Home / BoNT-A Light Chain / C. botulinum BoNT-A Light Chain Antibody

C. botulinum BoNT-A Light Chain Antibody

Catalog #: AF4489
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4489
AF4489-SP
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

C. botulinum BoNT-A Light Chain Antibody Summary

Species Reactivity
C. botulinum
Specificity
Detects C. botulinum BoNTA Light Chain in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant C. botulinum BoNTA Heavy Chain is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant Clostridium BoNT-A Light Chain
Met1-Phe425
Accession # Q45894
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Botulinum Neurotoxin Type A Light Chain (Catalog # 4489-ZN)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant C. botulinum BoNT-A Light Chain (Catalog # 4489-ZN), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BoNT-A Light Chain

Botulinum neurotoxin type A is one of the seven serotypes of Botulinum Neurotoxins (BoNTs) produced by various strains of Clostridium botulinum (1, 2). BoNTs are synthesized as inactive single chain protein precursors and activated by proteolytic cleavage to generate disulfide-linked two-chain proteins. The 50 kDa light chain contains the catalytic domain, whereas the 100 kDa heavy chain contains an internal translocation domain and a receptor binding domain (3). BoNTs are the most potent protein toxins for humans. As zinc proteases, they cleave SNARE proteins to elicit flaccid paralysis in botulism by blocking acetylcholine release at the neuromuscular junction (2-4). E. coli-expressed recombinant light chains are active proteases. However, they are not toxic because they cannot enter into host cells in the absence of the heavy chains.

References
  1. Willems, A. et al. (1993) Res. Microbiol. 144:547.
  2. Montecucco, C. and Giampietro, S. (1993) Trends. Biochem. Sci. 18:324.
  3. Turton, K., et al. (2002) Trends. Biochem. Sci. 27:552.
  4. Schiavo, G. et al. (2000) Physiol. Rev. 80:717.
Long Name
Botulinum Neurotoxin Type A Light Chain
Entrez Gene IDs
5185061 (C. botulinum)
Alternate Names
BoNTA Light Chain; BoNT-A Light Chain

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citation for C. botulinum BoNT-A Light Chain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Botulinum toxin intoxication requires retrograde transport and membrane translocation at the ER in RenVM neurons
    Authors: Yeo, JC;Tay, FP;Bennion, R;Loss, O;Maignel, J;Pons, L;Foster, K;Beard, M;Bard, F;
    eLife
    Species: Human
    Sample Types: Transduced Whole Cells
    Applications: Immunocytochemistry

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for C. botulinum BoNT-A Light Chain Antibody

There are currently no reviews for this product. Be the first to review C. botulinum BoNT-A Light Chain Antibody and earn rewards!

Have you used C. botulinum BoNT-A Light Chain Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review