Anti- Ibritumomab (Anti-Idiotype) Antibody

Catalog # Availability Size / Price Qty
MAB9544-100
MAB9544-SP
Detection of Ibritumomab by Western Blot.
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Anti- Ibritumomab (Anti-Idiotype) Antibody Summary

Specificity
Detects Ibritumomab in Western blots in direct ELISAs. In direct ELISAs, 100% cross-reactivity with Tositumomab (100% sequence identity) and no cross-reactivity with Cetuximab, Rituximab, Trastuzumab, Adalimumab, Brentuximab, Gemtuzumab, Solanezumab, Teprotumumab, and Aducanumab is observed.
Source
Monoclonal Mouse IgG2A Clone # 974622
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Full length Ibritumomab
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
ELISA
Detects Ibritumomab and Tositumomab in direct ELISA.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Ibritumomab antibody by Western Blot. View Larger

Detection of Ibritumomab by Western Blot. Western blot shows Trastuzumab, Adalimumab, and Ibritumomab biosimilars. PVDF membrane was probed with 2 µg/mL of Anti- Ibritumomab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB9544) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Ibritumomab at approximately 240 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Specificity of Anti-Ibritumomab (Anti-Idiotype) Antibody in Direct ELISA. A panel of biosimilars was coated at 1 μg/mL in 96-well polystyrene plates. Anti-Ibritumomab (Anti-Idiotype) Antibody (Catalog # MAB9544, 333 ng/mL) detected Ibritumomab. 100% cross-reactivity with Tositumomab (100% sequence identity with Ibritumomab) and no cross-reactivity with Cetuximab, Rituximab, Trastuzumab, Brentuximab, Gemtuzumab, Adalimumab, Solanezumab, Teprotumumab, and Aducanumab was observed.

Reconstitution Calculator

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ibritumomab

The anti-Ibritumomab antibody is an anti-Idiotype antibody raised against and selected for the antigenic determinants of the variable region of Ibritumomab. The antibody has been tested against a broad range of other biosimilars to ensure specificity. This highly specialized anti-idiotypic antibody can be used in a variety of pharmacokinetic (PK) assay formats.

Alternate Names
Ibritumomab

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FAQs

  1. What is an anti-idiotype antibody?

    • An anti-idiotype antibody is specific for the unique variable (antigen binding) region of another antibody.  We have anti-idiotype antibodies to commecially available therapeutic antibodies or  biosimilars.

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