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Cultrex Reduced Growth Factor Basement Membrane Extract, Type 2, Pathclear

Catalog #: 3533-010-02
61 Citations 5 Reviews Datasheet / COA / SDS
For Robust Organoid Cultures - Reduced Growth Factor Basement Membrane Extract
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Mouse Intestinal Organoids Cultured in Cultrex RGF BME Type 2_3533-005-02
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Cultrex Reduced Growth Factor Basement Membrane Extract, Type 2, Pathclear Summary

Cultrex Reduced Growth Factor Basement Membrane Extract (RGF BME ),Type 2 is specifically qualified to support the establishment and expansion of robust organoid cultures. It's composition mimics the in vivo microenvironment to improve take rate and growth of organoids.
 

Key Benefits

• Qualified for use in organoid cell culture
• Commonly used robust and established organoid systems
• Reduced growth factor formulation provides a more defined culture system
• Quality controlled for performance consistency

Why Use Cultrex RGF Basement Membrane Extract, Type 2?

Cultrex Reduced Growth Factor Basement Membrane Extract (RGF BME), Type 2 is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. This extract provides a natural extracellular matrix hydrogel that polymerizes at 37°C to form a reconstituted basement membrane. Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. The major components of BME include laminin, collagen IV, entactin, and heparan sulfate proteoglycans.

Cultrex RGF BME, Type 2 provides a proprietary formulation that has a high storage modulus and is designed for use in robust tissue organoid culture as well as other applications requiring an extracellular matrix scaffold.

Protocols Utilizing Cultrex RGF Basement Membrane Extract, Type 2 for Organoid Cell Culture.

Cultrex RGF BME, Type 2 is ideal for use as a scaffold for organoid and 3D cell culture. Listed below are protocols designed by our research and development groups for different types of organoids featuring Cultrex RGF BME, Type 2 as well as growth factors, media supplements, and small molecules from Bio-Techne.
Video of Cultrex BME Best Practices and Protocols.
Protocol for Mouse Enteric Organoid Culture.
Protocol for Human Gastric Organoid Culture.
Protocol for Human Liver Organoid Culture.
Protocol for Human Lung Organoid Culture.
Protocol for Human Intestinal Organoid Culture.
Protocol for Harvesting Organoids for Biochemical Analysis.

Specifications

Source
Murine Engelbreth-Holm-Swarm (EHS) tumor
Sterility Testing
No bacterial or fungal growth detected following 14 days in culture
Testing Cell Culture
Organoid Culture - Cultrex RGF BME, Type 2 supports growth and expansion of mouse small intestine organoid progenitor cells.

Gelling Assay - Cultrex RGF BME, Type 2 gels in less than 30 minutes at 37 °C, and maintains the gelled form in culture medium for a minimum of 7 days at 37 °C.

Dome Assay Cultrex RGF BME, Type 2 forms and maintains stable 3-D dome structures on cell culture plates.

Tube Formation Assay - Cultrex RGF BME, Type 2 supports formation of capillary-like structures by human (HBMVEC; HUVEC) or mouse (SVEC4-10) endothelial cells.
Viral Testing
Tested negative by PCR test for a total of 31 organisms and viruses, including: mycoplasma, 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, and 13 additional murine infectious agents including LDEV.
Stability
Product is stable for at least two years from date of manufacture when stored at ≤ -70 °C. See lot specific Certificate of Analysis for expiration date.
Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Species
Mouse

Limitations

For research use only. Not for diagnostic use.

Product Datasheets

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Scientific Data

N/A Mouse Intestinal Organoids Cultured in Cultrex RGF BME Type 2_3533-005-02 View Larger

Mouse Intestinal Organoids Cultured in Cultrex RGF BME, Type 2. Mouse intestinal organoids cultured in Cultrex RGF BME, Type 2 were immersion fixed and processed for whole mount staining or paraffin embedding and sectioning for immunocytochemistry. A) Whole mount mouse intestinal organoids were stained using Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (green; Catalog # AF748) at 10 µg/mL for 3 hours at room temperature. Cells were counterstained with DAPI (blue). B) Paraffin-embedded mouse intestinal tissue stained for Human Cadherin‑17 Antibody (green; Catalog # MAB1032), Human Ki67/MKI67 Antibody (red; Catalog # AF7617), and counterstained with DAPI (blue).

