7-Aminoactinomycin D

Biological Activity

Used for: cell cycle studies, selective detection of dead cells by flow cytometry, chromosome banding analysis, cell sorting, and apoptosis quantification.

Application: fluorescence microscopy and flow cytometry.

Properties and Photophysical Data: G-C base-specific DNA intercalator that forms stable complexes with DNA. May be used in combination with FITC (Cat. No. 5440) or phycoerythrin (PE). Fluorescent analogue of actinomycin D (Cat. No. 1229). Can be excited using 488 nm (blue), 532 nm (green), and 561 nm (yellow-green) laser lines. Suitable for multicolor fluorescence microscopy experiments. Excitation and emission maxima (λ) are 549 nm and 655 nm, respectively.

Optical Data for 7-Aminoactinomycin D

λabs	549 nm
λem	655 nm
Application	Fluorescence Microscopy, Flow Cytometry

Technical Data

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

1. 7-Amino-actinomycin D as a specific fluorophore for DNA content analysis by laser flow cytometry.
   Zelenin et al.
   Cytometry, 1984;5:348
2. Dead cell discrimination with 7-amino-actinomycin D in combination with dual color immunofluorescence in single laser flow cytometry.
   Schmid et al.
   Cytometry, 1992;13:204
3. The use of 7-amino actinomycin D in identifying apoptosis: Simplicity of use and broad spectrum of application compared with other techniques.
   Philpott et al.
   Blood, 1996;87:2244

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