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Cultrex 3-D Culture Matrix Laminin I

Catalog #: 3446-005-01
13 Citations Datasheet / COA / SDS
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3446-005-01
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Citations (13)
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Cultrex 3-D Culture Matrix Laminin I Summary

Cultrex 3-D Culture Matrix Laminin I is an extracellular matrix hydrogel that directs cells to grow in three dimensions and assemble into organotypic structures in vitro.

Why Use Cultrex 3-D Culture Matrix Laminin I?

Cultrex 3-D Culture Matrix Laminin I is purified from Engelbreth-Holm-Swarm (EHS) tumor and is provided at a high concentration that is capable of polymerizing to form a hydrogel at 37°C. Laminin I is a major component of the basement membrane which is a continuous sheet of specialized extracellular matrix that forms an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that plays an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. Cultrex 3-D Culture Matrix Laminin I is a purified basement membrane protein that has been developed, produced and qualified specifically for use in 3-D culture studies. Cultrex 3-D Culture Matrix Laminin I may also be used as to supplement customized hydrogel or medium formulations for cell culture.

Negative for the presence of bacteria and fungi.

Specifications

Source
Murine Engelbreth-Holm-Swarm (EHS) tumor
Sterility Testing
No bacterial or fungal growth detected following 14 days in culture
Testing Cell Culture
3-D Culture - Laminin I Supports differentiation of a human epithelial cell line derived from mammary gland (MCF-10A) into acinar structures.

Cell Attachment - Tested for the ability to Supports cell attachment and spreading of MG63 human osteosarcoma cells.
Stability
Product is stable for a minimum of 3 months from date of shipment. See lot specific Certificate of Analysis for expiration date.
Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store at ≤ -20 °C in a manual defrost freezer. For optimal stability, store at ≤ -70 °C. Avoid freeze-thaw cycles
Species
Mouse

Limitations

For research use only. Not for diagnostic use.

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Citations for Cultrex 3-D Culture Matrix Laminin I

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Altered mRNA transport and local translation in iNeurons with RNA binding protein knockdown
    Authors: Dargan, R;Mikheenko, A;Johnson, NL;Packer, B;Li, Z;Craig, EJ;Sarbanes, SL;Bereda, C;Mehta, PR;Keuss, M;Nalls, MA;Qi, YA;Weller, CA;Fratta, P;Ryan, VH;
    bioRxiv : the preprint server for biology  2024-09-27
  2. N-cadherin dynamically regulates pediatric glioma cell migration in complex environments
    Authors: Kim, D;Olson, JM;Cooper, JA;
    bioRxiv : the preprint server for biology  2024-01-11
  3. Endolysosomal TRPML1 channel regulates cancer cell migration by altering intracellular trafficking of E-cadherin and ?1-integrin
    Authors: Frey, N;Ouologuem, L;Blenninger, J;Siow, WX;Thorn-Seshold, J;Stöckl, J;Abrahamian, C;Fröhlich, T;Vollmar, AM;Grimm, C;Bartel, K;
    The Journal of biological chemistry  2023-12-21
  4. Hierarchical assembly of tryptophan zipper peptides into stress-relaxing bioactive hydrogels
    Authors: Nguyen, AK;Molley, TG;Kardia, E;Ganda, S;Chakraborty, S;Wong, SL;Ruan, J;Yee, BE;Mata, J;Vijayan, A;Kumar, N;Tilley, RD;Waters, SA;Kilian, KA;
    Nature communications  2023-10-23
  5. Combinatorial selective ER-phagy remodels the ER during neurogenesis
    Authors: Hoyer, MJ;Smith, IR;Paoli, JC;Jiang, Y;Paulo, JA;Harper, JW;
    bioRxiv : the preprint server for biology  2023-06-26
  6. Elevated FOXG1 in glioblastoma stem cells cooperates with Wnt/?-catenin to induce exit from quiescence
    Authors: Robertson, FL;O'Duibhir, E;Gangoso, E;Bressan, RB;Bulstrode, H;Marqu�s-Torrej�n, M�;Ferguson, KM;Blin, C;Grant, V;Alfazema, N;Morrison, GM;Pollard, SM;
    Cell reports  2023-05-26
  7. Embryonic Stem Cells Can Generate Oral Epithelia under Matrix Instruction
    Authors: Das, R;Harper, L;Kitajima, K;Osman, TA;Cimpan, MR;Johannssen, AC;Suliman, S;Mackenzie, IC;Costea, DE;
    International journal of molecular sciences  2023-04-22
  8. A reference human induced pluripotent stem cell line for large-scale collaborative studies
    Authors: CB Pantazis, A Yang, E Lara, JA McDonough, C Blauwendra, L Peng, H Oguro, J Kanaujiya, J Zou, D Sebesta, G Pratt, E Cross, J Blockwick, P Buxton, L Kinner-Bib, C Medura, C Tompkins, S Hughes, M Santiana, F Faghri, MA Nalls, D Vitale, S Ballard, YA Qi, DM Ramos, KM Anderson, J Stadler, P Narayan, J Papademetr, L Reilly, MP Nelson, S Aggarwal, LU Rosen, P Kirwan, V Pisupati, SL Coon, SW Scholz, T Priebe, M Öttl, J Dong, M Meijer, LJM Janssen, VS Lourenco, R van der Ka, D Crusius, D Paquet, AC Raulin, G Bu, A Held, BJ Wainger, RMC Gabriele, JM Casey, S Wray, D Abu-Bonsra, CL Parish, MS Beccari, DW Cleveland, E Li, IVL Rose, M Kampmann, C Calatayud, P Verstreken, L Heinrich, MY Chen, B Schüle, D Dou, ELF Holzbaur, MC Zanellati, R Basundra, M Deshmukh, S Cohen, R Khanna, M Raman, ZS Nevin, M Matia, J Van Lent, V Timmerman, BR Conklin, K Johnson Ch, K Zhang, S Funes, DA Bosco, L Erlebach, M Welzer, D Kronenberg, G Lyu, E Arenas, E Coccia, L Sarrafha, T Ahfeldt, JC Marioni, WC Skarnes, MR Cookson, ME Ward, FT Merkle
    Cell Stem Cell, 2022-12-01;29(12):1685-1702.e22.  2022-12-01
  9. Quantification of spatial subclonal interactions enhancing the invasive phenotype of pediatric glioma
    Authors: H Tari, K Kessler, N Trahearn, B Werner, M Vinci, C Jones, A Sottoriva
    Cell Reports, 2022-08-30;40(9):111283.  2022-08-30
  10. Regional identity of human neural stem cells determines oncogenic responses to histone H3.3 mutants
    Authors: RB Bressan, B Southgate, KM Ferguson, C Blin, V Grant, N Alfazema, JC Wills, MA Marques-To, GM Morrison, J Ashmore, F Robertson, CAC Williams, L Bradley, A von Kriegs, RA Anderson, SR Tomlinson, SM Pollard
    Cell Stem Cell, 2021-02-24;0(0):.  2021-02-24
  11. Cell-Based Strain Remodeling of a Nonfibrous Matrix as an Organizing Principle for Vasculogenesis
    Authors: D Rüdiger, K Kick, A Goychuk, AM Vollmar, E Frey, S Zahler
    Cell Rep, 2020-08-11;32(6):108015.  2020-08-11
  12. Ythdf2-mediated m6A mRNA clearance modulates neural development in mice
    Authors: M Li, X Zhao, W Wang, H Shi, Q Pan, Z Lu, SP Perez, R Suganthan, C He, M Bjørås, A Klungland
    Genome Biol., 2018-05-31;19(1):69.  2018-05-31
  13. Coordination of epithelial branching and salivary gland lumen formation by Wnt and FGF signals.
    Authors: Patel N, Sharpe PT, Miletich I
    Dev. Biol., 2011-07-23;358(1):156-67.  2011-07-23