N/A Human Lung Organoids Cultured in Cultrex RGF BME, Type 2 View Larger

Human Lung Organoids Cultured in Cultrex RGF BME, Type 2. A) Representative brightfield image of human lung organoids cultured using Cultrex RGF BME, Type 2. B) Expression of Sox2 (green; Catalog # AF2018) and Acetylated Tubulin (red; Novus Biologicals, Catalog # NB600-567). C) Expression of p63/TP73L (green; Catalog # AF1916) and Cytokeratin 10 (red; Novus Biologicals, Catalog # NBP2-61736). D) Expression of Podoplanin (green; Catalog # AF3670) as a marker of type 1 alveolar cells.

N/A Liver Organoid Formation and Differentiation in Cultrex RGF BME View Larger

Liver Organoid Formation and Differentiation in Cultrex RGF BME, Type 2. Human Liver organoids were derived from human biopsy tissue. Undifferentiated organoids were formed by embedding dissociated tissue in Cultrex RGF BME, Type 2 and culturing in specialized media. The organoids were differentiated using media containing Recombinant Human FGF-19 (Catalog # 969-FG), DAPT (Catalog # 2634), and Dexamethasone (Catalog # 1126). A) Undifferentiated liver organoids. B) Liver organoids shrink as they differentiate. C) Representative images of differentiated liver organoids expression hepatocyte markers, Albumin and HNF3 beta, as well as E-Cadherin.

N/A Human Intestinal Organoids Cultured using Cultrex RGF BME View Larger

Human Intestinal Organoids Cultured using Cultrex RGF BME, Type 2. Human transverse colon organoids (A,B) and human ileum organoids (C, D) were grown using cells isolated from transverse colon and ileum biopsy tissue, respectively. Organoids were embedded in Cultrex RGF BME, Type 2 as a scaffold matrix. A) Brightfield image of human transverse organoids. B) Human transverse organoid stained using Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (green; Catalog # AF748), a MUC2 Antibody (red; Catalog # NBP2-44431), and DAPI (blue). C) Brightfield image of human ileum organoids. D) Human ileum organoid stained using a Aldolase B Antibody (red; Catalog # NBP2-15345), a Human Cadherin-17 Antibody (green; Catalog # MAB1032, and DAPI (blue).

N/A HepG2 Spheroids in Cultrex RGF BME, Type 2 View Larger

Formation of HepG2 Spheroids in Cultrex RGF BME, Type 2. Hepatocyte spheroids were formed by plating HepG2 liver hepatocellular carcinoma cells (10,000 cells per well) in a 24-well plate coated with 5 mg/mL of Cultrex RGF BME, Type 2. Spheroids were cultured for 21 days prior imaging by brightfield microscopy.

Citations for Cultrex Reduced Growth Factor Basement Membrane Extract, Type 2, Pathclear

