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CometAssay Electrophoresis System II

Catalog #: 4250-050-ES
19 Citations Datasheet / COA / SDS
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4250-050-ES
Product Details
Citations (19)
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Summary Product Datasheets

CometAssay Electrophoresis System II Summary

Enables optimization and standardization of alkaline and neutral comet assays.
 

Key Benefits

• Maintains constant buffer temperature with cooling pack chamber and one-piece molded plastic body.
• Maintains optimal buffer level for consistent results with overlay.
• Specially designed trays accommodate 2, 20 and 96 well slides and maintain correct position during electrophoresis.
• Optimized for use with CometAssay Kits and CometAssay Control Cells.

What is the CometAssay?

The Comet assay, or single cell gel electrophoresis assay, provides a simple and effective method for evaluating DNA damage in cells. The principle of the assay is based upon the ability of denatured, cleaved DNA fragments to migrate out of the nucleoid under the influence of an electric field, whereas undamaged DNA migrates slower and remains within the confines of the nucleoid when a current is applied. Evaluation of the DNA “comet” tail shape and migration pattern allows for assessment of DNA damage. The general Comet assay protocol includes first immobilizing cells in a bed of low melting point agarose, gently lysing the cells, treating cells with neutral or alkali solutions to unwind and denature the DNA as well as hydrolyze sites of damage. DNA is then subjected to electrophoresis and stained with a fluorescent DNA intercalating dye.

Why Use the CometAssay Electrophoresis System?

CometAssay ES Il enables investigators to consistently optimize alkaline and neutral comet assays for highly reproducible results, and to standardize electrophoresis methods between individual users and laboratories. Comet assay results can be variable depending on temperature, distance between electrodes, and buffer height. This specialized electrophoresis unit helps resolve these variables.

Specifications

Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended on the label.
Storage
Store the unopened product at room temperature. Do not use past expiration date.
Species
N/A

Limitations

For research use only. Not for diagnostic use.

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Citations for CometAssay Electrophoresis System II

