Anti- Cetuximab (Anti-Idiotype) Antibody
Anti- Cetuximab (Anti-Idiotype) Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Cetuximab by Western Blot. Western blot shows Cetuximab, Rituximab, Brentuximab, and Gemtuzumab biosimilars. PVDF membrane was probed with 0.5 µg/mL of Anti- Cetuximab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB9626) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Cetuximab at approximately 240 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
View Larger
Detection of anti-EGFR/ErbB1 (Cetuximab) on A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with anti-EGFR/ErbB1 (Cetuximab) (Catalog # MAB9577), then anti-Cetuximab (Anti-Idiotype) (Catalog # MAB9626, filled histogram) or Normal Rabbit IgG Control (Catalog # MAB1050, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110).
View Larger
Specificity of Anti-Cetuximab (Anti-Idiotype) Antibody in Direct ELISA A panel of biosimilars was coated at 1 μg/mL in 96-well polystyrene plates. Anti-Cetuximab (Anti-Idiotype) Antibody (Catalog # MAB9626, 333 ng/mL) detected Cetuximab. No cross-reactivity with Adalimumab, Rituximab, Trastuzumab, Brentuximab, Gemtuzumab, Ibritumomab, Tositumomab, Solanezumab, Teprotumumab, and Aducanumab was observed.
View Larger
Detection of Cetuximab by Simple WesternTM. Simple Western lane view shows Cetuximab and Rituximab biosimilars, loaded at 0.2 mg/mL. A specific band was detected for Cetuximab at approximately 174 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Anti- Cetuximab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB9626). This experiment was conducted under non-reducing conditions and using the 66-440 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cetuximab
The anti-Cetuximab antibody is an anti-Idiotype antibody raised against and selected for the antigenic determinants of the variable region of Cetuximab. The antibody has been tested against a broad range of other biosimilars to ensure specificity. This highly specialized anti-idiotypic antibody can be used in a variety of pharmacokinetic (PK) assay formats.
Product Datasheets
Citation for Anti- Cetuximab (Anti-Idiotype) Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
-
Engineered CD8+ T cell-derived extracellular vesicles induce enhanced anti-cancer efficacy and targeting to lung cancer cells
Authors: Cho, H;Jung, I;Ju, H;Baek, MC;Yea, K;
Cytokine
Species: Human
Sample Types: Extracellular Vesicles
FAQs
-
What is an anti-idiotype antibody?
An anti-idiotype antibody is specific for the unique variable (antigen binding) region of another antibody. We have anti-idiotype antibodies to commecially available therapeutic antibodies or biosimilars.
Reviews for Anti- Cetuximab (Anti-Idiotype) Antibody
There are currently no reviews for this product. Be the first to review Anti- Cetuximab (Anti-Idiotype) Antibody and earn rewards!
Have you used Anti- Cetuximab (Anti-Idiotype) Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
