C. botulinum BoNT-C1 Heavy Chain Receptor Binding Domain Antibody

Catalog # Availability Size / Price Qty
AF5425-SP Ships in 5-7 Days25 ug / $149
AF5425 In Stock100 ug / $449
Bulk Orders
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews

C. botulinum BoNT-C1 Heavy Chain Receptor Binding Domain Antibody Summary

Species Reactivity
C. botulinum
Specificity
Detects C. botulinum BoNT-C1 Heavy Chain in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross‑reactivity with recombinant (r) BoNT‑D‑HCR is observed and 5% cross-reactivity with rBoNT-A-HCR, rBoNT-A-LC, and rBoNT‑B‑LC is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant BoNT-C1 Heavy Chain
Asn866-Glu1291
Accession # P18640
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
BoNT-C1 Heavy Chain Receptor Binding Domain
Immunoprecipitation
25 µg/mL
Cell lysates spiked with Recombinant C. botulinum BoNT-C1 Heavy Chain, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BoNT-C1 Heavy Chain

BoNT-C1 (Botulinum neurotoxin C1 [heavy chain]) is a 100 kDa C-terminal fragment of the C1 Clostridum botulinum neurotoxin (holo)precursor. It constitutes a nonenzymatic part of a Zn2+-dependent M27 family endoprotease that potently blocks neurosecretion. The inactive 1291 amino acid (aa), 150 kDa C1 precursor is cleaved between Lys449Thr450 to generate a heterodimeric toxin held together by a preexisting interchain disulfide bond between Cys437 and Cys453. The 100 kDa C-terminus (heavy chain) acts as a ligand for neuronal membrane ecto-acceptors, inducing its translocation and internalization. The 50 kDa N-terminal light chain acts as an intracellular peptidase that cleaves SNAP-25 and syntaxin. This is suggested to block noradrenalin release from a readily releasable pool of vesicles. Over aa 866-1291, the C1 heavy chain shares less than 40% aa identity with the D-type neurotoxin heavy chain.                                  

Long Name
Botulinum Neurotoxin Type C1 Heavy Chain
Entrez Gene IDs
10523893 (C. botulinum)
Alternate Names
BoNTC1 Heavy Chain; BoNT-C1 Heavy Chain; DcD

Product Datasheets

You must select a language.

x

Citation for C. botulinum BoNT-C1 Heavy Chain Receptor Binding Domain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Neuronal delivery of antibodies has therapeutic effects in animal models of botulism
    Authors: Patrick M. McNutt, Edwin J. Vazquez-Cintron, Luis Tenezaca, Celinia A. Ondeck, Kyle E. Kelly, Mark Mangkhalakhili et al.
    Science Translational Medicine

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for C. botulinum BoNT-C1 Heavy Chain Receptor Binding Domain Antibody

There are currently no reviews for this product. Be the first to review C. botulinum BoNT-C1 Heavy Chain Receptor Binding Domain Antibody and earn rewards!

Have you used C. botulinum BoNT-C1 Heavy Chain Receptor Binding Domain Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review