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cAMP Parameter Assay Kit

Catalog #: KGE002B
108 Citations Datasheet / COA / SDS
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QC52: Parameter Immunoassay Control Set 1165 for cAMP
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Multi-species cAMP ELISA Cell Lysis Buffer 5 (1X) Standard Curve
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Citations (108)
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

cAMP Parameter Assay Kit Summary

Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Cell Lysates (100 uL), Serum (20 uL), EDTA Plasma (20 uL), Heparin Plasma (20 uL), Saliva (50 uL), Urine (10 uL)
Sensitivity
8.57 pmol/mL
Assay Range
3.8 - 240 pmol/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
Specificity
Measures cyclic AMP levels in various samples
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Parameter cAMP Immunoassay is a 3.5 hour competitive enzyme immunoassay designed to measure cAMP in cell culture supernates, cell lysates, plasma, serum, saliva, and urine.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pmol/mL) 22.6 68.7 116 21.7 70.4 119
Standard Deviation 1.37 2.55 2.54 2.2 6 7.3
CV% 6 3.7 2.2 10.3 8.6 6.1

Cell Lysates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pmol/mL) 18.5 56.9 96.1 17 51.8 89.2
Standard Deviation 0.89 1.74 2.73 1.3 3.5 6.9
CV% 4.8 3.1 2.8 7.4 6.8 7.7

Recovery

The recovery of cAMP spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 104 86-119
Cell Lysis Buffer (n=1) 91 87-94
EDTA Plasma (n=4) 105 92-120
Heparin Plasma (n=4) 98 85-111
Saliva (n=4) 97 80-117
Serum (n=4) 103 97-114
Urine (n=6) 100 91-119

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of cAMP were serially diluted with the appropriate diluent to produce samples with values within the dynamic range of the assay.
Multi-species cAMP ELISA Linearity

Scientific Data

N/A Multi-species cAMP ELISA Cell Lysis Buffer 5 (1X) Standard Curve View Larger

Multi-species cAMP ELISA Cell Lysis Buffer 5 (1X) Standard Curve

N/A Multi-species cAMP ELISA Calibrator Diluent RD5-55 Standard Curve View Larger

Multi-species cAMP ELISA Calibrator Diluent RD5-55 Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: cAMP

Adenosine 3,5-cyclic monophosphate (cAMP) is one of the most important intracellular second messengers. Several hormones are known to activate cAMP and subsequent signaling cascades via their indirect influence on adenylate cyclase, the enzyme responsible for the conversion of ATP to cAMP.

Long Name:
Cyclic AMP
Alternate Names:
cAMP; Cyclic AMP
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for cAMP Parameter Assay Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

