Canine IL-18/IL-1F4 Antibody

Catalog #: MAB2924 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2924
MAB2924-SP

All IL-18/IL-1F4 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Canine IL-18/IL-1F4 Antibody Summary

Species Reactivity

Canine

Specificity

Detects canine IL‑18/IL‑1F4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) IL-18, recombinant mouse (rm) IL-18, recombinant porcine IL-18, recombinant rat IL-18, recombinant rhesus macaque IL-18, rhIL‑36Ra, rhIL-1F7, rhIL-36 gamma, rhIL-1F10, rmIL-1ra, rmIL-36 gamma, or rmIL-36 alpha is observed.

Source

Monoclonal Mouse IgG₁ Clone # 314820

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant canine IL‑18/IL‑1F4
Tyr37-Ser193
Accession # Q9XSR0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Canine IL‑18/IL‑1F4

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-18/IL-1F4

Interleukin 18, also known as interferon-gamma-inducing factor (IGIF) or IL-1 gamma, is an 18 kDa member of the IL-1 superfamily which shares biologic activities with IL‑12. IL-18 is expressed by liver cells, monocyte/macrophages, osteoblasts and keratinocytes. Mature canine IL-18 shares 73% sequence identity with human IL-18. Caspase-1 (IL-1 beta-converting enzyme) has been shown to process pro-IL-18 to active IL-18. Human IL-18 has been shown to enhance NK cell activity, induce the production of IFN-gamma and GM-CSF and inhibit the production of IL-10 by PBMCs.

Long Name

Interleukin 18

Entrez Gene IDs

3606 (Human); 16173 (Mouse); 29197 (Rat); 397057 (Porcine); 574151 (Primate)

Alternate Names

Iboctadekin; IFN-gamma-inducing factor; IGIF; IGIFIL-1 gamma; IL18; IL-18; IL-18MGC12320; IL-1F4; IL1F4iboctadekin; IL-1g; Interferon gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor); Interleukin-1 gamma; interleukin-18