Bromodeoxyuridine/BrdU APC-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Bromodeoxyuridine/BrdU in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were treated overnight with 50 ng/mL PMA, 500 ng/mL Ionomycin, and 30 µg/mL BrdU, then stained with Mouse Anti-Bromodeoxyuridine/BrdU APC-conjugated Monoclonal Antibody (Catalog # IC7225A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with cold, 70% ethanol for 5 minutes, DNA was denatured with 1.5M HCl for 30 minutes, and then cells were permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
Background: Bromodeoxyuridine/BrdU
Bromodeoxyuridine (BrdU) is a nucleoside analog that is incorporated into DNA in place of thymidine. BrdU differs from thymidine by virtue of the fact that BrdU shows a bromine atom substitution for a methyl group in thymidine. The detection of newly synthesized DNA containing BrdU is a commonly used measure of cell proliferation and progression through S phase of the cell cycle.
Product Datasheets
Citations for Bromodeoxyuridine/BrdU APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Endothelial cells are a source of Nestin expression in Pulmonary Arterial Hypertension
Authors: AR Bhagwani, S Hultman, D Farkas, R Moncayo, K Dandamudi, AK Zadu, CD Cool, L Farkas
PLoS ONE, 2019-03-18;14(3):e0213890.
Species: Human, Rat
Sample Types: Whole Cells
Applications: Flow Cytometry -
Human NK Cells Downregulate Zap70 and Syk in Response to Prolonged Activation or DNA Damage
Authors: JL Pugh, N Nemat-Gorg, PJ Norman, LA Guethlein, P Parham
J. Immunol., 2017-12-20;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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