Anti- Cetuximab (Anti-Idiotype) PE-conjugated Antibody

Catalog #: FAB9626P Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
FAB9626P-100UG

Detection of Anti-EGFR (Cetuximab) on A431 cells by Flow Cytometry.

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All Cetuximab Antibodies

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Anti- Cetuximab (Anti-Idiotype) PE-conjugated Antibody Summary

Specificity

Detects Cetuximab in Western blots and direct ELISAs. No cross-reactivity with Adalimumab, Rituximab, Trastuzumab, Brentuximab, Gemtuzumab, Ibritumomab, Tositumomab, Solanezumab, Teprotumumab, and Aducanumab is observed.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2259C

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Full length Cetuximab

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Anti-EGFR (Cetuximab) on A431 cells by Flow Cytometry. A431 cells were stained with Human Anti-Human EGFR (Research Grade Cetuximab Biosimilar) Monoclonal Antibody (MAB9577, filled histogram) or isotype control antibody (1-001-A, open histogram), followed by Rabbit Anti-Cetuximab (Anti-Idiotype) PE monoclonal antibody (Catalog # FAB9626P). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Cetuximab

The anti-Cetuximab antibody is an anti-Idiotype antibody raised against and selected for the antigenic determinants of the variable region of Cetuximab. The antibody has been tested against a broad range of other biosimilars to ensure specificity. This highly specialized anti-idiotypic antibody can be used in a variety of pharmacokinetic (PK) assay formats.

Alternate Names

Cetuximab