C. botulinum BoNT-D Light Chain Biotinylated Antibody

Catalog #: BAF6037 Datasheet / COA / SDS

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BAF6037

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C. botulinum BoNT-D Light Chain Biotinylated Antibody Summary

Species Reactivity

C. botulinum

Specificity

Detects C. botulinum BoNT-D Light Chain in Western blots. In Western blots, less than 10% cross-reactivity with recombinant Light Chains of BoNT-A, -B, -C1, -E, -F, and -G is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant Clostridium BoNT-D Light Chain
Thr2-Ser428
Accession # P19321

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Botulinum Neurotoxin Type D Light Chain (Catalog # 6037-ZN)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BoNT-D Light Chain

BoNT-D LC (Botulinum neurotoxin serotype D light chain) is a 50-55 kDa member of the peptidase M27 family of molecules. It is the product of Clostridium botulinum, and inhibits acetylcholine release from neuromuscular junctions. This is accomplished by toxin internalization with subsequent cleavage of synaptobrevin, thus blocking synaptic vesicle fusion with the presynaptic membrane. Notably, BoNT-D is not toxic to human cells. BoNT/D precursor is 1276 amino acids (aa) in length. Following internalization and precursor proteolytic cleavage, it assumes a mature form that shows a disulfide-linked 442 aa light chain/enzyme N-terminus, and a 834 aa heavy chain/receptor-binding C-terminus. The 100 kDa heavy chain creates a channel within the endosome that allows for redox rupture of the disulfide bond and entry of the light chain into the cytosol. Covalent attachment of cargo molecules to the N-terminus is used to transport molecules into the cytosol. The D light chain shares less that 50% aa identity with the other botulinum serotype light chains.

Long Name

Botulinum Neurotoxin Type D Light Chain

Alternate Names

BoNTD Light Chain; BoNT-D Light Chain