Cotton Rat IL-1 alpha /IL-1F1 Antibody

Catalog #: MAB552 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB552
MAB552-SP

Cell Proliferation Induced by IL‑1 alpha /IL‑1F1 and Neutral-ization by Cotton Rat IL‑1 alpha / IL‑1F1 Antibody.

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Cotton Rat IL-1 alpha /IL-1F1 Antibody Summary

Species Reactivity

Cotton Rat

Specificity

Detects cotton rat IL‑1 alpha /IL‑1F1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant rat (rr) IL-1 alpha is observed and 20% cross-reactivity with recombinant mouse (rm) IL-1 alpha is observed. In Western blots, no cross-reactivity with recombinant human IL-1 alpha, rmIL-1 alpha, recombinant porcine IL-1 alpha, rrIL-1 beta, and rrIL-18 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 140221

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant cotton rat IL‑1 alpha /IL‑1F1
Ser115-Pro269
Accession # AAK94011

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Cotton Rat IL‑1 alpha /IL‑1F1 (Catalog # 552-R1)

Neutralization

Measured by its ability to neutralize IL‑1 alpha /IL‑1F1-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J. A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND₅₀) is typically 1-5 µg/mL in the presence of 50 pg/mL Recombinant Cotton Rat IL‑1 alpha /IL‑1F1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by IL‑1 alpha /IL‑1F1 and Neutral-ization by Cotton Rat IL‑1 alpha / IL‑1F1 Antibody. Recombinant Cotton Rat IL-1a/IL-1F1 (Catalog # 552-R1) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Cotton Rat IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Cotton Rat IL-1a/IL-1F1 Monoclonal Antibody (Catalog # MAB552). The ND₅₀ is typically 1-5 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 alpha/IL-1F1

Interleukin 1 (IL-1) is a name that designates two proteins, IL-1 alpha and IL-1 beta, that are the products of distinct genes, but show approximately 25% amino acid sequence identity and recognize the same cell surface receptors. Although IL-1 production is generally considered to be a consequence of inflammation, evidence suggests that IL-1 is also temporarily up‑regulated during bone formation and the menstrual cycle and can be induced in response to nervous system stimulation. In response to stimuli produced by inflammatory agents, infections, or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cells is seen. Cells in particular known to produce IL-1 include osteoblasts, monocytes, macrophages, keratinocytes, Kupffer cells, hepatocytes, thymic and salivary gland epithelium, Schwann cells, fibroblasts and glia (oligodendroglia, astrocytes and microglia).

IL-1 alpha and IL-1 beta are both synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17,000 Daltons. Neither precursor contains a typical hydrophobic signal peptide sequence and most of the precursor form of IL‑1 alpha remains in the cytosol of cells, although there is evidence for a membrane-bound form of the precursor form of IL-1 alpha. The IL-1 alpha precursor reportedly shows full biological activity in the EL-4 assay. Among various species, the amino acid sequence of mature IL-1 alpha is conserved 60% to 70% and human, porcine, rat and cotton rat IL-1 has been found to be biologically active on murine cell lines. Both forms of IL-1 bind to the same receptors, designated as type I and type II. Evidence suggests that only the type I receptor is capable of signal transduction and that the type II receptor may function as a decoy, binding IL-1 and thus preventing the binding of IL-1 to the type I receptor.

References

Dower, S.K. and J.E. Sims (1994) Guidebook to Cytokines and their receptors, Nicole, N.A. (ed), Oxford University Press, New York, p. 17.

Long Name

Interleukin 1 alpha

Entrez Gene IDs

3552 (Human); 16175 (Mouse); 24493 (Rat); 397094 (Porcine); 101926546 (Cynomolgus Monkey)

Alternate Names

BAF; Hematopoietin-1; IL1 alpha; IL-1 alpha; IL1; IL1A; IL-1A; IL1-ALPHA; IL1F1; IL-1F1; IL1F1hematopoietin-1; interleukin 1, alpha; interleukin-1 alpha; LAF; LEM; preinterleukin 1 alpha; pro-interleukin-1-alpha