Cotton Rat CCL3/MIP-1 alpha Antibody

Catalog #: MAB1061
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1061
MAB1061-SP

Chemotaxis Induced by CCL3/MIP‑1 alpha and Neutralization by Cotton Rat CCL3/MIP‑1 alpha Antibody.

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Cotton Rat CCL3/MIP-1 alpha Antibody Summary

Species Reactivity

Cotton Rat

Specificity

Detects cotton rat CCL3/MIP-1 alpha in direct ELISAs. In direct ELISAs, this antibody shows approximately 50% cross-reactivity with recombinant human CCL3/MIP‑1 alpha and recombinant cotton rat CCL4/MIP-1 beta and no cross-reactivity with recombinant cotton rat CCL5, recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, recombinant mouse CCL1, 2, 3, 4, 5, 6, 7, 8, 9/10/MIP-1 gamma, 11, 12, 17, 19, 20, 21, 22, 24, 25, 27, 28, or recombinant rat CCL20.

Source

Monoclonal Mouse IgG_2A Clone # 175024

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant cotton rat CCL3/MIP-1 alpha
Ala24-Ala92
Accession # AAL26704

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Cotton Rat CCL3/MIP‑1 alpha (Catalog # 1061-RM)

Neutralization

Measured by its ability to neutralize CCL3/MIP‑1 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND₅₀) is typically 0.05-0.3 µg/mL in the presence of 10 ng/mL Recombinant Cotton Rat CCL3/MIP‑1 alpha.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Chemotaxis Induced by CCL3/MIP‑1 alpha and Neutralization by Cotton Rat CCL3/MIP‑1 alpha Antibody. Recombinant Cotton Rat CCL3/MIP-1a (Catalog # 1061-RM) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Cotton Rat CCL3/MIP-1a (10 ng/mL) is neutralized (green line) by increasing concentrations of Cotton Rat CCL3/MIP-1a Monoclonal Antibody (Catalog # MAB1061). The ND₅₀ is typically 0.05-0.3 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL3/MIP-1 alpha

MIP-1 alpha is a beta family (CC) chemokine and has been designated CCL3. MIP-1 alpha and CCL4/MIP-1 beta, two closely related but distinct proteins, were originally purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Cotton rat CCL3 cDNA encodes a 92 amino acid (aa) precursor protein with a 23 aa putative signal peptide. Mature cotton rat CCL3 shares approximately 70% amino acid identity with human CCL3. CCL3 is expressed in a wide variety of cells, including lymphocytes, fibroblasts, epithelial cells, and monocytes/macrophages. CCL3 has been shown to play an important role in the recruitment of mononuclear cells. Additionally, CCL3 has been reported to have chemoattractant and adhesive effects on lymphocytes, preferentially promoting the chemotaxis of Th1 cells. CCL3 has also been shown to attract B cells, eosinophils, and dendritic cells. In addition, CCL3 augments cytolytic activity of NK cells (1). CCL3 has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. It has been demonstrated that CCL3 can bind the chemokine receptors CCR1 and CCR5 (2).

References

Robertson, M. (2002) J. Leukoc. Biol. 71:173.
Zlotnik, A. et al. (2000) Immunity 12:121.

Entrez Gene IDs

6348 (Human); 20302 (Mouse); 25542 (Rat); 448787 (Canine); 102136134 (Cynomolgus Monkey)

Alternate Names

C-C motif chemokine 3; MIP1-(a); AI323804; CCL3; chemokine (C-C motif) ligand 3; G0S19-1; LD78a; LD78alpha; MIP1 alpha; MIP-1 alpha; MIP1A; MIP-1alpha; MIP1-alpha; PAT 464.1; SCYA3; SIS-beta

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Citation for Cotton Rat CCL3/MIP-1 alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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