C. botulinum BoNT-A Light Chain Antibody

Catalog #: MAB4489
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4489
MAB4489-SP

Product Details

Citations (2)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

C. botulinum BoNT-A Light Chain Antibody Summary

Species Reactivity

C. botulinum

Specificity

Detects C. botulinum BoNT‑A Light Chain in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with BoNT-B light chain, BoNT-E light chain, BoNT-A heavy chain, BoNT-C1 heavy chain, or BoNT-D heavy chain is observed.

Source

Monoclonal Mouse IgG_2A Clone # 503013

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant Clostridium BoNT-A Light Chain
Met1-Phe425
Accession # Q45894

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant C. botulinum BoNT-A Light Chain (Catalog # 4489-ZN)

Immunoprecipitation

25 µg/mL

Cell lysates spiked with Recombinant C. botulinum BoNT-A Light Chain (Catalog # 4489-ZN), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BoNT-A Light Chain

Botulinum neurotoxin type A is one of the seven serotypes of Botulinum Neurotoxins (BoNTs) produced by various strains of Clostridium botulinum (1, 2). BoNTs are synthesized as inactive single chain protein precursors and activated by proteolytic cleavage to generate disulfide-linked two-chain proteins. The 50 kDa light chain contains the catalytic domain, whereas the 100 kDa heavy chain contains an internal translocation domain and a receptor binding domain (3). BoNTs are the most potent protein toxins for humans. As zinc proteases, they cleave SNARE proteins to elicit flaccid paralysis in botulism by blocking acetylcholine release at the neuromuscular junction (2‑4). E. coli-expressed recombinant light chains are active proteases. However, they are not toxic because they cannot enter into host cells in the absence of the heavy chains.

References

Willems, A. et al. (1993) Res. Microbiol. 144:547.
Montecucco, C. and Giampietro, S. (1993) Trends. Biochem. Sci. 18:324.
Turton, K., et al. (2002) Trends. Biochem. Sci. 27:552.
Schiavo, G. et al. (2000) Physiol. Rev. 80:717.

Long Name

Botulinum Neurotoxin Type A Light Chain

Entrez Gene IDs

5185061 (C. botulinum)

Alternate Names

BoNTA Light Chain; BoNT-A Light Chain

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for C. botulinum BoNT-A Light Chain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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