Home / TeNT Light Chain / C. tetani TeNT Light Chain Antibody

C. tetani TeNT Light Chain Antibody

Catalog #: MAB6535
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB6535
MAB6535-SP
Detection ofC. tetaniTeNT Light Chain by Western Blot.
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Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

C. tetani TeNT Light Chain Antibody Summary

Species Reactivity
C. tetani
Specificity
Detects TeNT Light Chain in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant TeNT Heavy Chain is observed.
Source
Monoclonal Mouse IgG1 Clone # 604023
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant C. tetani TeNT Light Chain
Pro2-Gly430
Accession # P04958
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunoprecipitation
25 µg/mL
Cell lysates spiked with recombinant C. tetani TeNT Light Chain, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of<em>C. tetani</em>TeNT Light Chain antibody by Western Blot. View Larger

Detection ofC. tetaniTeNT Light Chain by Western Blot. Western blot shows recombinantC. tetaniTeNT Light Chain. PVDF Membrane was probed with 2 µg/mL ofC. tetaniTeNT Light Chain Monoclonal Antibody (Catalog # MAB6535) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TeNT Light Chain at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TeNT Light Chain

Tetanus toxin, a member of the peptidase M27 family of proteins, is produced by the anaerobic spore-forming bacteria Clostridium tetani as a single-chain polypeptide that is 1315 amino acids (aa) in length.  The protein is subsequently cleaved by an endogenous protease to yield the 52 kDa tetanus toxin light chain (TeNT-LC), which is 457 aa in length, and the 98 kDa tetanus toxin heavy chain that is 858 aa long.  The light and heavy chains are linked by a disulfide bridge and are non-toxic after separation.  Tetanus toxin acts by inhibiting neurotransmitter release.  It binds to peripheral neural synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons.  It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the ’76-Gln-|-Phe-77’ bond of synaptobrevin-2. 

Long Name
Tetanus Neurotoxin Type Light Chain
Entrez Gene IDs
1061100 (C. tetani)
Alternate Names
TeNT Light Chain; TeNT-LC; Tentoxylysin; Tetanus toxin chain H; Tetanus toxin chain L; Tetanus toxin heavy chain; Tetanus toxin light chain; tetX

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Citation for C. tetani TeNT Light Chain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Comparative pathogenomics of Clostridium tetani
    Authors: JE Cohen, R Wang, RF Shen, WW Wu, JE Keller
    PLoS ONE, 2017-08-11;12(8):e0182909.
    Species: Bacteria - Clostridium tetani
    Sample Types: Cell Lysates
    Applications: Western Blot

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