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

61 Citations: Showing 1 - 10
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  1. NUAK1 activates STAT5/GLI1/SOX2 signaling to enhance cancer cell expansion and drives chemoresistance in gastric cancer
    Authors: Cao, L;Lin, G;Fan, D;Weng, K;Chen, Y;Wang, J;Li, P;Zheng, C;Huang, C;Xie, J;
    Cell reports  2024-07-23
  2. A YAP-centered mechanotransduction loop drives collective breast cancer cell invasion
    Authors: Khalil, AA;Smits, D;Haughton, PD;Koorman, T;Jansen, KA;Verhagen, MP;van der Net, M;van Zwieten, K;Enserink, L;Jansen, L;El-Gammal, AG;Visser, D;Pasolli, M;Tak, M;Westland, D;van Diest, PJ;Moelans, CB;Roukens, MG;Tavares, S;Fortier, AM;Park, M;Fodde, R;Gloerich, M;Zwartkruis, FJT;Derksen, PW;de Rooij, J;
    Nature communications  2024-06-07
  3. Molecular Characterization and Therapeutic Opportunities in KRAS Wildtype Pancreatic Ductal Adenocarcinoma
    Authors: Desai, A;Xiao, AH;Choi, D;Toruner, MD;Walden, D;Halfdanarson, TR;Alberts, S;McWilliams, RR;Mahipal, A;Ahn, D;Babiker, H;Stybayeva, G;Revzin, A;Kizilbash, S;Adjei, A;Bekaii-Saab, T;Mansfield, AS;Carr, RM;Ma, WW;
    Cancers  2024-05-13
  4. Enhancing therapeutic efficacy in luminal androgen receptor triple-negative breast cancer: exploring chidamide and enzalutamide as a promising combination strategy
    Authors: Zhao, YX;Wang, H;Zhang, SW;Zhang, WX;Jiang, YZ;Shao, ZM;
    Cancer cell international  2024-04-09
  5. Renin-Angiotensin Inhibitor, Captopril, Attenuates Growth of Patient-Derived Colorectal Liver Metastasis Organoids
    Authors: Riddiough, GE;Fifis, T;Muralidharan, V;Christophi, C;Tran, BM;Perini, MV;Vincan, E;
    International journal of molecular sciences  2024-03-14
  6. Sox9 links biliary maturation to branching morphogenesis
    Authors: Hrncir, HR;Bombin, S;Goodloe, B;Hogan, CB;Jadi, O;Gracz, AD;
    bioRxiv : the preprint server for biology  2024-01-16
  7. Urine-derived bladder cancer organoids (urinoids) as a tool for cancer longitudinal response monitoring and therapy adaptation
    Authors: Viergever, BJ;Raats, DAE;Geurts, V;Mullenders, J;Jonges, TN;van der Heijden, MS;van Es, JH;Kranenburg, O;Meijer, RP;
    British journal of cancer  2023-12-15
  8. STAT2 Controls Colorectal Tumorigenesis and Resistance to Anti-Cancer Drugs
    Authors: Chiriac, MT;Hracsko, Z;Becker, C;Neurath, MF;
    Cancers  2023-11-15
  9. Functional screening of amplification outlier oncogenes in organoid models of early tumorigenesis
    Authors: Salahudeen, AA;Seoane, JA;Yuki, K;Mah, AT;Smith, AR;Kolahi, K;De la O, SM;Hart, DJ;Ding, J;Ma, Z;Barkal, SA;Shukla, ND;Zhang, CH;Cantrell, MA;Batish, A;Usui, T;Root, DE;Hahn, WC;Curtis, C;Kuo, CJ;
    Cell reports  2023-11-01
  10. Luminal breast cancer identity is determined by loss of glucocorticoid receptor activity
    Authors: Prekovic, S;Chalkiadakis, T;Roest, M;Roden, D;Lutz, C;Schuurman, K;Opdam, M;Hoekman, L;Abbott, N;Tesselaar, T;Wajahat, M;Dwyer, AR;Mayayo-Peralta, I;Gomez, G;Altelaar, M;Beijersbergen, R;Gy?rffy, B;Young, L;Linn, S;Jonkers, J;Tilley, W;Hickey, T;Vareslija, D;Swarbrick, A;Zwart, W;
    EMBO molecular medicine  2023-10-30
  11. Single-nucleus RNA sequencing reveals heterogenous microenvironments and specific drug response between cervical squamous cell carcinoma and adenocarcinoma
    Authors: Lin, S;Sun, Y;Cao, C;Zhu, Z;Xu, Y;Liu, B;Hu, B;Peng, T;Zhi, W;Xu, M;Ding, W;Ren, F;Ma, D;Li, G;Wu, P;