108 Citations: Showing 1 - 10
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  1. Phosphoproteomics Reveals Selective Regulation of Signaling Pathways by Lysophosphatidic Acid Species in Macrophages
    Authors: Dietze, R;Szymanski, W;Ojasalu, K;Finkernagel, F;Nist, A;Stiewe, T;Graumann, J;Müller, R;
    Cells
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Comparative analysis of SEC61A1 mutant R236C in two patient-derived cellular platforms
    Authors: Weiand, M;Sandfort, V;Nadzemova, O;Schierwagen, R;Trebicka, J;Schlevogt, B;Kabar, I;Schmidt, H;Zibert, A;
    Scientific reports
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Destruction of self-derived PAMP via T3SS2 effector VopY to subvert PAMP-triggered immunity mediates Vibrio parahaemolyticus pathogenicity
    Authors: Wu, X;Zhou, L;Ye, C;Zha, Z;Li, C;Feng, C;Zhang, Y;Jin, Q;Pan, J;
    Cell reports
    Species: Human
    Sample Types: Cell Culture Supernates
  4. A Side-by-Side Comparison of Wildtype and Variant Melanocortin 1 Receptor Signaling with Emphasis on Protection against Oxidative Damage to DNA
    Authors: Cerdido, S;Sánchez-Beltrán, J;Lambertos, A;Abrisqueta, M;Padilla, L;Herraiz, C;Olivares, C;Jiménez-Cervantes, C;García-Borrón, JC;
    International journal of molecular sciences
    Species: Human
    Sample Types: Cell Lysates
  5. EPs® 7630 Stimulates Tissue Repair Mechanisms and Modifies Tight Junction Protein Expression in Human Airway Epithelial Cells
    Authors: Fang, L;Zhou, L;Kulić, Ž;Lehner, MD;Tamm, M;Roth, M;
    International journal of molecular sciences
    Species: Human
    Sample Types: Cell Lysates
  6. Butyrate dictates ferroptosis sensitivity through FFAR2-mTOR signaling
    Authors: Wang, G;Qin, S;Chen, L;Geng, H;Zheng, Y;Xia, C;Yao, J;Deng, L;
    Cell death & disease
    Species: Human
    Sample Types: Cell Lysates
  7. An Anaplasma phagocytophilum T4SS effector, AteA, is essential for tick infection
    Authors: JM Park, BM Genera, D Fahy, KT Swallow, CM Nelson, JD Oliver, DK Shaw, UG Munderloh, KA Brayton
    bioRxiv : the preprint server for biology, 2023-02-06;0(0):.
    Species: Complex Species Category
    Sample Types: Cell Lysates
  8. Both LTA and LTB Subunits Are Equally Important to Heat-Labile Enterotoxin (LT)-Enhanced Bacterial Adherence
    Authors: Q Duan, S Pang, L Feng, B Li, L Lv, Y Liang, G Zhu
    International Journal of Molecular Sciences, 2023-01-08;24(2):.
    Species: Bacteria, Porcine
    Sample Types: Cell Culture Supernates
  9. Identification and Pharmacological Characterization of Two Serotonin Type 7 Receptor Isoforms from Mythimna separata
    Authors: W Chen, X Gao, H Wang, G Xie, S An, Y Du, X Zhao
    International Journal of Molecular Sciences, 2022-12-30;24(1):.
    Species: Insect-Oriental Armyworm
    Sample Types: Cell Lysates
  10. Prostacyclin Released by Cancer-Associated Fibroblasts Promotes Immunosuppressive and Pro-Metastatic Macrophage Polarization in the Ovarian Cancer Microenvironment
    Authors: L Sommerfeld, I Knuth, F Finkernage, J Pesek, WA Nockher, JM Jansen, U Wagner, A Nist, T Stiewe, S Müller-Brü, R Müller, S Reinartz
    Cancers, 2022-12-13;14(24):.
    Species: Human
    Sample Types: Cell Lysates
  11. Natural statin derivatives as potential therapy to reduce intestinal fluid loss in cholera
    Authors: R Noitem, P Pongkorpsa, C Changsen, Y Sukpondma, C Tansakul, V Rukachaisi, C Muanprasat
    PloS Neglected Tropical Diseases, 2022-12-09;16(12):e0010989.
    Species: Human
    Sample Types: Cell Lysates
  12. SNA077, an Extract of Marine Streptomyces sp., Inhibits Melanogenesis by Downregulating Melanogenic Proteins via Inactivation of cAMP/PKA/CREB Signaling
    Authors: SJ Lim, DW Jung, PF Hillman, SJ Nam, CS Lee
    International Journal of Molecular Sciences, 2022-11-29;23(23):.
    Species: Human
    Sample Types: Cell Lysates
  13. Adult human kidney organoids originate from CD24+ cells and represent an advanced model for adult polycystic kidney disease
    Authors: Y Xu, C Kuppe, J Perales-Pa, S Hayat, J Kranz, AT Abdallah, J Nagai, Z Li, F Peisker, T Saritas, M Halder, S Menzel, K Hoeft, A Kenter, H Kim, CRC van Roeyen, M Lehrke, J Moellmann, T Speer, EM Buhl, R Hoogenboez, P Boor, J Jansen, C Knopp, I Kurth, B Smeets, E Bindels, MEJ Reinders, C Baan, J Gribnau, EJ Hoorn, J Steffens, TB Huber, I Costa, J Floege, RK Schneider, J Saez-Rodri, BS Freedman, R Kramann
    Nature Genetics, 2022-10-27;0(0):.
    Species: Human
    Sample Types: Cell Lysates
  14. Short-Chain Fatty Acids Modulate Sperm Migration through Olfactory Receptor 51E2 Activity
    Authors: E Teveroni, F Di Nicuolo, E Vergani, C Bruno, G Maulucci, G Bianchetti, AL Astorri, G Grande, J Gervasoni, L Santucci, M De Spirito, A Urbani, A Pontecorvi, F Mancini, D Milardi
    International Journal of Molecular Sciences, 2022-10-22;23(21):.
    Species: Human
    Sample Types: Cell Lysates
  15. G protein-biased GPR3 signaling ameliorates amyloid pathology in a preclinical Alzheimer's disease mouse model
