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Canine IL-10 ELISA Kit - Quantikine

Catalog #: CA1000
13 Citations 2 Reviews Datasheet / COA / SDS
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CA1000
Canine IL-10 ELISA Standard Curve
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Product Details
Procedure
Citations (13)
FAQs
Supplemental Products
Reviews (2)
Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Canine IL-10 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity
3.8 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant canine IL-10
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Canine IL-10 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure canine IL-10 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant canine IL-10 and antibodies raised against the recombinant protein. Results obtained for naturally occurring canine IL-10 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural canine IL-10.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 53 144 533 59 152 512
Standard Deviation 2.9 7 20.1 5.1 9.3 21
CV% 5.5 4.9 3.8 8.6 6.1 4.1

Recovery

The recovery of canine IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=5) 93 75-104
EDTA Plasma (n=4) 89 83-93
Heparin Plasma (n=4) 87 83-93
Serum (n=5) 96 80-107

Linearity

To assess the linearity of the assay, samples containing or spiked with high concentrations of canine IL-10 in each matrix were diluted with Calibrator Diluent RD6-12 and assayed.
Canine IL-10 ELISA Linearity

Scientific Data

N/A Canine IL-10 ELISA Standard Curve View Larger

Canine IL-10 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-10

Interleukin-10 functions as an anti-inflammatory cytokine by inhibiting the expansion and activation of Th1 cells and Th17 cells and by promoting the development of M2 macrophages and regulatory T cells (Treg). Within a tumor microrenvironment, however, IL-10 can inhibit the expansion of both Treg and myeloid-derived suppressor cells (MDSC). IL-10 exerts protective effects including limiting tissue damage in arthritic inflammation and promoting muscle regeneration after injury, but it also contributes to the persistence of viral infections. IL-10 signals through a receptor complex composed of IL-10 R alpha and IL-10 R beta. IL-10 R beta additionally associates with IL-20 R alpha, IL-22 R alpha 1, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29.

Long Name:
Interleukin 10
Entrez Gene IDs:
3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 102133450 (Cynomolgus Monkey); 493683 (Feline); 100715618 (Guinea Pig); 2949786 (Viral)
Alternate Names:
CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or sample
  4.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
    6. 100 µL Conjugate
  6.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 5 times.
    8. 100 µL Substrate Solution
  8.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 100 µL Stop Solution
  9.   Add 100 µL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Canine IL-10 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Mycobacterium intracellulare induces a Th17 immune response via M1-like macrophage polarization in canine peripheral blood mononuclear cells
    Authors: S Kim, YS Hyun, HT Park, MK Shin, HS Yoo
    Scientific Reports, 2022-07-12;12(1):11818.
    Species: Canine
    Sample Types: Cell Culture Supernates
  2. Effect of Cannabidiol (CBD) on Canine Inflammatory Response: An Ex Vivo Study on LPS Stimulated Whole Blood
    Authors: E Gugliandol, P Licata, AF Peritore, R Siracusa, R D'Amico, M Cordaro, R Fusco, D Impellizze, R Di Paola, S Cuzzocrea, R Crupi, CD Interlandi
    Veterinary sciences, 2021-09-04;8(9):.
    Species: Canine
    Sample Types: Whole Blood
  3. Effect of modified ultrafiltration on cytokines and hemoconcentration in dogs undergoing cardiopulmonary bypass
    Authors: H Suzuki, N Oshima, T Watari
    J Vet Med Sci, 2020-10-05;0(0):.
    Species: Canine
    Sample Types: Plasma
  4. A preliminary study assessing cytokine production following ex vivo stimulation of whole blood with diet in dogs with chronic enteropathy
    Authors: A Kathrani, E Hall
    BMC Vet. Res., 2019-06-04;15(1):185.
    Species: Canine
    Sample Types: Cell Culture Supernates
  5. Clinical Assessment of Intravenous Endothelial Progenitor Cell Transplantation in Dogs
    Authors: SH Lee, JC Ra, HJ Oh, MJ Kim, EMN Setyawan, YB Choi, JW Yang, SK Kang, SH Han, GA Kim, BC Lee
    Cell Transplant, 2019-04-24;0(0):9636897188216.
    Species: Canine
    Sample Types: Serum
  6. Zoonotic intestinal helminths interact with the canine immune system by modulating T cell responses and preventing dendritic cell maturation
    Authors: J Junginger, K Raue, K Wolf, E Janecek, VM Stein, A Tipold, AR Günzel-Ape, C Strube, M Hewicker-T
    Sci Rep, 2017-09-04;7(1):10310.
    Species: Canine
    Sample Types: Cell Culture Supernates
  7. Higher neonatal growth rate and body condition score at 7�months are predictive factors of obesity in adult female Beagle dogs
    Authors: L Leclerc, C Thorin, J Flanagan, V Biourge, S Serisier, P Nguyen
    BMC Vet. Res., 2017-04-13;13(1):104.
    Species: Canine
    Sample Types: Plasma
  8. Immunometabolic parameters in overweight dogs during weight loss with or without an exercise program
    Authors: C R Bjørnvad
    Domest. Anim. Endocrinol., 2016-11-11;59(0):58-66.
    Species: Canine
    Sample Types: Plasma
  9. Use of a recombinant cysteine proteinase from Leishmania (Leishmania) infantum chagasi for the Immunotherapy of canine visceral Leishmaniasis.
    Authors: Ferreira J, Silva L, Longo-Maugeri I, Katz S, Barbieri C
    PLoS Negl Trop Dis, 2014-03-13;8(3):e2729.
    Species: Canine
    Sample Types: Serum
  10. Programmed death 1-mediated T cell exhaustion during visceral leishmaniasis impairs phagocyte function.
    Authors: Esch K, Juelsgaard R, Martinez P, Jones D, Petersen C
    J Immunol, 2013-10-23;191(11):5542-50.
    Species: Canine
    Sample Types: Cell Culture Supernates
  11. Clinical, parasitologic, and immunologic evolution in dogs experimentally infected with sand fly-derived Leishmania chagasi promastigotes.
    Authors: Travi BL, Osorio EY, Saldarriaga OA
    Am. J. Trop. Med. Hyg., 2009-12-01;81(6):994-1003.
    Species: Canine
    Sample Types: Cell Culture Supernates
  12. A functional comparison of canine and murine bone marrow derived cultured mast cells.
    Authors: Lin TY, London CA
    Vet. Immunol. Immunopathol., 2006-10-06;114(3):320-34.
    Species: Canine
    Sample Types: Cell Culture Supernates
  13. Measurement of serum interleukin-10 in the dog.
    Authors: Kjelgaard-Hansen M, Luntang-Jensen M, Willesen J, Jensen AL
    Vet. J., 2006-02-03;173(2):361-5.
    Species: Canine
    Sample Types: Serum

FAQs

  1. Assay Diluent RD1-14 contains visible precipitate. Is this acceptable to use?

    • A small amount of precipitate is expected and acceptable in Assay Diluent RD1-14. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.

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Reviews for Canine IL-10 Quantikine ELISA Kit

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Canine IL-10 Quantikine ELISA Kit
By Anonymous on 05/26/2020
Sample Tested: Cell Culture Media

This immune assay is clear, consistent, and reliable. Would suggest this product to colleagues. Good assay.

Canine IL-10 Quantikine ELISA Kit
By Anonymous on 12/01/2017
Sample Tested: canine serum samples,Serum and Plasma