C. botulinum BoNT-E Light Chain Antibody

Catalog #: AF5424 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5424
AF5424-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

C. botulinum BoNT-E Light Chain Antibody Summary

Species Reactivity

C. botulinum

Specificity

Detects C. botulinum BoNT-E Light Chain in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross‑reactivity with rBoNT-B LC is observed and less than 1% cross-reactivity with rBoNT-C HCR and rBoNT-D HCR is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant C. botulinum BoNT-E Light Chain
Pro2-Ile403
Accession # P30995

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Botulinum Neurotoxin Type E Light Chain (Catalog # 5424-ZN)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BoNT-E Light Chain

Botulinum Neurotoxin Type E is one of the seven serotypes of Botulinum Neurotoxins (BoNTs) produced by various strains of Clostridium botulinum (1, 2). BoNTs are synthesized as inactive single chain protein precursors and activated by proteolytic cleavage to generate disulfide-linked two-chain proteins. The 50 kDa light chain contains the catalytic domain, whereas the 100 kDa heavy chain contains an internal translocation domain and a receptor binding domain (3). BoNTs are the most potent protein toxins for humans. As zinc proteases, they cleave SNARE proteins to elicit flaccid paralysis in botulism by blocking acetylcholine release at the neuromuscular junction (2‑4). E. coli-expressed recombinant light chains are active proteases. In the absence of the heavy chains, however, they lack toxicity because they cannot enter into host cells.

References

Campbell K.D. et al. (1993) J. Clin. Microbiol. 31:2255.
Montecucco, C. and Giampietro, S. (1993) Trends. Biochem. Sci. 18:324.
Turton, K. et al. (2002) Trends. Biochem. Sci. 27:552.
Schiavo, G. et al. (2000) Physiol. Rev. 80:717.

Long Name

Botulinum Neurotoxin Type E Light Chain

Alternate Names

BoNTE Light Chain; BoNT-E Light Chain

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

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