    Authors: Y Huang, T Rafael Gui, N Todd, C Ferguson, KM Weiss, FR Stauffer, B McDermott, BT Hurtle, T Saito, TC Saido, ML MacDonald, GE Homanics, A Thathiah
    Proceedings of the National Academy of Sciences of the United States of America, 2022-09-26;119(40):e2204828119.
    Species: Chinese Hamster
    Sample Types: Whole Cells
  16. Pharmacological Properties and Function of the PxOctbeta3 Octopamine Receptor in Plutella xylostella (L.)
    Authors: H Zhu, Z Liu, H Ma, W Zheng, J Liu, Y Zhou, Y Man, X Zhou, A Zeng
    Insects, 2022-08-16;13(8):.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. Subversion of GBP-mediated host defense by E3 ligases acquired during Yersinia pestis evolution
    Authors: S Cao, Y Jiao, W Jiang, Y Wu, S Qin, Y Ren, Y You, Y Tan, X Guo, H Chen, Y Zhang, G Wu, T Wang, Y Zhou, Y Song, Y Cui, F Shao, R Yang, Z Du
    Nature Communications, 2022-08-04;13(1):4526.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. Novel CFTR Activator Cact-3 Ameliorates Ocular Surface Dysfunctions in Scopolamine-Induced Dry Eye Mice
    Authors: D Jeon, I Jun, HK Lee, J Park, BR Kim, K Ryu, H Yoon, TI Kim, W Namkung
    International Journal of Molecular Sciences, 2022-05-06;23(9):.
    Species: Hamster
    Sample Types: Cell Culture Supernates
  19. Progesterone activates GPR126 to promote breast cancer development via the Gi pathway
    Authors: W An, H Lin, L Ma, C Zhang, Y Zheng, Q Cheng, C Ma, X Wu, Z Zhang, Y Zhong, M Wang, D He, Z Yang, L Du, S Feng, C Wang, F Yang, P Xiao, P Zhang, X Yu, JP Sun
    Proceedings of the National Academy of Sciences of the United States of America, 2022-04-08;119(15):e2117004119.
    Species: Human
    Sample Types: Cell Culture Supernates
  20. The effect of radioiodine treatment on the characteristics of TRAb in Graves' disease
    Authors: Y Fang, WH Du, CX Zhang, SX Zhao, HD Song, GQ Gao, M Dong
    BMC endocrine disorders, 2021-11-30;21(1):238.
    Species: Chinese Hamster
    Sample Types: Cell Culture Supernates
  21. TSH Combined with TSHR Aggravates Diabetic Peripheral Neuropathy by Promoting Oxidative Stress and Apoptosis in Schwann Cells
    Authors: J Fan, Q Pan, Q Gao, W Li, F Xiao, L Guo
    Oxidative Medicine and Cellular Longevity, 2021-11-11;2021(0):2482453.
    Species: Rat
    Sample Types: Cell Lysates
  22. Two ACTH analogs exert differential effects on monocytes/macrophages function regulation in ayu (Plecoglossus altivelis)
    Authors: LL Feng, YW Dai, XJ Lu, JF Lu, GJ Yang, H Zhang, L Zhang, J Chen
    General and comparative endocrinology, 2021-04-24;0(0):113796.
    Species: Ayu (Plecoglossus altivelis)
    Sample Types: Cell Lysates
  23. Repurposing calcium sensing receptor agonist cinacalcet for treatment of CFTR-mediated secretory diarrheas
    Authors: AA Oak, PD Chhetri, A Rivera, AS Verkman, O Cil
    JCI Insight, 2021-02-22;0(0):.
    Species: Human
    Sample Types: Cell Lysates
  24. Unraveling the developmental roadmap toward human brown adipose tissue
    Authors: S Carobbio, AC Guenantin, M Bahri, S Rodriguez-, F Honig, I Kamzolas, I Samuelson, K Long, S Awad, D Lukovic, S Erceg, A Bassett, S Mendjan, L Vallier, BS Rosen, D Chiarugi, A Vidal-Puig
    Stem Cell Reports, 2021-02-18;16(3):641-655.
    Species: Human
    Sample Types: Cell Culture Supernates
  25. Site-selective modification of exendin 4 with variable molecular weight dextrans by oxime-ligation chemistry for improving type 2 diabetic treatment
    Authors: S Dai, S Liu, C Li, Z Zhou, Z Wu
    Carbohydr Polym, 2020-08-07;249(0):116864.
    Species: Human
    Sample Types: Cell Lysates
  26. Calcitonin gene-related peptide inhibits angiotensin II-induced NADPH oxidase-dependent ROS via the Src/STAT3 signalling pathway
    Authors: HM Luo, X Wu, X Xian, LY Wang, LY Zhu, HY Sun, L Yang, WX Liu
    J. Cell. Mol. Med., 2020-05-05;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
  27. Recombinant Peptide Production Platform Coupled with Site-Specific Albumin Conjugation Enables a Convenient Production of Long-Acting Therapeutic Peptide
    Authors: M Bak, J Park, K Min, J Cho, J Seong, YS Hahn, G Tae, I Kwon
    Pharmaceutics, 2020-04-16;12(4):.
    Species: Human
    Sample Types: Cell Lysates
  28. Glutathione dynamics determine the therapeutic efficacy of mesenchymal stem cells for graft-versus-host disease via CREB1-NRF2 pathway
    Authors: J Lim, J Heo, H Ju, JW Shin, Y Kim, S Lee, HY Yu, CM Ryu, H Yun, S Song, KS Hong, HM Chung, HR Kim, JS Roe, K Choi, IG Kim, EM Jeong, DM Shin
    Sci Adv, 2020-04-15;6(16):eaba1334.
    Species: Human
    Sample Types: Cell Lysates
  29. mPGES-1/PGE2 promotes the growth of T-ALL cells in vitro and in vivo by regulating the expression of MTDH via the EP3/cAMP/PKA/CREB pathway
    Authors: Y Li, J Chen, W Yang, H Liu, J Wang, J Xiao, S Xie, L Ma, D Nie
    Cell Death Dis, 2020-04-06;11(4):221.
    Species: Human
    Sample Types: Cell Culture Supernates
  30. Molecular and pharmacological characterization of a &beta-adrenergic-like octopamine receptor from the green rice leafhopper Nephotettix cincticeps