    EBioMedicine  2023-10-23
  12. KLHL29-mediated DDX3X degradation promotes chemosensitivity by abrogating cell cycle checkpoint in triple-negative breast cancer
    Authors: Yao, L;Hao, Q;Wang, M;Chen, Y;Cao, H;Zhang, Q;Yu, K;Jiang, Y;Shao, Z;Zhou, X;Xu, Y;
    Oncogene  2023-10-16
  13. Patient-derived organoid culture in epithelial ovarian cancers-Techniques, applications, and future perspectives
    Authors: Chan, WS;Mo, X;Ip, PPC;Tse, KY;
    Cancer medicine  2023-09-30
  14. Jaw Bone Invasion of Oral Squamous Cell Carcinoma Is Associated with Osteoclast Count and Expression of Its Regulating Proteins in Patients and Organoids
    Authors: de Kort, WWB;Haakma, WE;van Es, RJJ;Gawlitta, D;Driehuis, E;Gansevoort, M;Willems, SM;
    Journal of clinical medicine  2023-09-18
  15. Activation of invasion by oncogenic reprogramming of cholesterol metabolism via increased NPC1 expression and macropinocytosis
    Authors: Skorda, A;Lauridsen, AR;Wu, C;Huang, J;Mrackova, M;Winther, NI;Jank, V;Sztupinszki, Z;Strauss, R;Bilgin, M;Maeda, K;Liu, B;Luo, Y;Jäättelä, M;Kallunki, T;
    Oncogene  2023-07-07
  16. Immune regulatory function of cancer-associated fibroblasts in non-small cell lung cancer
    Authors: Lee, H;Hwang, M;Jang, S;Um, SW;
    Tuberculosis and respiratory diseases  2023-06-22
  17. Creation of EGD-Derived Gastric Cancer Organoids to Predict Treatment Responses
    Authors: McDonald, HG;Harper, MM;Hill, K;Gao, A;Solomon, AL;Bailey, CJ;Lin, M;Barry-Hundeyin, M;Cavnar, MJ;Mardini, SH;Pandalai, PJ;Patel, RA;Kolesar, JM;Rueckert, JA;Hookey, L;Ropeleski, M;Merchant, SJ;Kim, J;Gao, M;
    Cancers  2023-06-02
  18. Multi-omics analysis reveals distinct non-reversion mechanisms of PARPi resistance in BRCA1- versus BRCA2-deficient mammary tumors
    Authors: Bhin, J;Paes Dias, M;Gogola, E;Rolfs, F;Piersma, SR;de Bruijn, R;de Ruiter, JR;van den Broek, B;Duarte, AA;Sol, W;van der Heijden, I;Andronikou, C;Kaiponen, TS;Bakker, L;Lieftink, C;Morris, B;Beijersbergen, RL;van de Ven, M;Jimenez, CR;Wessels, LFA;Rottenberg, S;Jonkers, J;
    Cell reports  2023-05-19
  19. A histone deacetylase 3 and mitochondrial complex I axis regulates toxic formaldehyde production
    Authors: Wit, N;Gogola, E;West, JA;Vornb�umen, T;Seear, RV;Bailey, PSJ;Burgos-Barragan, G;Wang, M;Krawczyk, P;Huberts, DHEW;Gergely, F;Matheson, NJ;Kaser, A;Nathan, JA;Patel, KJ;
    Science advances  2023-05-19
  20. ONECUT2 regulates RANKL-dependent enterocyte and microfold cell differentiation in the small intestine; a multi-omics study
    Authors: MV Luna Velez, HK Neikes, RR Snabel, Y Quint, C Qian, A Martens, GJC Veenstra, MR Freeman, SJ van Heerin, M Vermeulen
    Nucleic Acids Research, 2023-02-22;0(0):.  2023-02-22
  21. Mutated axon guidance gene PLXNB2 sustains growth and invasiveness of stem cells isolated from cancers of unknown primary
    Authors: S Brundu, V Napolitano, G Franzolin, E Lo Cascio, R Mastranton, G Sardo, E Cascardi, F Verginelli, S Sarnataro, G Gambardell, A Pisacane, A Arcovito, C Boccaccio, PM Comoglio, E Giraudo, L Tamagnone
    Embo Molecular Medicine, 2023-02-01;0(0):e16104.  2023-02-01
  22. Hypoxia-Driven Changes in a Human Intestinal Organoid Model and the Protective Effects of Hydrolyzed Whey
    Authors: IH de Lange, C van Gorp, KRI Massy, L Kessels, N Kloosterbo, A Bjørnshave, M Stampe Ost, JGMC Damoiseaux, JPM Derikx, WG van Gemert, TGAM Wolfs
    Nutrients, 2023-01-12;15(2):.  2023-01-12
