Calcein AM
Chemical Name: N,N'-[[3',6'-Bis(acetyloxy)-oxospiro[isobenzofuran-1-(3H),9'-(9H)xanthene]-4',5'-diyl]bis(methylene)]bis[N-[2-(acetyloxy)methoxy]-2-oxoethyl]glycine 1,1'-bis[(acetyloxy)methyl] ester
Purity: ≥90%
Biological Activity
Key information: Calcein AM is a cell permeable non-fluorescent compound, that becomes green fluorescent once hydrolyzed in live cells.Used for: cell tracing, monitoring cell viability, chemotaxis, cell adhesion and multidrug resistance.
Application: fluorescent microscopy and flow cytometry.
Properties and Photophysical Data: in live cells, non-fluorescent Calcein AM is hydrolyzed by intracellular esterases into green-fluorescent calcein, which is retained in the cytoplasm. Excitation and emission maxima (λ) are 495 nm and 515 nm, respectively.
It is recommended to prepare stock solutions of Calcein AM in DMSO.
Optical Data for Calcein AM
| Emission Color | Green |
|---|---|
| λabs | 495 nm |
| λem | 515 nm |
| Cell Permeable | Yes |
| Application | Flow Cytometry, Fluorescent Microscopy |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Background References
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Differential Modulation of Transcription Factors and Cytoskeletal Proteins in Prostate Carcinoma Cells by a Bacterial Lactone
SR Kumar, JN Bryan, AM Eaton, KL Robinson, S Gajagowni
Biomed Res Int, 2018;2018(0):6430504. -
Prostaglandin E2 activates and utilizes mTORC2 as a central signaling locus for the regulation of mast cell chemotaxis and mediator release.
Kuehn et al.
J.Biol.Chem., 2011;286:391 -
The use of calcein acetomethylester (AM)-labelled polymorphonuclear cells in a polycarbonate filter chemotaxis assay.
De Gendt et al.
Clin.Chim.Acta., 1996;249:189 -
Membrane topology and glycosylation of the human multidrug resistance-associated protein.
Bakos et al.
J.Biol.Chem., 1996;271:12322 -
Direct demonstration of mechanically induced release of cellular UTP and its implication for uridine nucleotide receptor activation.
Lazarowski et al.
J.Biol.Chem., 1997;272:24328
Product Datasheets
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Citations for Calcein AM
The citations listed below are publications that use Tocris products. Selected citations for Calcein AM include:
5 Citations: Showing 1 - 5
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Protocol for serial organoid formation assay using primary colorectal cancer tissues to evaluate cancer stem cell activity.
Authors: Yannick D Et al.
STAR Protoc 2022;3:101218
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Interleukin-1β Enhances Umbilical Cord Mesenchymal Stem Cell Adhesion Ability on Human Umbilical Vein Endothelial Cells via LFA-1/ICAM-1 Interaction.
Authors: Jiahn-Chun Et al.
Stem Cells Int 2020;2019:7267142
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Interleukin-1β induces CXCR3-mediated chemotaxis to promote umbilical cord mesenchymal stem cell transendothelial migration.
Authors: Guo Et al.
Stem Cell Res Ther 2018;9:281
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CD90 determined two subpopulations of glioma-associated mesenchymal stem cells with different roles in tumour progression.
Authors: Zhang Et al.
Cell Death Dis 2018;9:1101
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Loss of the mitochondrial kinase PINK1 does not alter platelet function.
Authors: Walsh Et al.
Sci Rep 2018;8:14377
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