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. PRMT1 inhibition perturbs RNA metabolism and induces DNA damage in clear cell renal cell carcinoma
    Authors: Walton, J;Ng, ASN;Arevalo, K;Apostoli, A;Meens, J;Karamboulas, C;St-Germain, J;Prinos, P;Dmytryshyn, J;Chen, E;Arrowsmith, CH;Raught, B;Ailles, L;
    Nature communications  2024-09-19
  2. MET Inhibitor Capmatinib Radiosensitizes MET Exon 14-Mutated and MET-Amplified Non-Small Cell Lung Cancer
    Authors: Ramesh, S;Cifci, A;Javeri, S;Minne, R;Longhurst, CA;Nickel, KP;Kimple, RJ;Baschnagel, AM;
    bioRxiv : the preprint server for biology  2023-10-27
  3. Restoring bone marrow niche function rejuvenates aged hematopoietic stem cells by reactivating the DNA Damage Response
    Authors: P Ramalingam, MC Gutkin, MG Poulos, T Tillery, C Doughty, A Winiarski, AG Freire, S Rafii, D Redmond, JM Butler
    Nature Communications, 2023-04-10;14(1):2018.  2023-04-10
  4. The N6-methyladenosine methyltransferase METTL16 enables erythropoiesis through safeguarding genome integrity
    Authors: M Yoshinaga, K Han, DW Morgens, T Horii, R Kobayashi, T Tsuruyama, F Hia, S Yasukura, A Kajiya, T Cai, PHC Cruz, A Vandenbon, Y Suzuki, Y Kawahara, I Hatada, MC Bassik, O Takeuchi
    Nature Communications, 2022-10-28;13(1):6435.  2022-10-28
  5. Discovery and Validation of Potential Serum Biomarkers with Pro-Inflammatory and DNA Damage Activities in Ulcerative Colitis: A Comprehensive Untargeted Metabolomic Study
    Authors: M Li, R Zhang, M Xin, Y Xu, S Liu, B Yu, B Zhang, J Liu
    Metabolites, 2022-10-20;12(10):.  2022-10-20
  6. MED1, a novel binding partner of BRCA1, regulates homologous recombination and R-loop processing
    Authors: H Honjoh, M Tanikawa, O Wada-Hirai, K Oda, H Inaba, A Kukita, Y Kawata, M Kusakabe, S Tsuchimoch, A Taguchi, Y Miyamoto, K Sone, T Tsuruga, M Mori-Uchin, Y Matsumoto, Y Osuga
    Scientific Reports, 2022-10-13;12(1):17140.  2022-10-13
  7. A multi-functional role for the MCM8/9 helicase complex in maintaining fork integrity during replication stress
    Authors: WC Griffin, DR McKinzey, KN Klinzing, R Baratam, A Eliyapura, MA Trakselis
    Nature Communications, 2022-08-30;13(1):5090.  2022-08-30
  8. Genetic instability from a single S phase after whole-genome duplication
    Authors: S Gemble, R Wardenaar, K Keuper, N Srivastava, M Nano, AS Macé, AE Tijhuis, SV Bernhard, DCJ Spierings, A Simon, O Goundiam, H Hochegger, M Piel, F Foijer, Z Storchová, R Basto
    Nature, 2022-03-30;604(7904):146-151.  2022-03-30
  9. CHK1 phosphorylates PRIMPOL to promote replication stress tolerance
    Authors: KPM Mehta, V Thada, R Zhao, A Krishnamoo, M Leser, K Lindsey Ro, D Cortez
    Science Advances, 2022-03-30;8(13):eabm0314.  2022-03-30
  10. SLFN11 is Widely Expressed in Pediatric Sarcoma and Induces Variable Sensitization to Replicative Stress Caused By DNA-Damaging Agents
    Authors: J Gartrell, M Mellado-La, MR Clay, A Bahrami, NA Sahr, A Sykes, K Blankenshi, L Hoffmann, J Xie, HP Cho, N Twarog, M Connelly, KK Yan, J Yu, SN Porter, SM Pruett-Mil, G Neale, CL Tinkle, SM Federico, EA Stewart, AA Shelat
    Molecular Cancer Therapeutics, 2021-08-19;20(11):2151-2165.  2021-08-19
  11. The long-noncoding RNA SOCS2-AS1 suppresses endometrial cancer progression by regulating AURKA degradation
    Authors: F Jian, X Che, J Zhang, C Liu, G Liu, Y Tang, W Feng
    Cell Death & Disease, 2021-04-06;12(4):351.  2021-04-06
  12. Topoisomerase 2&beta Induces DNA Breaks To Regulate Human Papillomavirus Replication
    Authors: P Kaminski, S Hong, T Kono, P Hoover, L Laimins
    MBio, 2021-02-09;12(1):.  2021-02-09
  13. Aldehyde dehydrogenase inhibitors promote DNA damage in ovarian cancer and synergize with ATM/ATR inhibitors
    Authors: E Grimley, AJ Cole, TT Luong, SC McGonigal, S Sinno, D Yang, KA Bernstein, RJ Buckanovic
    Theranostics, 2021-01-20;11(8):3540-3551.  2021-01-20
  14. Photomutagenicity of chlorpromazine and its N-demethylated metabolites assessed by NGS
    Authors: JAG Agúndez, E García-Mar, G García-Lai, MA Miranda, I Andreu
    Sci Rep, 2020-04-23;10(1):6879.  2020-04-23
  15. The human Shu complex functions with PDS5B and SPIDR to promote homologous recombination
    Authors: J Martino, GJ Brunette, J Barroso-Go, TN Moiseeva, CM Smith, CJ Bakkenist, RJ O'Sullivan, KA Bernstein
    Nucleic Acids Res., 2019-11-04;47(19):10151-10165.  2019-11-04
  16. TRIM66 reads unmodified H3R2K4 and H3K56ac to respond to DNA damage in embryonic stem cells
    Authors: J Chen, Z Wang, X Guo, F Li, Q Wei, X Chen, D Gong, Y Xu, W Chen, Y Liu, J Kang, Y Shi
    Nat Commun, 2019-09-19;10(1):4273.  2019-09-19
  17. Hypoosmotic stress induces R loop formation in nucleoli and ATR/ATM-dependent silencing of nucleolar transcription
    Authors: AK Velichko, NV Petrova, AV Luzhin, OS Strelkova, N Ovsyanniko, II Kireev, NV Petrova, SV Razin, OL Kantidze
    Nucleic Acids Res., 2019-07-26;0(0):.  2019-07-26
  18. Androgen receptor signaling regulates DNA repair in prostate cancers.
    Authors: Polkinghorn W, Parker J, Lee M, Kass E, Spratt D, Iaquinta P, Arora V, Yen W, Cai L, Zheng D, Carver B, Chen Y, Watson P, Shah N, Fujisawa S, Goglia A, Gopalan A, Hieronymus H, Wongvipat J, Scardino P, Zelefsky M, Jasin M, Chaudhuri J, Powell S, Sawyers C
    Cancer Discov, 2013-09-11;3(11):1245-53.  2013-09-11
  19. FBH1 promotes DNA double-strand breakage and apoptosis in response to DNA replication stress.
    Authors: Jeong Y, Rossi M, Cermak L, Saraf A, Florens L, Washburn M, Sung P, Schildkraut C, Pagano M
    J Cell Biol, 2013-01-14;200(2):141-9.  2013-01-14

FAQs

  1. Will any commercial horizontal gel electrophoresis device work for the Comet Assay?

    • Electrophoresis conditions described for FLARE™ Assay, CometAssay® and CometChip® Kits were optimized for the CometAssay® Electrophoresis System (Catalog # 4250-050-ES). Conventional slab gel electrophoresis chambers are not designed to eliminate known causes of comet assay variability (alkaline pH, buffer height, temperature, slide orientation). They can be used, but they require optimization by the user to achieve consistent results.

  2. What power supply is recommended for the CometAssay Electrophoresis System?

    • We recommend PowerPac™ HC High-Current Power Supply (1645052), available from Bio-Rad.

  3. Which adaptor can be used with the PowerPac ( Bio-Rad®, Catalog # 1645052) recommended for the CometAsay Electrophoresis System II?

    • The PowerPac Adator (Catalog # 1645064) can be used with the PowerPac recommended for the CometAsay Electrophoresis System II.

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