  23. Metabolic Activation of Benzo[a]pyrene by Human Tissue Organoid Cultures
    Authors: AL Caipa Garc, JE Kucab, H Al-Serori, RSS Beck, F Fischer, M Hufnagel, A Hartwig, A Floeder, S Balbo, H Francies, M Garnett, M Huch, J Drost, M Zilbauer, VM Arlt, DH Phillips
    International Journal of Molecular Sciences, 2022-12-29;24(1):.  2022-12-29
  24. AKTIP loss is enriched in ERalpha-positive breast cancer for tumorigenesis and confers endocrine resistance
    Authors: ASN Ng, S Zhang, VCY Mak, Y Zhou, Y Yuen, R Sharma, Y Lu, G Zhuang, W Zhao, HH Pang, LWT Cheung
    Cell Reports, 2022-12-13;41(11):111821.  2022-12-13
  25. Metastatic recurrence in colorectal cancer arises from residual EMP1+ cells
    Authors: A Cañellas-S, C Cortina, X Hernando-M, S Palomo-Pon, EJ Mulholland, G Turon, L Mateo, S Conti, O Roman, M Sevillano, F Slebe, D Stork, A Caballé-Me, A Berenguer-, A Álvarez-Va, N Fenderico, L Novellasde, L Jiménez-Gr, T Sipka, L Bardia, P Lorden, J Colombelli, H Heyn, X Trepat, S Tejpar, E Sancho, DVF Tauriello, S Leedham, CS Attolini, E Batlle
    Nature, 2022-11-09;611(7936):603-613.  2022-11-09
  26. Computational pharmacogenomic screen identifies drugs that potentiate the anti-breast cancer activity of statins
    Authors: JE van Leeuwe, W Ba-Alawi, E Branchard, J Cruickshan, W Schormann, J Longo, J Silvester, PL Gross, DW Andrews, DW Cescon, B Haibe-Kain, LZ Penn, DMA Gendoo
    Nature Communications, 2022-10-24;13(1):6323.  2022-10-24
  27. Division of labor within the DNA damage tolerance system reveals non-epistatic and clinically actionable targets for precision cancer medicine
    Authors: A Spanjaard, R Shah, D de Groot, OA Buoninfant, B Morris, C Lieftink, C Pritchard, LM Zürcher, S Ormel, JJI Catsman, R de Korte-G, B Siteur, N Proost, T Boadum, M van de Ven, JY Song, M Kreft, PCM van den Be, RL Beijersber, H Jacobs
    Nucleic Acids Research, 2022-07-22;50(13):7420-7435.  2022-07-22
  28. The metastatic spread of breast cancer accelerates during sleep
    Authors: Z Diamantopo, F Castro-Gin, FD Schwab, C Foerster, M Saini, S Budinjas, K Strittmatt, I Krol, B Seifert, V Heinzelman, C Kurzeder, C Rochlitz, M Vetter, WP Weber, N Aceto
    Nature, 2022-06-22;607(7917):156-162.  2022-06-22
  29. RNA splicing is a key mediator of tumour cell plasticity and a therapeutic vulnerability in colorectal cancer
    Authors: AE Hall, SÖ Pohl, P Cammareri, S Aitken, NT Younger, M Raponi, CV Billard, AB Carrancio, A Bastem, P Freile, F Haward, IR Adams, JF Caceres, P Preyzner, A von Kriegs, MG Dunlop, FV Din, KB Myant
    Nature Communications, 2022-05-19;13(1):2791.  2022-05-19
  30. Human branching cholangiocyte organoids recapitulate functional bile duct formation
    Authors: FJM Roos, GS van Tiende, H Wu, I Bordeu, D Vinke, LM Albarinos, K Monfils, S Niesten, R Smits, J Willemse, O Rosmark, G Westergren, DJ Kunz, M de Wit, PJ French, L Vallier, JNM IJzermans, R Bartfai, H Marks, BD Simons, ME van Royen, MMA Verstegen, LJW van der La
    Cell Stem Cell, 2022-05-05;29(5):776-794.e13.  2022-05-05
  31. Cell-intrinsic Aryl Hydrocarbon Receptor signalling is required for the resolution of injury-induced colonic stem cells
    Authors: K Shah, MR Maradana, M Joaquina D, A Metidji, F Graelmann, M Llorian, P Chakravart, Y Li, M Tolaini, M Shapiro, G Kelly, C Cheshire, D Bhurta, SB Bharate, B Stockinger
    Nature Communications, 2022-04-05;13(1):1827.  2022-04-05
