Home / Blocking Buffers and Reagents / Bovine Serum Albumin

Bovine Serum Albumin

Catalog #: 5217
9 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
5217/100G
Bovine Serum Albumin
1 Image
Description: BSA (bovine serum albumin) is a blocking buffer for IHC, ICC, ELISA and Western blotting; used to block non-specific antibody binding. Commonly used in cell culture

Purity: ≥98%

Product Details
Citations (9)
Reviews (1)
Biological Activity Technical Data Background Product Datasheets Calculators

Biological Activity

Bovine Serum Albumin (BSA) is a widely used blocking reagent for use in immunohistochemistry (IHC), immunocytochemistry (ICC), ELISAs and Western blotting. Used to block non-specific binding of antibodies. Bovine Serum Albumin can also be used as a protein concentration standard in Bradford assay for protein quantification. BSA is also used in cell culture.

Technical Data

MW
~66 kDa
Solubility:
Soluble to 100 mg/ml in water
Purity:
≥98%
Storage:
Store at +4°C
CAS No:
9048-46-8

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

  1. Advanced glycation end products interfere with gastric smooth muscle contractile marker expression via the AGE/RAGE/NF-?B pathway
    Ting Yu
    Exp. Mol. Pathol, 2016;102(1):7-14.
  2. Nanobubbles Containing sPD-1 and Ce6 Mediate Combination Immunotherapy and Suppress Hepatocellular Carcinoma in Mice
    Y Tan, S Yang, Y Ma, J Li, Q Xie, C Liu, Y Zhao
    International Journal of Nanomedicine, 2021;16(0):3241-3254.
  3. Soluble tumor necrosis factor-alpha-induced hyperexcitability contributes to retinal ganglion cell apoptosis by enhancing Nav1.6 in experimental glaucoma
    S Cheng, HN Wang, LJ Xu, F Li, Y Miao, B Lei, X Sun, Z Wang
    Journal of Neuroinflammation, 2021;18(1):182.
  4. Rigid monoclonal antibodies improve detection of SARS-CoV-2 nucleocapsid protein
    CD Hodge, DJ Rosenberg, M Wilamowski, A Joachimiak, GL Hura, M Hammel
    bioRxiv : the preprint server for biology, 2021;0(0):.
  5. Sialyl-LewisX Glycoantigen Is Enriched on Cells with Persistent HIV Transcription during Therapy
    F Colomb, LB Giron, L Kuri-Cerva, OS Adeniji, T Ma, H Dweep, E Battivelli, E Verdin, CS Palmer, H Tateno, AV Kossenkov, NR Roan, MR Betts, M Abdel-Mohs
    Cell Rep, 2020;32(5):107991.

Product Datasheets

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Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Citations for Bovine Serum Albumin

The citations listed below are publications that use Tocris products. Selected citations for Bovine Serum Albumin include:

9 Citations: Showing 1 - 9

  1. Automated high-content imaging in iPSC-derived neuronal progenitors.
    Authors: Serena Et al.
    SLAS Discov  2023;28:42-51
  2. The developmental basis of fingerprint pattern formation and variation
    Authors: Glover Et al.
    Cell  2023;186:1
  3. SPG15 protein deficits are at the crossroads between lysosomal abnormalities, altered lipid metabolism and synaptic dysfunction.
    Authors: Pamela J Et al.
    Hum Mol Genet  2022;31:2693-2710
  4. The Circadian Clock in the Retinal Pigment Epithelium Controls the Diurnal Rhythm of Phagocytic Activity.
    Authors: P Michael Et al.
    Int J Mol Sci  2022;23
  5. Mechanosensitive expression of lamellipodin promotes intracellular stiffness, cyclin expression and cell proliferation.
    Authors: Matthias Et al.
    J Cell Sci  2021;134
  6. Mechanosensitive expression of lamellipodin promotes intracellular stiffness, cyclin expression, and cell proliferation.
    Authors: Matthias Et al.
    J Cell Sci  2021;
  7. Dopamine 2 Receptor Signaling Controls the Daily Burst in Phagocytic Activity in the Mouse Retinal Pigment Epithelium.
    Authors: David Et al.
    Invest Ophthalmol Vis Sci  2020;61:10
  8. High affinity nanobodies block SARS-CoV-2 spike receptor binding domain interaction with human angiotensin converting enzyme.
    Authors: George P Et al.
    Sci Rep  2020;10:22370
  9. Comparison of two different culture conditions for derivation of early hiPSC.
    Authors: Hanne C Et al.
    Cell Biol Int  2018;42:1467-1473

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WB blocking buffer
By Anonymous on 05/04/2021
Species: Mink

I prepared a 3% BSA solution in TBS and used this to block my membranes prior to the addition of primary antibodies as well as my diluent for my secondary antibodies.

WB blocking buffer 5217
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