    Authors: G Xu, XF Chang, GX Gu, WX Jia, L Guo, J Huang, GY Ye
    Insect Biochem. Mol. Biol., 2020-02-25;0(0):103337.
    Species: Chinese Hamster
    Sample Types: Whole Cells
  31. &beta2-Adrenergic Signalling Promotes Cell Migration by Upregulating Expression of the Metastasis-Associated Molecule LYPD3
    Authors: M Gruet, D Cotton, C Coveney, DJ Boocock, S Wagner, L Komorowski, RC Rees, AG Pockley, AC Garner, JD Wallis, AK Miles, DG Powe
    Biology (Basel), 2020-02-22;9(2):.
    Species: Human
    Sample Types: Cell Culture Supernates
  32. The Adenosine A2B Receptor Drives Osteoclast-Mediated Bone Resorption in Hypoxic Microenvironments
    Authors: HJ Knowles
    Cells, 2019-06-21;8(6):.
    Species: Human
    Sample Types: Cell Lysates
  33. Intestinal epithelial potassium channels and CFTR chloride channels activated in ErbB tyrosine kinase inhibitor diarrhea
    Authors: T Duan, O Cil, JR Thiagaraja, AS Verkman
    JCI Insight, 2019-02-21;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  34. Adrenomedullin mediates pro-angiogenic and pro-inflammatory cytokines in asthma and COPD
    Authors: J Mandal, M Roth, E Papakonsta, L Fang, S Savic, M Tamm, D Stolz
    Pulm Pharmacol Ther, 2019-01-25;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  35. Toxicity and possible mechanisms of action of honokiol from Magnolia denudata seeds against four mosquito species
    Authors: Z Wang, H Perumalsam, X Wang, YJ Ahn
    Sci Rep, 2019-01-23;9(1):411.
    Species: Insect - Yellow Fever Mosquito
    Sample Types: Tissue Homogenates
  36. Glucagon-related peptides from phylogenetically ancient fish reveal new approaches to the development of dual GCGR and GLP1R agonists for type 2 diabetes therapy
    Authors: GV Graham, JM Conlon, YH Abdel-Waha, PR Flatt
    Peptides, 2018-11-01;110(0):19-29.
    Species: Chinese Hamster
    Sample Types: Cell Lysates
  37. ADRB2 polymorphism Arg16Gly modifies the natural outcome of heart failure and dictates therapeutic response to ?-blockers in patients with heart failure
    Authors: J Huang, C Li, Y Song, X Fan, L You, L Tan, L Xiao, Q Li, G Ruan, S Hu, W Cui, Z Li, L Ni, C Chen, AY Woo, RP Xiao, DW Wang
    Cell Discov, 2018-10-23;4(0):57.
    Species: Human
    Sample Types: Cell Lysates
  38. Senescent fibroblasts drive ageing pigmentation: ?A potential therapeutic target for senile lentigo
    Authors: JE Yoon, Y Kim, S Kwon, M Kim, YH Kim, JH Kim, TJ Park, HY Kang
    Theranostics, 2018-09-09;8(17):4620-4632.
    Species: Human
    Sample Types: Cell Lysates
  39. Salmonella Typhimurium effector SseI inhibits chemotaxis and increases host cell survival by deamidation of heterotrimeric Gi proteins
    Authors: T Brink, V Leiss, P Siegert, D Jehle, JK Ebner, C Schwan, A Shymanets, S Wiese, B Nürnberg, M Hensel, K Aktories, JHC Orth
    PLoS Pathog., 2018-08-13;14(8):e1007248.
    Species: Human
    Sample Types: Cell Lysates
  40. Sera from Septic Patients Contain the Inhibiting Activity of the Extracellular ATP-Dependent Inflammasome Pathway
    Authors: VM Ho, N Hirohashi, WS Kong, G Yun, K Ota, J Itai, S Yamaga, K Suzuki, K Tanigawa, M Kanno, N Shime
    Tohoku J. Exp. Med., 2018-07-01;245(3):193-204.
    Species: Human
    Sample Types: Cell Culture Supernates
  41. Human evolutionary loss of epithelial Neu5Gc expression and species-specific susceptibility to cholera
    Authors: F Alisson-Si, JZ Liu, SL Diaz, L Deng, MG Gareau, R Marchellet, X Chen, V Nizet, N Varki, KE Barrett, A Varki
    PLoS Pathog., 2018-06-18;14(6):e1007133.
    Species: Human
    Sample Types: Cell Lysates
  42. Heteromerization of ?-opioid receptor and cholecystokinin B receptor through the third transmembrane domain of the ?-opioid receptor contributes to the anti-opioid effects of cholecystokinin octapeptide
    Authors: Y Yang, Q Li, QH He, JS Han, L Su, Y Wan
    Exp. Mol. Med., 2018-05-21;50(5):64.
    Species: Human
    Sample Types: Cell Lysates
  43. Anticoagulant mechanism, pharmacological activity, and assessment of preclinical safety of a novel fibrin(ogen)olytic serine protease from leaves of Leucas indica
    Authors: D Gogoi, N Arora, B Kalita, R Sarma, T Islam, SS Ghosh, R Devi, AK Mukherjee
    Sci Rep, 2018-04-18;8(1):6210.
    Species: Human
    Sample Types: Cell Culture Supernates
  44. A cAMP/PKA/Kinesin-1 Axis Promotes the Axonal Transport of Mitochondria in Aging Drosophila Neurons
    Authors: A Vagnoni, SL Bullock
    Curr. Biol., 2018-03-29;0(0):.
    Species: Drosophila
    Sample Types: Tissue Culture Supernates
  45. Apelin-13 analogues show potent in vitro and in vivo insulinotropic and glucose lowering actions
    Authors: FPM O'Harte, V Parthsarat, C Hogg, PR Flatt
    Peptides, 2018-02-01;100(0):219-228.
    Species: Rat
    Sample Types: Cell Lysates
  46. Ovicidal and larvicidal activity and possible mode of action of phenylpropanoids and ketone identified in Syzygium aromaticum bud against Bradysia procera
    Authors: TK Hong, H Perumalsam, KH Jang, ES Na, YJ Ahn
    Pestic Biochem Physiol, 2018-01-11;145(0):29-38.
    Species: Insect - Bradysia procera