  32. Copy number amplification of ENSA promotes the progression of triple-negative breast cancer via cholesterol biosynthesis
    Authors: YY Chen, JY Ge, SY Zhu, ZM Shao, KD Yu
    Nature Communications, 2022-02-10;13(1):791.  2022-02-10
  33. Comprehensive metabolomics expands precision medicine for triple-negative breast cancer
    Authors: Y Xiao, D Ma, YS Yang, F Yang, JH Ding, Y Gong, L Jiang, LP Ge, SY Wu, Q Yu, Q Zhang, F Bertucci, Q Sun, X Hu, DQ Li, ZM Shao, YZ Jiang
    Cell Research, 2022-02-01;0(0):.  2022-02-01
  34. Efficient and error-free fluorescent gene tagging in human organoids without double-strand DNA cleavage
    Authors: Y Bollen, JH Hageman, P van Leenen, LLM Derks, B Ponsioen, JR Buissant d, I Verlaan-Kl, M van den Bo, LWMM Terstappen, R van Boxtel, HJG Snippert
    PloS Biology, 2022-01-28;20(1):e3001527.  2022-01-28
  35. Lineage-specific silencing of PSAT1 induces serine auxotrophy and sensitivity to dietary serine starvation in luminal breast tumors
    Authors: BH Choi, V Rawat, J Högström, PA Burns, KO Conger, ME Ozgurses, JM Patel, TS Mehta, A Warren, LM Selfors, T Muranen, JL Coloff
    Cell Reports, 2022-01-18;38(3):110278.  2022-01-18
  36. Air-Liquid-Interface Differentiated Human Nose Epithelium: A Robust Primary Tissue Culture Model of SARS-CoV-2 Infection
    Authors: BM Tran, SL Grimley, JL McAuley, A Hachani, L Earnest, SL Wong, L Caly, J Druce, DFJ Purcell, DC Jackson, M Catton, CJ Nowell, L Leonie, G Deliyannis, SA Waters, J Torresi, E Vincan
    International Journal of Molecular Sciences, 2022-01-13;23(2):.  2022-01-13
  37. Adult mouse and human organoids derived from thyroid follicular cells and modeling of Graves' hyperthyroidism
    Authors: J van der Va, L Bosmans, SF Sijbesma, K Knoops, WJ van de Wet, HG Otten, H Begthel, IHM Borel Rink, J Korving, EGWM Lentjes, C Lopez-Igle, PJ Peters, HM van Santen, MR Vriens, H Clevers
    Proceedings of the National Academy of Sciences of the United States of America, 2021-12-21;118(51):.  2021-12-21
  38. A Bioluminescent 3CLPro Activity Assay to Monitor SARS-CoV-2 Replication and Identify Inhibitors
    Authors: C Mathieu, F Touret, C Jacquemin, YL Janin, A Nougairède, M Brailly, M Mazelier, D Décimo, V Vasseur, A Hans, JC Valle-Casu, X de Lamball, B Horvat, P André, M Si-Tahar, V Lotteau, PO Vidalain
    Viruses, 2021-09-12;13(9):.  2021-09-12
  39. Organoid-based drug screening reveals neddylation as therapeutic target for malignant rhabdoid tumors
    Authors: C Calandrini, SR van Hooff, I Paassen, D Ayyildiz, S Derakhshan, MEM Dolman, KPS Langenberg, M van de Ven, C de Heus, N Liv, M Kool, RR de Krijger, GAM Tytgat, MM van den He, JJ Molenaar, J Drost
    Cell Reports, 2021-08-24;36(8):109568.  2021-08-24
  40. Ultrastructural analysis of breast cancer patient-derived organoids
    Authors: L Signati, R Allevi, F Piccotti, S Albasini, L Villani, M Sevieri, A Bonizzi, F Corsi, S Mazzucchel
    Cancer Cell International, 2021-08-10;21(1):423.  2021-08-10
  41. ERBB3 overexpression due to miR-205 inactivation confers sensitivity to FGF, metabolic activation, and liability to ERBB3 targeting in glioblastoma
    Authors: F De Bacco, F Orzan, J Erriquez, E Casanova, L Barault, R Albano, A D'Ambrosio, V Bigatto, G Reato, M Patanè, B Pollo, G Kuesters, C Dell'Aglio, L Casorzo, S Pellegatta, G Finocchiar, PM Comoglio, C Boccaccio
    Cell Reports, 2021-07-27;36(4):109455.  2021-07-27
