Cotton Rat CCL2/JE/MCP-1 Antibody

Catalog #: MAB2137 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2137
MAB2137-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Cotton Rat CCL2/JE/MCP-1 Antibody Summary

Species Reactivity

Cotton Rat

Specificity

Detects cotton rat CCL2/JE/MCP‑1 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse CCL2 is observed and no cross-reactivity with recombinant human CCL1, 2, 3, 5, 7, 8, 11, 13, 14, 15, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, recombinant mouse CCL1, 3, 4, 5, 6, 7, 9, 11, 12, 17, 19, 20, 21, 22, 24, 25, or recombinant rat CCL20 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 238607

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant cotton rat CCL2/JE/MCP‑1
Gln25-Asn150
Accession # AAN85636

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Cotton Rat CCL2/JE/MCP‑1

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL2/JE/MCP-1

CCL2, also known as JE or MCP-1 (monocyte chemoattractant protein-1), is a CC chemokine produced by fibroblasts, macrophages, astrocytes, mast cells, endothelial cells and osteoblasts. It functions as a chemoattractant through ligations with CCR2 on monocytes, macrophages and lymphocytes.

Entrez Gene IDs

6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)

Alternate Names

C-C motif chemokine ligand 2; CCL2; GDCF-2; HC11; HSMCR30; MCAF; Mcp1; MCP-1; SCYA2; SMC-CF