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Cultrex HA-R-Spondin1-Fc 293T Cells

Catalog #: 3710-001-01
28 Citations 2 Reviews Datasheet / COA / SDS
1 vial (1x10^6 cell/vial)

Discontinued Product

3710-001-01 has been discontinued.
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Product Details
Citations (28)
FAQs
Reviews (2)
Summary Product Datasheets

Cultrex HA-R-Spondin1-Fc 293T Cells Summary

Stably transfected 293T cells expressing murine R-Spondin 1 that have an N-terminal HA epitope tag and are fused to a C-terminal murine IgG2a Fc fragment.

Why Use Cultrex HA-R-Spondin1-Fc 293T Cells?

Roof plate-specific Spondin-1, (R-Spondin 1, RSPO1, or CRISTIN3), is a 27 kDa secreted activator protein that belongs to the R-Spondin family. R-Spondin positively regulates Wnt/beta-catenin signaling by acting as a ligand for Lgr4-6 receptors and as an inhibitor for ZNRF3. R-Spondin 1 induces proliferation of intestinal crypt epithelial cells, increases intestinal epithelial healing, and supports intestinal epithelial stem cell renewal. Cultrex HA-R-Spondin 1-Fc 293T Cells are stably transfected to express murine R-Spondin 1 that contains an N-terminal HA epitope tag and is fused to a C-terminal murine IgG2a Fc fragment.

Cultrex HA-R-Spondin 1-Fc 293T cell line can be used to produce either purified R-Spondin 1 or R-Spondin 1-conditioned media. The murine R-Spondin 1 protein produced from this cell line has been used extensively in organoid culture to maintain Lgr5+ stem cells. The Fc and HA tags can be used for protein purification or characterization.

Kit Contents

• 1 vial (1x10^6 cell/vial) of Cultrex HA-R-Spondin1-Fc 293T Cells

Specifications

Stability
Immediately thaw for use or, for long term storage, place in vapor phase of liquid nitrogen.
Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store in liquid nitrogen for up to 12 months from the date of receipt.
Species
Mouse

Limitations

For research use only. Not for diagnostic use.

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Citations for Cultrex HA-R-Spondin1-Fc 293T Cells