    Sample Types: Tissue Homogenates
  47. Molecular and functional characterization of tumor-induced factor (TIF): Hamster homolog of CXCL3 (GRO?) displays tumor suppressive activity
    Authors: L Jin, ZF Li, DK Wang, M Sun, W Qi, Q Ma, L Zhang, C Chu, EYM Chan, SST Lee, H Wise, KF To, Y Shi, N Zhou, WT Cheung
    Cytokine, 2017-12-23;102(0):62-75.
    Species: Human
    Sample Types: Cell Culture Supernates
  48. Mitochondrial Abnormality Facilitates Cyst Formation in Autosomal Dominant Polycystic Kidney Disease
    Authors: Y Ishimoto, R Inagi, D Yoshihara, M Kugita, S Nagao, A Shimizu, N Takeda, M Wake, K Honda, J Zhou, M Nangaku
    Mol. Cell. Biol., 2017-11-28;0(0):.
    Species: Human
    Sample Types: Cell Lysates
  49. Intermedin Ameliorates Atherosclerosis by Increasing Cholesterol Efflux Through the cAMP-PKA Pathway in Macrophage RAW264.7 Cell Line
    Authors: H Liao, S Wan, X Zhang, D Shi, X Zhu, X Chen
    Med. Sci. Monit., 2017-11-17;23(0):5462-5471.
    Species: Human, Mouse
    Sample Types: Cell Lysates
  50. Gpr158 mediates osteocalcin's regulation of cognition
    Authors: L Khrimian, A Obri, M Ramos-Bros, A Rousseaud, S Moriceau, AS Nicot, P Mera, S Kosmidis, T Karnavas, F Saudou, XB Gao, F Oury, E Kandel, G Karsenty
    J. Exp. Med., 2017-08-29;214(10):2859-2873.
    Species: Mouse
    Sample Types: Cell Lysates
  51. BNGR-A25L and -A27 are two functional G protein-coupled receptors for CAPA periviscerokinin neuropeptides in the silkworm, Bombyx mori
    Authors: Z Shen, Y Chen, L Hong, Z Cui, H Yang, X He, Y Shi, L Shi, F Han, N Zhou
    J. Biol. Chem., 2017-08-24;0(0):.
    Species: Human
    Sample Types: Cell Lysates
  52. Membrane-Associated Effects of Glucocorticoid on BACE1 Upregulation and A? Generation: Involvement of Lipid Raft-Mediated CREB Activation
    Authors: GE Choi, SJ Lee, HJ Lee, SH Ko, CW Chae, HJ Han
    J. Neurosci., 2017-08-03;37(35):8459-8476.
    Species: Human
    Sample Types: Cell Lysates
  53. Caffeine induces gastric acid secretion via bitter taste signaling in gastric parietal cells
    Authors: KI Liszt, JP Ley, B Lieder, M Behrens, V Stöger, A Reiner, CM Hochkogler, E Köck, A Marchiori, J Hans, S Widder, G Krammer, GJ Sanger, MM Somoza, W Meyerhof, V Somoza
    Proc. Natl. Acad. Sci. U.S.A., 2017-07-10;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  54. Characterization of three serotonin receptors from the small white butterfly, Pieris rapae
    Authors: YX Qi, M Jin, XY Ni, GY Ye, Y Lee, J Huang
    Insect Biochem. Mol. Biol., 2017-06-27;0(0):.
    Species: Human
    Sample Types: Cell Lysates
  55. Pharmacological characterisation and functional roles for egg-laying of a ?-adrenergic-like octopamine receptor in the brown planthopper Nilaparvata lugens
    Authors: SF Wu, XM Jv, J Li, GJ Xu, XY Cai, CF Gao
    Insect Biochem. Mol. Biol., 2017-06-16;87(0):55-64.
    Species: Human
    Sample Types: Cell Lysates
  56. Protein kinase A-mediated phosphorylation of the Broad-Complex transcription factor in silkworm suppresses its transcriptional activity
    Authors: W Qian, X Gang, T Zhang, L Wei, X Yang, Z Li, Y Yang, L Song, P Wang, J Peng, D Cheng, Q Xia
    J. Biol. Chem., 2017-06-05;0(0):.
    Species: Silkworm
    Sample Types: Cell Culture Supernates
  57. The neuropeptide F/nitric oxide pathway is essential for shaping locomotor plasticity underlying locust phase transition
    Authors: L Hou, P Yang, F Jiang, Q Liu, X Wang, L Kang
    Elife, 2017-03-27;6(0):.
    Species: Insect - Locust
    Sample Types: Tissue Homogenates
  58. Pharmacological characterization of dopamine receptors in the rice striped stem borer, Chilo suppressalis
    Authors: G Xu, SF Wu, GX Gu, ZW Teng, GY Ye, J Huang
    Insect Biochem. Mol. Biol, 2017-03-14;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  59. E-Prostanoid 3 Receptor Mediates Sprouting Angiogenesis Through Suppression of the Protein Kinase A/?-Catenin/Notch Pathway
    Authors: D Chen, J Tang, Q Wan, J Zhang, K Wang, Y Shen, Y Yu
    Arterioscler. Thromb. Vasc. Biol, 2017-03-02;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
  60. 5-HTR3 and 5-HTR4 located on the mitochondrial membrane and functionally regulated mitochondrial functions
    Sci Rep, 2016-11-22;6(0):37336.
    Species: Mouse
    Sample Types: Cell Lysates
  61. Endothelial PDGF-CC regulates angiogenesis-dependent thermogenesis in beige fat
    Nat Commun, 2016-08-05;7(0):12152.
    Species: Mouse
    Sample Types: Tissue Homogenates
  62. Functional Characterization of the Odorant Receptor 51E2 in Human Melanocytes
    J Biol Chem, 2016-05-18;0(0):.
    Species: Human
    Sample Types: Cell Lysates
  63. Molecular Characterization of E-Type Prostanoid Receptor 4 (EP4) from Ayu (Plecoglossus altivelis) and Its Functional Analysis in the Monocytes/Macrophages.
    Authors: Rong Y, Lu X, Chen J
    PLoS ONE, 2016-01-25;11(1):e0147884.
    Species: Fish - Plecoglossus altivelis (Ayu)
    Sample Types: Cell Lysates
  64. Evaluating the A-Subunit of the Heat-Labile Toxin (LT) As an Immunogen and a Protective Antigen Against Enterotoxigenic Escherichia coli (ETEC).
