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AQUApure Tri-Ub Chains (K48-linked) Protein, CF

Catalog #: UC-215B
2 Citations Datasheet / COA / SDS

Discontinued Product

UC-215B has been discontinued.
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Product Details
Citations (2)
FAQs
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Summary Product Datasheets Carrier Free Reconstitution Calculator Background Related Research Areas

AQUApure Tri-Ub Chains (K48-linked) Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

 

Ub-AQUA analysis:

K48:    99.31%

K11:      0.36%

K63:      0.19%

K6 :       0.09%

All other linkages ≤ 0.03%

Activity

Ubiquitin chains vary in length, linkage, and function. K48-linked Tri-Ubiquitin Chains (Ub3) are ideal for investigating Ubiquitin-binding proteins and as substrates for Ubiquitin-specific isopeptidases. Reaction conditions will need to be optimized for each specific application. IMPORTANT: Heating this product in SDS-PAGE buffer or terminating reactions containing this product with heated SDS-PAGE buffer could lead to unexpected, high apparent molecular weight banding or smearing on gels that is not representative of product purity. For optimal results, we recommend incubation in SDS-PAGE buffer + DTT at <40 °C for 20 minutes prior to gel electrophoresis.

Source
E. coli-derived human Tri-Ubiquitin protein
Accession #
P0CG47
Predicted Molecular Mass
26 kDa

Product Datasheets

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UC-215B

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

UC-215B

Formulation 1 mg/ml (39 μM) in sterile, deionized water
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Tri-Ubiquitin

Linkage specific Poly-Ubiquitin chains may be used as a substrate for in vitro reactions with deubiquitinating enzymes ("DUB's") that cleave the peptide or isopeptide linkage between adjacent Ubiquitin molecules. Poly-Ubiquitin chains can also be used to investigate mechanisms of binding and recognition between the chains and other proteins that contain Ubiquitin-Associated domains (UBAs), Ubiquitin-interacting motifs (UIMs), ZnF's and/or other Ubiquitin-sensing elements.

K48-linked Tri-Ubiquitin chains are manufactured using recombinant Ubiquitin and purely enzymatic techniques to avoid the potential for contaminating synthetic intermediates. The correctness of linkage and purity of each production lot is assessed using the Absolute Quantitation of Ubiquitin method (Ub-AQUA), an LCMS-based technique that provides extremely accurate information on the composition of Poly-Ubiquitin samples.

 

References
  1. Kirkpatrick D.S., et al. (2006) Nat Cell Biol. 8(7): 700-10
  2. Ordureau, A., et al. (2014) Mol. Cell  56(3): 360–375
  3. Ordureau, A., et al. (2015) Pro. Nat. Acad. of Sci. USA 112(21): 6637–6642
  4. Phu L., et al. (2011) Mol Cell Proteomics 10(5): M110.003756
Entrez Gene IDs
7314 (Human)
Alternate Names
TriUbiquitin; Tri-Ubiquitin; Ub3

Citations for AQUApure Tri-Ub Chains (K48-linked) Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. A molecular sensor determines the ubiquitin substrate specificity of SARS-CoV-2 papain-like protease
    Authors: S Patchett, Z Lv, W Rut, M Békés, M Drag, SK Olsen, TT Huang
    Cell Reports, 2021-09-08;36(13):109754.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. Translesion polymerase kappa-dependent DNA synthesis underlies replication fork recovery
    Authors: P Tonzi, Y Yin, CWT Lee, E Rothenberg, TT Huang
    Elife, 2018-11-13;7(0):.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay

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