  42. RAC1B modulates intestinal tumourigenesis via modulation of WNT and EGFR signalling pathways
    Authors: V Gudiño, SÖ Pohl, CV Billard, P Cammareri, A Bolado, S Aitken, D Stevenson, AE Hall, M Agostino, J Cassidy, C Nixon, A von Kriegs, P Freile, L Popplewell, G Dickson, L Murphy, A Wheeler, M Dunlop, F Din, D Strathdee, OJ Sansom, KB Myant
    Nature Communications, 2021-04-20;12(1):2335.  2021-04-20
  43. Culture and analysis of kidney tubuloids and perfused tubuloid cells-on-a-chip
    Authors: L Gijzen, FA Yousef Yen, F Schutgens, MK Vormann, CME Ammerlaan, A Nicolas, D Kurek, P Vulto, MB Rookmaaker, HL Lanz, MC Verhaar, H Clevers
    Nature Protocols, 2021-03-05;0(0):.  2021-03-05
  44. Inhibition of mitochondrial function by metformin increases glucose uptake, glycolysis and GDF-15 release from intestinal cells
    Authors: M Yang, T Darwish, P Larraufie, D Rimmington, I Cimino, DA Goldspink, B Jenkins, A Koulman, CA Brighton, M Ma, BYH Lam, AP Coll, S O'Rahilly, F Reimann, FM Gribble
    Scientific Reports, 2021-01-28;11(1):2529.  2021-01-28
  45. Sox9EGFP defines biliary epithelial heterogeneity downstream of Yap activity
    Authors: DY Tulasi, DM Castaneda, K Wager, CB Hogan, KP Alcedo, JR Raab, AD Gracz
    Cellular and Molecular Gastroenterology and Hepatology, 2021-01-23;0(0):.  2021-01-23
  46. Loss of sphingosine 1-phosphate receptor 3 gene function impairs injury-induced stromal angiogenesis in mouse cornea
    Authors: S Yasuda, T Sumioka, H Iwanishi, Y Okada, M Miyajima, K Ichikawa, PS Reinach, S Saika
    Lab Invest, 2020-11-16;0(0):.  2020-11-16
  47. Single-cell derived tumor organoids display diversity in HLA class I peptide presentation
    Authors: LC Demmers, K Kretzschma, A Van Hoeck, YE Bar-Epraïm, HWP van den To, M Koomen, G van Son, J van Gorp, A Pronk, N Smakman, E Cuppen, H Clevers, AJR Heck, W Wu
    Nat Commun, 2020-10-21;11(1):5338.  2020-10-21
  48. Hypoxia Triggers the Intravasation of Clustered Circulating Tumor Cells
    Authors: C Donato, L Kunz, F Castro-Gin, A Paasinen-S, K Strittmatt, BM Szczerba, R Scherrer, N Di Maggio, W Heusermann, O Biehlmaier, C Beisel, M Vetter, C Rochlitz, WP Weber, A Banfi, T Schroeder, N Aceto
    Cell Rep, 2020-09-08;32(10):108105.  2020-09-08
  49. Functional Radiogenetic Profiling Implicates ERCC6L2 in Non-homologous End Joining
    Authors: P Francica, M Mutlu, VA Blomen, C Oliveira, Z Nowicka, A Trenner, NM Gerhards, P Bouwman, E Stickel, ML Hekkelman, L Lingg, I Klebic, M van de Ven, R de Korte-G, D Howald, J Jonkers, AA Sartori, W Fendler, JR Chapman, T Brummelkam, S Rottenberg
    Cell Rep, 2020-08-25;32(8):108068.  2020-08-25
  50. Patient-derived oral mucosa organoids as an in vitro model for methotrexate induced toxicity in pediatric acute lymphoblastic leukemia
    Authors: E Driehuis, N Oosterom, SG Heil, IB Muller, M Lin, S Kolders, G Jansen, R de Jonge, R Pieters, H Clevers, MM van den He
    PLoS ONE, 2020-05-18;15(5):e0231588.  2020-05-18
  51. Slug-expressing mouse prostate epithelial cells have increased stem cell potential
    Authors: Z Kahounová, J Remšík, R Fedr, J Bouchal, A Mi?ková, E Slabáková, L Binó, A Hampl, K Sou?ek
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    Authors: JM Rosenbluth, RCJ Schackmann, GK Gray, LM Selfors, CM Li, M Boedicker, HJ Kuiken, A Richardson, J Brock, J Garber, D Dillon, N Sachs, H Clevers, JS Brugge
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FAQs