    Authors: Norton E, Branco L, Clements J
    PLoS ONE, 2015-08-25;10(8):e0136302.
    Species: Human
    Sample Types: Cell Lysates
  65. Formaldehyde Crosses the Human Placenta and Affects Human Trophoblast Differentiation and Hormonal Functions.
    Authors: Pidoux G, Gerbaud P, Guibourdenche J, Therond P, Ferreira F, Simasotchi C, Evain-Brion D, Gil S
    PLoS ONE, 2015-07-17;10(7):e0133506.
    Species: Human
    Sample Types: Whole Cells
  66. The stress response neuropeptide CRF increases amyloid-beta production by regulating gamma-secretase activity.
    Authors: Park H, Ran Y, Jung J, Holmes O, Price A, Smithson L, Ceballos-Diaz C, Han C, Wolfe M, Daaka Y, Ryabinin A, Kim S, Hauger R, Golde T, Felsenstein K
    EMBO J, 2015-05-11;34(12):1674-86.
    Species: Human
    Sample Types: Cell Lysates
  67. Sequence analysis and identification of new isoform of EP4 receptors in different atlantic salmon tissues (Salmo salar L.) and its role in PGE2 induced immunomodulation in vitro.
    Authors: Guo T, Gamil A, Koenig M, Evensen O
    PLoS ONE, 2015-04-02;10(3):e0120483.
    Species: Fish - Atlantic Salmon, Fish - Oncorhynchus mykiss (Rainbow Trout), Fish - Oncorhynchus tshawytscha (Chinook Salmon)
    Sample Types: Cell Lysates
  68. ASM-024, a piperazinium compound, promotes the in vitro relaxation of beta2-adrenoreceptor desensitized tracheas.
    Authors: Israel-Assayag E, Beaulieu M, Cormier Y
    PLoS ONE, 2015-03-23;10(3):e0120095.
    Species: Human
    Sample Types: Cell Culture Supernates
  69. beta-Hydroxybutyric sodium salt inhibition of growth hormone and prolactin secretion via the cAMP/PKA/CREB and AMPK signaling pathways in dairy cow anterior pituitary cells.
    Authors: Fu S, Wang W, Liu B, Yang H, Ji H, Yang Z, Guo B, Liu J, Wang J
    Int J Mol Sci, 2015-02-16;16(2):4265-80.
    Species: Bovine
    Sample Types: Cell Culture Supernates
  70. Simultaneous exposure to Escherichia coli heat-labile and heat-stable enterotoxins increases fluid secretion and alters cyclic nucleotide and cytokine production by intestinal epithelial cells.
    Authors: Read L, Hahn R, Thompson C, Bauer D, Norton E, Clements J
    Infect Immun, 2014-10-06;82(12):5308-16.
    Species: Human
    Sample Types: Cell Lysates
  71. Cannabidiol exerts sebostatic and antiinflammatory effects on human sebocytes.
    Authors: Olah A, Toth B, Borbiro I, Sugawara K, Szollosi A, Czifra G, Pal B, Ambrus L, Kloepper J, Camera E, Ludovici M, Picardo M, Voets T, Zouboulis C, Paus R, Biro T
    J Clin Invest, 2014-07-25;124(9):3713-24.
    Species: Human
    Sample Types: Cell Lysates
  72. Intermittent PTHrP(1-34) exposure augments chondrogenesis and reduces hypertrophy of mesenchymal stromal cells.
    Authors: Fischer, Jennifer, Aulmann, Antje, Dexheimer, Verena, Grossner, Tobias, Richter, Wiltrud
    Stem Cells Dev, 2014-07-02;23(20):2513-23.
    Species: Human
    Sample Types: Cell Culture Supernates
  73. Constitutive Galphai coupling activity of very large G protein-coupled receptor 1 (VLGR1) and its regulation by PDZD7 protein.
    Authors: Hu Q, Dong J, Du H, Zhang D, Ren H, Ma M, Cai Y, Zhao T, Yin X, Yu X, Xue T, Xu Z, Sun J
    J Biol Chem, 2014-06-24;289(35):24215-25.
    Species: Human
    Sample Types: Cell Culture Supernates
  74. Apremilast is a selective PDE4 inhibitor with regulatory effects on innate immunity.
    Authors: Schafer P, Parton A, Capone L, Cedzik D, Brady H, Evans J, Man H, Muller G, Stirling D, Chopra R
    Cell Signal, 2014-05-29;26(9):2016-29.
    Species: Human
    Sample Types: Cell Culture Supernates
  75. Activation of glucagon-like peptide-1 receptor inhibits growth and promotes apoptosis of human pancreatic cancer cells in a cAMP-dependent manner.
    Authors: Zhao H, Wei R, Wang L, Tian Q, Tao M, Ke J, Liu Y, Hou W, Zhang L, Yang J, Hong T
    Am J Physiol Endocrinol Metab, 2014-05-06;306(12):E1431-41.
    Species: Human
    Sample Types: Whole Cells
  76. Exemestane blocks mesothelioma growth through downregulation of cAMP, pCREB and CD44 implicating new treatment option in patients affected by this disease.
    Authors: Nuvoli, Barbara, Germoni, Sabrina, Morosetti, Carlotta, Santoro, Raffaela, Cortese, Giancarl, Masi, Serena, Cordone, Iole, Galati, Rossella
    Mol Cancer, 2014-03-21;13(0):69.
    Species: Human
    Sample Types: Cell Lysates
  77. Antidiarrheal efficacy and cellular mechanisms of a Thai herbal remedy.
    Authors: Tradtrantip L, Ko E, Verkman A
    PLoS Negl Trop Dis, 2014-02-13;8(2):e2674.
    Species: Human
    Sample Types: Cell Culture Supernates
  78. Low adiposity during early infancy is associated with a low risk for developing dengue hemorrhagic fever: a preliminary model.
    Authors: Libraty D, Zhang L, Woda M, Giaya K, Kathivu C, Acosta L, Tallo V, Segubre-Mercado E, Bautista A, Obcena A, Brion J, Capeding R
    PLoS ONE, 2014-02-12;9(2):e88944.
    Species: Human
    Sample Types: Cell Culture Supernates
  79. Cardiac progenitor cells engineered with betaARKct have enhanced beta-adrenergic tolerance.