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

28 Citations: Showing 1 - 10
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  1. Smart control lipid-based nanocarriers for fine-tuning gut hormone secretion
    Authors: Xu, Y;Michalowski, CB;Koehler, J;Darwish, T;Guccio, N;Alcaino, C;Domingues, I;Zhang, W;Marotti, V;Van Hul, M;Paone, P;Koutsoviti, M;Boyd, BJ;Drucker, DJ;Cani, PD;Reimann, F;Gribble, FM;Beloqui, A;
    Science advances  2024-12-13
  2. A novel approach to engineering three-dimensional bladder tumor models for drug testing
    Authors: Monjaras-Avila, CU;Luque-Badillo, AC;Bacon, JVM;Wyatt, AW;So, A;Chavez-Munoz, C;
    Scientific reports  2024-11-06
  3. Enhanced Maturity and Functionality of Vascularized Human Liver Organoids through 3D Bioprinting and Pillar Plate Culture
    Authors: Lekkala, VKR;Shrestha, S;Al Qaryoute, A;Dhinoja, S;Acharya, P;Raheem, A;Jagadeeswaran, P;Lee, MY;
    bioRxiv : the preprint server for biology  2024-08-22
  4. Isolation of Myenteric and Submucosal Plexus from Mouse Gastrointestinal Tract and Subsequent Co-Culture with Small Intestinal Organoids
    Authors: Llorente, C;
    Cells  2024-05-10
  5. Rar? -Foxa1 signaling promotes luminal identity in prostate progenitors and is disrupted in prostate cancer
    Authors: Felice, D;Alaimo, A;Bressan, D;Genovesi, S;Marmocchi, E;Annesi, N;Beccaceci, G;Dalfovo, D;Cutrupi, F;Foletto, V;Lorenzoni, M;Gandolfi, F;Kannan, S;Verma, CS;Vasciaveo, A;Shen, MM;Romanel, A;Chiacchiera, F;Cambuli, F;Lunardi, A;
    bioRxiv : the preprint server for biology  2024-03-08
  6. Establishing mouse and human oral esophageal organoids to investigate the tumor immune response
    Authors: Jiang, Y;Zhao, H;Kong, S;Zhou, D;Dong, J;Cheng, Y;Zhang, S;Wang, F;Kalra, A;Yang, N;Wei, DD;Chen, J;Zhang, YW;Lin, DC;Meltzer, SJ;Jiang, YY;
    Disease models & mechanisms  2024-01-01
  7. Traces of Canine Inflammatory Bowel Disease Reflected by Intestinal Organoids
    Authors: Pratscher, B;Kuropka, B;Csukovich, G;Doulidis, PG;Spirk, K;Kramer, N;Freund, P;Rodríguez-Rojas, A;Burgener, IA;
    International journal of molecular sciences  2024-01-01
  8. Blockage of retinoic acid signaling via RAR? suppressed the proliferation of pancreatic cancer cells by arresting the cell cycle progression of the G1-S phase
    Authors: Yamakawa, K;Koyanagi-Aoi, M;Machinaga, A;Kakiuchi, N;Hirano, T;Kodama, Y;Aoi, T;
    Cancer cell international  2023-05-17
  9. Skin basal cell carcinomas assemble a pro-tumorigenic spatially organized and self-propagating Trem2+ myeloid niche
    Authors: Haensel, D;Daniel, B;Gaddam, S;Pan, C;Fabo, T;Bjelajac, J;Jussila, AR;Gonzalez, F;Li, NY;Chen, Y;Hou, J;Patel, T;Aasi, S;Satpathy, AT;Oro, AE;
    Nature communications  2023-05-10
  10. Trophozoite fitness dictates the intestinal epithelial cell response to Giardia intestinalis infection
    Authors: Gr�ttner, J;van Rijn, JM;Geiser, P;Florbrant, A;Webb, DL;Hellstr�m, PM;Sundbom, M;Sellin, ME;Sv�rd, SG;
    PLoS pathogens  2023-05-01
  11. A DLG1-ARHGAP31-CDC42 axis is essential for the intestinal stem cell response to fluctuating niche Wnt signaling
    Authors: D Castillo-A, T Wald, EA Reyes, A Gallagher, J Schanin, S Vlachos, N Lamarche-V, C Bomidi, S Blutt, MK Estes, T Nystul, OD Klein
    Cell Stem Cell, 2023-01-13;30(2):188-206.e6.  2023-01-13
  12. Disruption of the circadian clock drives Apc loss of heterozygosity to accelerate colorectal cancer
    Authors: SK Chun, BM Fortin, RC Fellows, AN Habowski, A Verlande, WA Song, AL Mahieu, AEYT Lefebvre, JN Sterrenber, LM Velez, MA Digman, RA Edwards, NR Pannunzio, MM Seldin, ML Waterman, S Masri
    Science Advances, 2022-08-10;8(32):eabo2389.  2022-08-10
  13. Glucocorticoids enhance chemotherapy-driven stress granule assembly and impair granule dynamics leading to cell death
    Authors: A Schwed-Gro, H Hamiel, GP Faber, M Angel, R Ben-Yishay, JIC Benichou, D Ishay-Rone, Y Shav-Tal
    Journal of Cell Science, 2022-07-26;0(0):.  2022-07-26
  14. Deciphering the immunopeptidome in vivo reveals new tumour antigens
    Authors: AM Jaeger, LE Stopfer, R Ahn, EA Sanders, DA Sandel, WA Freed-Past, WM Rideout, S Naranjo, T Fessenden, KB Nguyen, PS Winter, RE Kohn, PMK Westcott, JM Schenkel, SL Shanahan, AK Shalek, S Spranger, FM White, T Jacks
    Nature, 2022-06-15;0(0):.  2022-06-15
  15. The role of Jagged1 as a dynamic switch of cancer cell plasticity in PDAC assembloids
    Authors: JI Choi, JH Rim, SI Jang, JS Park, H Park, JH Cho, JB Lim
    Theranostics, 2022-05-24;12(9):4431-4445.  2022-05-24
  16. RNA splicing is a key mediator of tumour cell plasticity and a therapeutic vulnerability in colorectal cancer
    Authors: AE Hall, SÖ Pohl, P Cammareri, S Aitken, NT Younger, M Raponi, CV Billard, AB Carrancio, A Bastem, P Freile, F Haward, IR Adams, JF Caceres, P Preyzner, A von Kriegs, MG Dunlop, FV Din, KB Myant
    Nature Communications, 2022-05-19;13(1):2791.  2022-05-19
  17. Modeling Pancreatic Cancer with Patient-Derived Organoids Integrating Cancer-Associated Fibroblasts
    Authors: YH Go, WH Choi, WJ Bae, SI Jung, CH Cho, SA Lee, JS Park, JM Ahn, SW Kim, KJ Lee, D Lee, J Yoo
    Cancers, 2022-04-21;14(9):.  2022-04-21
  18. Suspension culture promotes serosal mesothelial development in human intestinal organoids
    Authors: MM Capeling, S Huang, CJ Childs, JH Wu, YH Tsai, A Wu, N Garg, EM Holloway, N Sundaram, C Bouffi, M Helmrath, JR Spence
    Cell Reports, 2022-02-15;38(7):110379.  2022-02-15
  19. DKK1 inhibits canonical Wnt signaling in human papillomavirus-positive penile cancer cells
    Authors: IA Bley, A Zwick, MC Hans, K Thieser, V Wagner, N Ludwig, O Khalmurzae, VB Matveev, P Loertzer, A Pryalukhin, A Hartmann, CI Geppert, H Loertzer, H Wunderlich, CM Naumann, H Kalthoff, K Junker, S Smola, S Lohse
    Translational Oncology, 2021-11-10;15(1):101267.  2021-11-10
  20. Inflation-collapse dynamics drive patterning and morphogenesis in intestinal organoids
    Authors: NP Tallapraga, HM Cambra, T Wald, S Keough Jal, DM Abraham, OD Klein, AM Klein
    Cell Stem Cell, 2021-04-28;0(0):.  2021-04-28
  21. Human gastrointestinal epithelia of the esophagus, stomach, and duodenum resolved at single-cell resolution
    Authors: GA Busslinger, BLA Weusten, A Bogte, H Begthel, LAA Brosens, H Clevers
    Cell Reports, 2021-03-09;34(10):108819.  2021-03-09
  22. Short-term organoid culture for drug sensitivity testing of high-grade serous carcinoma
    Authors: H Chen, K Gotimer, C De Souza, CG Tepper, AN Karnezis, GS Leiserowit, J Chien, LH Smith
    Gynecol. Oncol., 2020-04-04;0(0):.  2020-04-04
  23. Generation of Differentiating and Long-Living Intestinal Organoids Reflecting the Cellular Diversity of Canine Intestine
    Authors: N Kramer, B Pratscher, AMC Meneses, W Tschulenk, I Walter, A Swoboda, HS Kruitwagen, K Schneeberg, LC Penning, B Spee, M Kieslinger, S Brandt, IA Burgener
    Cells, 2020-03-28;9(4):.  2020-03-28
  24. Distinct Mesenchymal Cell Populations Generate the Essential Intestinal BMP Signaling Gradient
    Authors: N McCarthy, E Manieri, EE Storm, A Saadatpour, AM Luoma, VN Kapoor, S Madha, LT Gaynor, C Cox, S Keerthivas, K Wucherpfen, GC Yuan, FJ de Sauvage, SJ Turley, RA Shivdasani
    Cell Stem Cell, 2020-02-20;0(0):.  2020-02-20
  25. Butyric Acid and Leucine Induce alpha-Defensin Secretion from Small Intestinal Paneth Cells
    Authors: A Takakuwa, K Nakamura, M Kikuchi, R Sugimoto, S Ohira, Y Yokoi, T Ayabe
    Nutrients, 2019-11-18;11(11):.  2019-11-18
  26. Development of Collagen-Based 3D Matrix for Gastrointestinal Tract-Derived Organoid Culture
    Authors: JH Jee, DH Lee, J Ko, S Hahn, SY Jeong, HK Kim, E Park, SY Choi, S Jeong, JW Lee, HJ Cho, MS Kwon, J Yoo
    Stem Cells Int, 2019-06-13;2019(0):8472712.  2019-06-13
  27. Paneth cell granule dynamics on secretory responses to bacterial stimuli in enteroids
    Authors: Y Yokoi, K Nakamura, T Yoneda, M Kikuchi, R Sugimoto, Y Shimizu, T Ayabe
    Sci Rep, 2019-02-25;9(1):2710.  2019-02-25
  28. Direct therapeutic targeting of immune checkpoint PD-1 in pancreatic cancer
    Authors: M Gao, M Lin, RA Moffitt, MA Salazar, J Park, J Vacirca, C Huang, KR Shroyer, M Choi, GV Georgakis, AR Sasson, MA Talamini, J Kim
    Br. J. Cancer, 2018-10-31;120(1):88-96.  2018-10-31

