Cotton Rat IFN-gamma Antibody

Catalog #: AF565
6 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF565
AF565-SP

IFN‑ gamma Inhibition of EMC-induced Cytopathy and Neutralization by Cotton Rat IFN‑ gamma Antibody.

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All IFN-gamma Antibodies

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Citations (6)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Cotton Rat IFN-gamma Antibody Summary

Species Reactivity

Cotton Rat

Specificity

Detects cotton rat IFN‑ gamma in ELISAs and Western blots. In sandwich immunoassays, less than 0.25% cross-reactivity with recombinant canine IFN-gamma, recombinant feline IFN-gamma, and recombinant human IFN-gamma, recombinant mouse IFN-gamma, recombinant rat IFN-gamma, and recombinant porcine IFN-gamma is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant cotton rat IFN-gamma
His21-Ile170
Accession # Q9QXX2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Cotton Rat IFN-gamma (Catalog # 565-RG)

Cotton Rat IFN-gamma Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

0.2-0.8 µg/mL

Use in combination with:

Detection Reagent: Cotton Rat IFN‑ gamma Biotinylated Antibody (Catalog # BAF565)

Standard: Recombinant Cotton Rat IFN-gamma Protein (Catalog # 565-RG)

Neutralization

Measured by its ability to neutralize IFN‑ gamma inhibition of EMC-induced cytopathy in the LCRT cotton rat fibrosarcoma cell line. The Neutralization Dose (ND₅₀) is typically 0.3-1.2 µg/mL in the presence of 20 ng/mL Recombinant Cotton Rat IFN‑ gamma.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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IFN‑ gamma Inhibition of EMC-induced Cytopathy and Neutralization by Cotton Rat IFN‑ gamma Antibody. Recombinant Cotton Rat IFN-gamma (Catalog # 565-RG) reduces the encephalomyocarditis (EMC)-induced cytopathy in the LCRT cotton rat fibrosarcoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMC activity elicited by Recombinant Cotton Rat IFN-gamma (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF565). The ND₅₀ is typically 0.3-1.2 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-gamma

Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature cotton rat IFN-gamma exists as a noncovalently linked homodimer of 20‑25 kDa variably glycosylated subunits (3). It shares 43%‑50% amino acid sequence identity with bovine, canine, equine, feline, human, mouse, rat, rhesus, and porcine IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).

References

Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
Pestka, S. et al. (2004) Immunol. Rev. 202:8.
Houard, S. et al. (1999) Gene 240:107.
Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401.
Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.

Long Name

Interferon gamma

Entrez Gene IDs

3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)

Alternate Names

IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Cotton Rat IFN-gamma Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Immunization with Live Human Rhinovirus (HRV) 16 Induces Protection in Cotton Rats against HRV14 Infection
Authors: MC Patel, LM Pletneva, MS Boukhvalov, SN Vogel, AE Kajon, JCG Blanco
Front Microbiol, 2017-08-31;8(0):1646.
Species: Cotton Rat
Sample Types: Whole Cells
Applications: ELISA Development (Capture)
Development of Acquired Immunity following Repeated Respiratory Syncytial Virus Infections in Cotton Rats
PLoS ONE, 2016-05-25;11(5):e0155777.
Species: Cotton Rat
Sample Types: Protein
Applications: Flow Cytometry
Role of AKT kinase in measles virus replication.
Authors: Carsillo M, Kim D, Niewiesk S
J. Virol., 2009-11-25;84(4):2180-3.
Species: Cotton Rat
Sample Types: Whole Cells
Applications: ELISpot Development
Sendai virus recombinant vaccine expressing hPIV-3 HN or F elicits protective immunity and combines with a second recombinant to prevent hPIV-1, hPIV-3 and RSV infections.
Authors: Zhan X, Slobod KS, Krishnamurthy S, Luque LE, Takimoto T, Jones B, Surman S, Russell CJ, Portner A, Hurwitz JL
Vaccine, 2008-05-01;26(27):3480-8.
Species: Cotton Rat
Sample Types: Peptide
Applications: ELISpot Development
Respiratory syncytial virus (RSV) fusion protein expressed by recombinant Sendai virus elicits B-cell and T-cell responses in cotton rats and confers protection against RSV subtypes A and B.
Authors: Zhan X, Hurwitz JL, Krishnamurthy S, Takimoto T, Boyd K, Scroggs RA, Surman S, Portner A, Slobod KS
Vaccine, 2007-11-05;25(52):8782-93.
Species: Cotton Rat
Sample Types: Cell Culture Supernates
Applications: ELISA Development
Developmental pattern of expression of BMP receptors and Smads and activation of Smad1 and Smad5 by BMP9 in mouse basal forebrain.
Authors: Lopez-Coviella I, Mellott TM, Kovacheva VP, Berse B, Slack BE, Zemelko V, Schnitzler A, Blusztajn JK
Brain Res., 2006-04-13;1088(1):49-56.
Species: Mouse
Sample Types: Cell Lysates
Applications: Immunoprecipitation