neurochemistry
  101. Normoxic versus hyperoxic resuscitation in pediatric asphyxial cardiac arrest: effects on oxidative stress.
    Authors: Walson KH, Tang M, Glumac A, Alexander H, Manole MD, Ma L, Hsia CJ, Clark RS, Kochanek PM, Kagan VE, Bayr H
    Crit. Care Med., 2011-02-01;39(2):335-43.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  102. Mitochondrial dysfunction and increased reactive oxygen species impair insulin secretion in sphingomyelin synthase 1-null mice.
    Authors: Yano M, Watanabe K, Yamamoto T, Ikeda K, Senokuchi T, Lu M, Kadomatsu T, Tsukano H, Ikawa M, Okabe M, Yamaoka S, Okazaki T, Umehara H, Gotoh T, Song WJ, Node K, Taguchi R, Yamagata K, Oike Y
    J. Biol. Chem., 2010-11-29;286(5):3992-4002.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  103. Macrophage delivery of therapeutic nanozymes in a murine model of Parkinson’s disease
    Authors: Anna M. Brynskikh, Yuling Zhao, R. Lee Mosley, Shu Li, Michael D. Boska, Natalia L. Klyachko et al.
    Nanomedicine (Lond)
  104. Cell sex determines anoikis resistance in vascular smooth muscle cells.
    Authors: Straface E, Vona R, Gambardella L, Ascione B, Marino M, Bulzomi P, Canu S, Coinu R, Rosano G, Malorni W, Franconi F
    FEBS Lett., 2009-10-03;583(21):3448-54.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  105. Autoantibodies to the C-terminal subunit of RLIP76 induce oxidative stress and endothelial cell apoptosis in immune-mediated vascular diseases and atherosclerosis.
    Authors: Margutti P, Matarrese P, Conti F, Colasanti T, Delunardo F, Capozzi A, Garofalo T, Profumo E, Rigano R, Siracusano A, Alessandri C, Salvati B, Valesini G, Malorni W, Sorice M, Ortona E
    Blood, 2007-11-09;111(9):4559-70.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  106. Loss of cell–cell adhesion triggers cell migration through Rac1-dependent ROS generation
    Authors: Yu-Hsuan Chen, Jinn-Yuan Hsu, Ching-Tung Chu, Yao-Wen Chang, Jia-Rong Fan, Muh-Hwa Yang et al.
    Life Science Alliance
  107. Prevention of LPS-Induced Acute Kidney Injury in Mice by Bavachin and Its Potential Mechanisms
    Authors: Ban K, Nam G, Kim D et al.
    Antioxidants

FAQs

  1. For the Western Blot data image for Catalog # MAB3249, how long were the HepG2 cells treated with 4-HNE prior to analysis?

    • The HepG2 cells were treated with 100 µM 4-HNE for 1 hour before lysing the cells.

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By Mary-Kate Hayward on 03/22/2024
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Breast cancer tissue Species: Human

OCT embedded tissues

4-Hydroxynonenal Antibody
By Anonymous on 08/11/2021
Application: IHC Sample Tested: Liver tissue Species: Mouse
Immunohistochemistry 4-Hydroxynonenal Antibody MAB3249
4-Hydroxynonenal Antibody
By Anonymous on 03/29/2021
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Aortic valves Species: Mouse

-Fixed in 10% neutral buffered formalin followed by dehydration process
-10 um thick cryosection
- Blocking: Normal Goat Serum (NGS)
- Primary Ab (4-HNE): diluted in NGS at 20 ug/mL; 4C overnight
- Secondary (AF488): 1:100 dilution in NGS; room temp 1hr
- Vector anti-fade mounting media with DAPI

Immunocytochemistry/Immunofluorescence 4-Hydroxynonenal Antibody MAB3249