FAQs

  1. Murine R-Spondin 1 is reported to be a 27kDa protein, but the protein produced by the 293T cells in Catalog # 3710-001-01  is 70-75 kDa. Why?

    • The natural R-Spondin 1 protein is approximately 27 kDa. The cells in this product express a construct containing both an additional HA-tag plus an Fc fragment, hence the much larger size.

  2. For culturing and handling of the Cultrex HA-R-Spondin1-Fc 293T Cells (catalog # 3710-001-01), does the Fetal Bovine Serum need to be heat-inactivated in the Basal Growth Medium or Freeze Medium?

    • Heat-inactivated serum is not required for either of these media.

  3. Has this cell line been tested for mycoplasma?

    • Yes, all retail lots are tested to confirm mycoplasma is undetected by PCR, which is documented on the lot-specific CoA.

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Reviews for Cultrex HA-R-Spondin1-Fc 293T Cells

Average Rating: 5 (Based on 2 Reviews)

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Cultrex HA-R-Spondin1-Fc 293T Cells
By Anonymous on 12/28/2020

For organoid culture

Cultrex HA-R-Spondin1-Fc 293T Cells 3710-001-01
Cultrex HA-R-Spondin1-Fc 293T Cells
By Anonymous on 02/05/2019
Application: generation of organoids for in vivo studies
Reason for Rating: Cells produced R-spondin

Used to confirm activation of Wnt signalling