FAQs

  1. What type of analysis is typically applied for organoid or 3-D cell cultures?

    • Within the organoid, spheroid, or 3-D culture, cells may be assessed for morphology, apical/basal polarity, protein localization, and relative proliferation. In addition, cells may be isolated from the 3-D culture and evaluated for levels of RNA and protein expression, as well as modifications to DNA.

  2. What is the recommended working concentration for Cultrex Laminin I?

    • The recommended working concentration for thin coating is 0.05-10 µg/cm2 . However, conditions must be optimized for each cell line or model. Cultrex Laminin I should be used at 6 mg/mL for 3-D culture applications.

       

  3. What are 3-D cultures?

    • 3-D cultures are in vitro cultures where immortalized cell lines, primary cell lines, stem cells, or tissue explants are placed within hydrogel matrices, such as Cultrex Basement Membrane Extract, that mimic in vivo cell environments and allow cells to proliferate in three dimensions.

  4. What is the advantage of 3-D culture over traditional 2-D culture?

    • While 2-D culture has been used for studying many aspects of cell function and behavior, the tissue-culture treated plastic environment is unlike anything found within living organisms. As a result, cells in 2-D culture exhibit altered morphology, function, proliferation, and gene expression when compared to their emanating tissues. By placing these cells in a 3-D environment, they assume biological and biochemical characteristics similar to what is observed in vivo.

  5. What are the variables associated with 3-D culture?

    • The major variables associated with 3-D culture are cell type, cell seeding density, composition of hydrogel, thickness of hydrogel, stiffness of hydrogel, composition of cell culture medium, and time of culture.

  6. What are the different types of 3-D culture?

    • The two principal methods for performing 3-D culture are the top assay and embedded assay. For the top assay, cells are seeded on a thick gel of Cultrex Basement Membrane Extract (BME) or Extracellular Matrix Protein. A thin overlay of cell culture medium is then applied to the cells. For the embedded assay, cells are resuspended within a thick gel of Cultrex BME or ECM and the culture media is applied on top. The top assay is easier to setup, to control seeding densities, and to keep cells within one focal plane for analysis.

  7. How should cells be cultured prior to setting up the 3-D culture?

    • Cells need to be healthy and actively dividing in 2-D culture. Cells should be passaged two or three times after resuspension from cryopreservation, and they should never surpass 80% confluency during each passage. Cells should also be assessed for viability using trypan blue, and they should exhibit less than 5% staining.

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