  1. What kinds of tumor cells or biopsy specimens grow in vivo with Cultrex® BME?

    • Many cell lines and tumor biopsy specimens (usually cut into small fragments) have been found to grow in vivo when implanted with Cultrex® BME. These include melanoma, intestinal, prostate, breast, lung, renal, and liver cancers as well as the 3T3 mouse embryonic fibroblast cell line.

  2. How does Cultrex® Basement Membrane Extract (BME) promote cell differentiation?

    • All epithelial and endothelial cells are in contact with a basement membrane matrix on at least one of their surfaces. By providing them with their natural matrix in vitro as a substrate for the cells that provides biological cues, the cells can assume a more physiological morphology (i.e. correct shape) and begin expression of cell-lineage specific proteins. Two-dimensional plastic surfaces, in combination with serum-containing media, cause cells to flatten, proliferate and de-differentiate.

  3. How should Cultrex Basement Membrane Extract (BME) be stored and handled?

  4. What is the Tube Formation Assay?

    • The Tube Formation Assay is based on the ability of endothelial cells to form three-dimensional capillary-like tubular structures when cultured on a hydrogel of reconstituted basement membrane, such as Cultrex Basement Membrane Extract (BME).

  5. What are the advantages of the Tube Formation Assay?

    • The Tube Formation Assay is the most widely used in vitro angiogenesis assay. The assay is rapid, inexpensive and quantifiable. It can be used to identify potentially angiogenic and anti-angiogenic factors, to determine endothelial cell phenotype, and to study pathways and mechanisms involved in angiogenesis. It can be performed in a high throughput mode to screen for a large number of compounds.

  6. What cell types can be used in the Tube Formation Assay?

    • The Tube Formation Assay is specific for endothelial cells, either primary cells or immortalized cell lines. Only endothelial cells form capillary-like structures with a lumen inside. Other endothelial cell types form other structures.

  7. What are the variables associated with the Tube Formation Assay?

    • The major variables associated with tube formation are composition of the Cultrex Basement Membrane Extract (BME) hydrogel, thickness of the hydrogel, cell density, composition of angiogenic factors in the assay medium, and assay period.

  8. Which Cultrex Basement Membrane Extract (BME) should I use for the Tube Formation Assay?

    • Cultrex Reduced Growth Factor BME (RGF BME) is generally used for testing compounds that promote angiogenesis because formation of capillary-like structures (tubes) is significantly less compared to non-growth factor reduced varieties of Cultrex BME. The Cultrex In Vitro Angiogeneis Assay (Tube Formation) includes a qualified production lot of Cultrex RGF BME that exhibits reduced background tube formation in the absence of angiogenic factors.

  9. How do I reduce spontaneous formation of tubular structures on Cultrex BME in the absence of angiogenic factors?

    • Primary endothelial cells, such as Human Umbilical Vein Endothelial Cells (HUVECs) form capillary-like structures in the absence of added angiogenic factors less often than immortalized endothelial cells. Generally, reducing the number of cells per cm2 plated onto Cultrex BME will result in less background or spontaneous tube formation. Titrate the number of cells and find optimal conditions for your specific cell line. When endothelial cells fully form capillary structures in response to angiogenic activators, but not in their absence, you may proceed.

  10. Does Cultrex BME, Catalog # 3533-005-02, affect fluorescence readings when Alamar Blue is used for final readout of assay? 

    • BME is known to have autofluorescence, but if appropriate controls are evaluated, background can be successfully subtracted. A BME only control well with no cells should be used to subtract the background fluorescence.

View all FAQs

Reviews for Cultrex Reduced Growth Factor Basement Membrane Extract, Type 2, Pathclear

Average Rating: 5 (Based on 5 Reviews)

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Cultrex Reduced Growth Factor Basement Membrane Extract, Type 2, Pathclear
By Anonymous on 06/18/2022

dissolve it on ice, and don't put it outside for too not time

Cultrex Reduced Growth Factor Basement Membrane Extract, Type 2, Pathclear 3533-010-02
Cultrex Reduced Growth Factor Basement Membrane Extract, Type 2, Pathclear
By Anonymous on 02/21/2022

Aortic sprouting assay: mouse aortic ring cultured in a 48-well plate and imaged in an inverted microscope after 5 days.

Results are very consistent with this BME (used one layer under and another on top of the aortic ring).

Cultrex Reduced Growth Factor Basement Membrane Extract, Type 2, Pathclear 3533-010-02
Cultrex Reduced Growth Factor Basement Membrane Extract, Type 2, Pathclear
By Anonymous on 04/13/2021
Cultrex Reduced Growth Factor Basement Membrane Extract, Type 2, Pathclear
By Anonymous on 12/09/2020

We are using the BME at a final dilution of 1/30 for the maintenance of iPSC and differentiation into endoderm as well as mesoderm lineages. Attachment, growth, and differentiation of our iPSC is consistent, and we observed only minor variations between different lots.

Cultrex PathClear Reduced Growth Factor BME (2 x 5 mL)
By wenyi wu on 06/01/2018
Application: Cell migration/motility
Reason for Rating: it can form a very nice tube in our control group within 6-8 hour.
Cell migration/motility Cultrex PathClear Reduced Growth Factor BME (2 x 5 mL) 3533-010-02