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CRISPR-Cas13a Antibody

Catalog #: MAB10462 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB10462-100
MAB10462-SP
Detection of CRISPR-Cas13a by Western Blot.
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CRISPR-Cas13a Antibody Summary

Specificity
Detects CRISPR-Cas13a in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 1020221
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant Leptotrichia wadei Cas13a
Lys2-Glu1152
Accession # WP_021746774
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
Recombinant Leptotrichia wadei Cas13a protein

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot View Larger

Detection of CRISPR-Cas13a by Western Blot. Western blot shows recombinant Leptotrichia wadei Cas 13a protein. PVDF membrane was probed with 2 µg/mL of Mouse Anti-CRISPR-Cas13a Monoclonal Antibody (Catalog # MAB10462) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for CRISPR-Cas13a at approximately 143 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CRISPR-Cas13a

Long Name
CRISPR-associated Endoribonuclease 13a
Alternate Names
CRISPR-Cas13a

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