CellXVivo Human Th2 Cell Differentiation Kit

For the differentiation of Th2 cells from a preparation of naïve CD4⁺ T cells.

Why Differentiate Th2 Cells In Vitro?

T helper type 2 (Th2) cells are a lineage of CD4⁺ effector T cells that provide host protection against intestinal helminths and extracellular bacteria in addition to support for B cell-dependent humoral responses. Pathological Th2 cell activity is a hallmark of allergic inflammation and asthma. Differentiation of CD4⁺ effector cells into the Th2 lineage is promoted by cytokines such as IL-4 in combination with either IL-2, IL-7, or TSLP. Th2 cells secrete IL-4, IL-5, IL-9, IL-13, and IL17E/IL-25. The CellXVivo™ Human Th2 Cell Differentiation Kit contains all necessary components to differentiate human naïve CD4⁺ T cells into Th2 polarized cells.

This kit contains the following optimized proteins and reagents to drive efficient differentiation of naïve CD4⁺ T cells into Th2 polarized cells.

• Mouse Anti-Human CD3 Antibody
• Human Th2 Differentiation Supplements
• Reconstitution Buffers
• Wash Buffer (20X)

Provides sufficient reagents for the differentiation of one 24-well plate or 125 tests in a 96-well format.

Stability and Storage

Store the unopened kit at 2 to 8°C. After opening the kit, Mouse Anti-Human CD3 Antibody and Human Th2 Reagents 1-4 may be stored at 2-8°C under sterile conditions for up to 30 days or at -20°C to -70°C in a manual defrost freezer for up to 3 months. Reconstitution Buffer 1, Reconstitution Buffer 2, and 20X Wash Buffer may be stored under sterile conditions for up to 12 months at 2 to 8°C. Do not use beyond the expiration date of the kit.