    Authors: Khan M, Mohsin S, Toko H, Alkatib M, Nguyen J, Truffa S, Gude N, Chuprun K, Tilley D, Koch W, Sussman M
    Mol Ther, 2013-09-03;22(1):178-85.
    Species: Human
    Sample Types: Cell Lysates
  80. Role of tyrosine phosphorylation in the muscarinic activation of the cystic fibrosis transmembrane conductance regulator (CFTR).
    Authors: Billet A, Luo Y, Balghi H, Hanrahan J
    J Biol Chem, 2013-06-11;288(30):21815-23.
    Species: Hamster
    Sample Types: Cell Lysates
  81. An siRNA screen identifies the GNAS locus as a driver in 20q amplified breast cancer.
    Authors: Garcia-Murillas I, Sharpe R, Pearson A, Campbell J, Natrajan R, Ashworth A, Turner N
    Oncogene, 2013-06-10;33(19):2478-86.
    Species: Human
    Sample Types: Cell Culture Supernates
  82. Bioengineered 3D human kidney tissue, a platform for the determination of nephrotoxicity.
    Authors: DesRochers, Teresa M, Suter, Laura, Roth, Adrian, Kaplan, David L
    PLoS ONE, 2013-03-14;8(3):e59219.
    Species: Human
    Sample Types: Cell Culture Supernates
  83. Specific activation of the G protein-coupled receptor BNGR-A21 by the neuropeptide corazonin from the silkworm, Bombyx mori, dually couples to the G(q) and G(s) signaling cascades.
    Authors: Yang, Jingwen, Huang, Haishan, Yang, Huipeng, He, Xiaobai, Jiang, Xue, Shi, Ying, Alatangaole, Damirin, Shi, Liangen, Zhou, Naiming
    J Biol Chem, 2013-03-01;288(17):11662-75.
    Species: Human, Moth - Spodoptera
    Sample Types: Cell Culture Supernates
  84. Amyloid beta (Abeta) peptide directly activates amylin-3 receptor subtype by triggering multiple intracellular signaling pathways.
    Authors: Fu W, Ruangkittisakul A, MacTavish D, Shi JY, Ballanyi K, Jhamandas JH
    J. Biol. Chem., 2012-04-12;287(22):18820-30.
    Species: Human
    Sample Types: Cell Lysates
  85. Cross-Reactivity of Anthrax and C2 Toxin: Protective Antigen Promotes the Uptake of Botulinum C2I Toxin into Human Endothelial Cells.
    Authors: Kronhardt A, Rolando M, Beitzinger C, Stefani C, Leuber M, Flatau G, Popoff MR, Benz R, Lemichez E
    PLoS ONE, 2011-08-05;6(8):e23133.
    Species: Human
    Sample Types: Cell Lysates
  86. Glucagon-like peptide-1 (GLP-1) and the regulation of human invariant natural killer T cells: lessons from obesity, diabetes and psoriasis.
    Authors: Hogan AE, Tobin AM, Ahern T, Corrigan MA, Gaoatswe G, Jackson R, O'Reilly V, Lynch L, Doherty DG, Moynagh PN, Kirby B, O'Connell J, O'Shea D
    Diabetologia, 2011-07-09;54(11):2745-54.
    Species: Human
    Sample Types: Cell Lysates
  87. Hypoxia and prostaglandin e receptor 4 signalling pathways synergise to promote endometrial adenocarcinoma cell proliferation and tumour growth.
    Authors: Catalano RD, Wilson MR, Boddy SC
    PLoS ONE, 2011-05-12;6(5):e19209.
    Species: Human
    Sample Types: Cell Lysates
  88. gammadeltaT-cell function in sepsis is modulated by C5a receptor signalling.
    Authors: Han G, Geng S, Li Y, Chen G, Wang R, Li X, Ma Y, Shen B, Li Y
    Immunology, 2011-04-19;133(3):340-9.
    Species: Mouse
    Sample Types: Cell Lysates
  89. beta1-Adrenergic receptors activate two distinct signaling pathways in striatal neurons.
    Authors: Meitzen J, Luoma JI, Stern CM, Mermelstein PG
    J. Neurochem., 2011-01-19;116(6):984-95.
    Species: Rat
    Sample Types: Cell Lysates
  90. Anti-inflammatory role of microsomal prostaglandin E synthase-1 in a model of neuroinflammation.
    Authors: Brenneis C, Coste O, Altenrath K, Angioni C, Schmidt H, Schuh CD, Zhang DD, Henke M, Weigert A, Brune B, Rubin B, Nusing R, Scholich K, Geisslinger G
    J. Biol. Chem., 2010-11-12;286(3):2331-42.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  91. Mechanism of glucocorticoid protection of airway smooth muscle from proasthmatic effects of long-acting beta2-adrenoceptor agonist exposure.
    Authors: Nino G, Hu A, Grunstein JS, Grunstein MM
    J. Allergy Clin. Immunol., 2010-04-14;125(5):1020-7.
    Species: Human
    Sample Types: Cell Lysates
  92. Transforming growth factor beta1 inhibits cystic fibrosis transmembrane conductance regulator-dependent cAMP-stimulated alveolar epithelial fluid transport via a phosphatidylinositol 3-kinase-dependent mechanism.
    Authors: Roux J, Carles M, Koh H, Goolaerts A, Ganter MT, Chesebro BB, Howard M, Houseman BT, Finkbeiner W, Shokat KM, Paquet AC, Matthay MA, Pittet JF
    J. Biol. Chem., 2009-12-08;285(7):4278-90.
    Species: Rat
    Sample Types: Cell Lysates
  93. EP2 receptor mediated cAMP release is augmented by PGF 2 alpha activation of the FP receptor via the calcium-calmodulin pathway.
    Authors: Abera AB, Sales KJ, Catalano RD, Katz AA, Jabbour HN
    Cell. Signal., 2009-09-25;22(1):71-9.
    Species: Human
    Sample Types: Cell Lysates
  94. Mechanism regulating proasthmatic effects of prolonged homologous beta2-adrenergic receptor desensitization in airway smooth muscle.
    Authors: Nino G, Hu A, Grunstein JS, Grunstein MM
    Am. J. Physiol. Lung Cell Mol. Physiol., 2009-08-07;297(4):L746-57.
    Species: Human
