Home / Proteasome Substrates / Ac-Ala-Asn-Trp-AMC (Ac-ANW-AMC)

Ac-Ala-Asn-Trp-AMC (Ac-ANW-AMC)

Catalog #: S-320
3 Citations 2 Reviews Datasheet / COA / SDS

Discontinued Product

S-320 has been discontinued.
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Product Details
Citations (3)
FAQs
Reviews (2)
Summary Product Datasheets

Ac-Ala-Asn-Trp-AMC (Ac-ANW-AMC) Summary

Specifications

Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-Species

Product Datasheets

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Product Datasheet
COA
SDS

Citations for Ac-Ala-Asn-Trp-AMC (Ac-ANW-AMC)

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. H727 cells are inherently resistant to the proteasome inhibitor carfilzomib, yet require proteasome activity for cell survival and growth
    Authors: MJ Lee, Z Miller, JE Park, D Bhattarai, W Lee, KB Kim
    Sci Rep, 2019-03-11;9(1):4089.  2019-03-11
  2. Immunoproteasome functions explained by divergence in cleavage specificity and regulation
    Authors: MB Winter, F La Greca, S Arastu-Kap, F Caiazza, P Cimermanci, TJ Buchholz, JL Anderl, M Ravalin, MF Bohn, A Sali, AJ O'Donoghue, CS Craik
    Elife, 2017-11-28;6(0):.  2017-11-28
  3. Brief treatment with a highly selective immunoproteasome inhibitor promotes long-term cardiac allograft acceptance in mice
    Proc. Natl. Acad. Sci. U.S.A, 2016-12-12;113(52):E8425-E8432.  2016-12-12

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Reviews for Ac-Ala-Asn-Trp-AMC (Ac-ANW-AMC)

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Ac-Ala-Asn-Trp-AMC (Ac-ANW-AMC)
By TAKASHI SHIMADA on 05/02/2020

S-320 hydrolysis confirmation by LCMS-SRM
S-320: 1 uM
i20S: 15 nM
PA28a: 150 nM
Reaction: 37 °C for 5 h
1:S-320 Ac-ANW-AMC (589.15>414.15)
2:Free AMC (176.25>120.00)
3:S-320 Ac-ANW-COOH (432.20>319.15)

Ac-Ala-Asn-Trp-AMC (Ac-ANW-AMC) S-320
Ac-Ala-Asn-Trp-AMC (Ac-ANW-AMC)
By TAKASHI SHIMADA on 05/02/2020

Ordinate: fluorescent count, Em 345/Ex 445
Em band path: 9 nm
Ex band path: 20 nm
Horizontal: time [min]
Temp: 37 °C
S-320: 5, 25, 50, and 100 uM
i20S: 100 ng per assay (100 uL)
The linearity of the kinetics was confirmed, and the fluorescence intensity increased in a concentration-dependent manner. But it is considered that the saturated level has not been reached using this condition. Probably it could be a low amount of i20S.

Ac-Ala-Asn-Trp-AMC (Ac-ANW-AMC) S-320