    Sample Types: Cell Lysates
  95. Rational combined targeting of phosphodiesterase 4B and SYK in DLBCL.
    Authors: Kim SW, Rai D, McKeller MR, Aguiar RC
    Blood, 2009-04-15;113(24):6153-60.
    Species: Human
    Sample Types: Cell Lysates
  96. Reduced adiposity and high-fat diet-induced adipose inflammation in mice deficient for phosphodiesterase 4B.
    Authors: Zhang R, Maratos-Flier E, Flier JS
    Endocrinology, 2009-04-09;150(7):3076-82.
    Species: Mouse
    Sample Types: Cell Lysates
  97. Differential expression and functional characterization of luteinizing hormone receptor splice variants in human luteal cells: implications for luteolysis.
    Authors: Dickinson RE, Stewart AJ, Myers M, Millar RP, Duncan WC
    Endocrinology, 2009-02-26;150(6):2873-81.
    Species: Human
    Sample Types: Cell Lysates
  98. Exendin-4 has an anti-hypertensive effect in salt-sensitive mice model.
    Authors: Hirata K, Kume S, Araki S, Sakaguchi M, Chin-Kanasaki M, Isshiki K, Sugimoto T, Nishiyama A, Koya D, Haneda M, Kashiwagi A, Uzu T
    Biochem. Biophys. Res. Commun., 2009-01-14;380(1):44-9.
    Species: Mouse
    Sample Types: Urine
  99. Long term oncostatin M treatment induces an osteocyte-like differentiation on osteosarcoma and calvaria cells.
    Authors: Brounais B, David E, Chipoy C, Trichet V, Ferre V, Charrier C, Duplomb L, Berreur M, Redini F, Heymann D, Blanchard F
    Bone, 2009-01-03;44(5):830-9.
    Species: Mouse, Rat
    Sample Types: Cell Culture Supernates
  100. Human female hair follicles are a direct, nonclassical target for thyroid-stimulating hormone.
    Authors: Bodo E, Kromminga A, Bíró T, Borbiro I, Gáspár E, Zmijewski MA, van Beek N, Langbein L, Slominski AT, Paus R
    J. Invest. Dermatol., 2008-12-04;129(5):1126-39.
    Species: Human
    Sample Types: Cell Culture Supernates
  101. Inhibition of interleukin-1beta-induced matrix metalloproteinases 1 and 13 production in human osteoarthritic chondrocytes by prostaglandin D2.
    Authors: Zayed N, Afif H, Chabane N, Mfuna-Endam L, Benderdour M, Martel-Pelletier J, Pelletier JP, Motiani RK, Trebak M, Duval N, Fahmi H
    Arthritis Rheum., 2008-11-01;58(11):3530-40.
    Species: Human
    Sample Types: Cell Lysates
  102. Thiophenecarboxylate suppressor of cyclic nucleotides discovered in a small-molecule screen blocks toxin-induced intestinal fluid secretion.
    Authors: Tradtrantip L, Yangthara B, Padmawar P, Morrison C, Verkman AS
    Mol. Pharmacol., 2008-09-29;75(1):134-42.
    Species: Canine, Hamster, Rat
    Sample Types: Cell Lysates
  103. Dehydroepiandrosterone stimulates phosphorylation of FoxO1 in vascular endothelial cells via phosphatidylinositol 3-kinase- and protein kinase A-dependent signaling pathways to regulate ET-1 synthesis and secretion.
    Authors: Chen H, Lin AS, Li Y, Reiter CE, Ver MR, Quon MJ
    J. Biol. Chem., 2008-08-21;283(43):29228-38.
    Species: Bovine
    Sample Types: Cell Lysates
  104. Prostaglandin E2 stimulates Fas ligand expression via the EP1 receptor in colon cancer cells.
    Authors: O'Callaghan G, Kelly J, Shanahan F, Houston A
    Br. J. Cancer, 2008-07-22;99(3):502-12.
    Species: Human
    Sample Types: Cell Culture Supernates
  105. CT20126, a novel immunosuppressant, prevents collagen-induced arthritis through the down-regulation of inflammatory gene expression by inhibiting NF-kappaB activation.
    Authors: Lee SJ, Nam WD, Na HJ, Cho YL, Ha KS, Hwang JY, Lee H, Kim SO, Kwon YG, Kim YM
    Biochem. Pharmacol., 2008-04-18;76(1):79-90.
    Species: Human, Mouse
    Sample Types: Cell Culture Supernates
  106. Morphine withdrawal inhibits IL-12 induction in a macrophage cell line through a mechanism that involves cAMP.
    Authors: Kelschenbach J, Ninkovic J, Wang J, Krishnan A, Charboneau R, Barke RA, Roy S
    J. Immunol., 2008-03-15;180(6):3670-9.
    Species: Mouse
    Sample Types: Cell Lysates
  107. Acylated and unacylated ghrelin promote proliferation and inhibit apoptosis of pancreatic beta-cells and human islets: involvement of 3',5'-cyclic adenosine monophosphate/protein kinase A, extracellular signal-regulated kinase 1/2, and phosphatidyl inositol 3-Kinase/Akt signaling.
    Authors: Granata R, Settanni F, Biancone L, Trovato L, Nano R, Bertuzzi F, Destefanis S, Annunziata M, Martinetti M, Catapano F, Ghè C, Isgaard J, Papotti M, Ghigo E, Muccioli G
    Endocrinology, 2006-10-26;148(2):512-29.
    Species: Hamster
    Sample Types: Cell Lysates

FAQs

  1. The cAMP Parameter Assay Kit, Catalog # KGE002B, provides  a cell lysate preparation protocol. Can the cell freezing times be varied when using this protocol? 

    • Cell lysates were prepared using the lysate preparation protocol as described. While our scientists have not tested alternate preparation protocols, alteration of the freezing time for cells (including overnight freezing) and using more than two freeze-thaws is not expected to alter the assay results. To be safe, user can determine this with some preliminary testing.

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